Transcript: Human NM_014683.4

Homo sapiens unc-51 like autophagy activating kinase 2 (ULK2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
ULK2 (9706)
Length:
9149
CDS:
520..3630

Additional Resources:

NCBI RefSeq record:
NM_014683.4
NBCI Gene record:
ULK2 (9706)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147288 AGGCCCATGACGAGTAACCA pXPR_003 AGG 1867 60% 19 0.8611 ULK2 ULK2 77262
2 BRDN0001145207 TGGTCTGACGAGATGTTGTG pXPR_003 TGG 1074 35% 13 0.0798 ULK2 ULK2 77260
3 BRDN0001145025 TACCTTGCAAATAATCTGCG pXPR_003 AGG 281 9% 5 0.0713 ULK2 ULK2 77261
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014683.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196752 GATTGGGAGGTAGCTATTAAA pLKO.1 616 CDS 100% 15.000 21.000 N ULK2 n/a
2 TRCN0000342359 GATTGGGAGGTAGCTATTAAA pLKO_005 616 CDS 100% 15.000 21.000 N ULK2 n/a
3 TRCN0000000892 GCAGACCGAAGATATTGTTTA pLKO.1 3468 CDS 100% 13.200 18.480 N ULK2 n/a
4 TRCN0000352676 GCAGACCGAAGATATTGTTTA pLKO_005 3468 CDS 100% 13.200 18.480 N ULK2 n/a
5 TRCN0000000890 CTCATCTATAATTGTGCTGTA pLKO.1 3409 CDS 100% 4.050 5.670 N ULK2 n/a
6 TRCN0000196753 GCAGATTATTTGCAAGCGAAA pLKO.1 799 CDS 100% 4.050 5.265 N ULK2 n/a
7 TRCN0000195134 CCAATAGTCCTCAAGACTTAA pLKO.1 1166 CDS 100% 13.200 10.560 N ULK2 n/a
8 TRCN0000195035 CCTGTGACCTTAGGTTTATTA pLKO.1 4500 3UTR 100% 15.000 10.500 N ULK2 n/a
9 TRCN0000196773 GCAGAGAAACTCATCTATAAT pLKO.1 3400 CDS 100% 15.000 10.500 N ULK2 n/a
10 TRCN0000342360 GCAGAGAAACTCATCTATAAT pLKO_005 3400 CDS 100% 15.000 10.500 N ULK2 n/a
11 TRCN0000196407 GAGTTCTGACTGGTTCTTTAA pLKO.1 2271 CDS 100% 13.200 9.240 N ULK2 n/a
12 TRCN0000000891 GTCAGTGGTATTCGCATCAAA pLKO.1 964 CDS 100% 5.625 3.938 N ULK2 n/a
13 TRCN0000000889 GCAGTTTGATATTTGGAGTAA pLKO.1 4055 3UTR 100% 4.950 3.465 N ULK2 n/a
14 TRCN0000000893 TCCCAGAGAAACATCACCTTA pLKO.1 1227 CDS 100% 4.950 3.465 N ULK2 n/a
15 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 7237 3UTR 100% 5.625 2.813 Y KLHL30 n/a
16 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 7237 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014683.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14944 pDONR223 52.6% 99.3% 73.9% None (many diffs) n/a
2 ccsbBroad304_14944 pLX_304 0% 99.3% 73.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471790 GGAAGCATCCTTCCGAGCCTACCA pLX_317 13.5% 99.3% 73.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488021 CAAGCCTTGCCCACATCTTCCCGA pLX_317 13.4% 83% 21.2% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489251 TACACTTGTACATAAGTTAGCTTC pLX_317 13.3% 83% 21.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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