Transcript: Mouse NM_013881.4

Mus musculus unc-51 like kinase 2 (Ulk2), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ulk2 (29869)
Length:
5813
CDS:
517..3630

Additional Resources:

NCBI RefSeq record:
NM_013881.4
NBCI Gene record:
Ulk2 (29869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147288 AGGCCCATGACGAGTAACCA pXPR_003 AGG 1867 60% 19 0.8531 Ulk2 ULK2 77262
2 BRDN0001145207 TGGTCTGACGAGATGTTGTG pXPR_003 TGG 1074 34% 13 -0.1839 Ulk2 ULK2 77260
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013881.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000222262 CCCGAGGTTATTATGTCTCAA pLKO.1 1063 CDS 100% 4.950 6.930 N Ulk2 n/a
2 TRCN0000278670 CCCGAGGTTATTATGTCTCAA pLKO_005 1063 CDS 100% 4.950 6.930 N Ulk2 n/a
3 TRCN0000222261 CGCCATCTGAACATGATGTTA pLKO.1 3001 CDS 100% 5.625 4.500 N Ulk2 n/a
4 TRCN0000278669 CGCCATCTGAACATGATGTTA pLKO_005 3001 CDS 100% 5.625 4.500 N Ulk2 n/a
5 TRCN0000196773 GCAGAGAAACTCATCTATAAT pLKO.1 3400 CDS 100% 15.000 10.500 N ULK2 n/a
6 TRCN0000342360 GCAGAGAAACTCATCTATAAT pLKO_005 3400 CDS 100% 15.000 10.500 N ULK2 n/a
7 TRCN0000222265 CCAAAGACTCTGCGAGTAATA pLKO.1 1520 CDS 100% 13.200 9.240 N Ulk2 n/a
8 TRCN0000278672 CCAAAGACTCTGCGAGTAATA pLKO_005 1520 CDS 100% 13.200 9.240 N Ulk2 n/a
9 TRCN0000222263 CGCATAGAACAGAATCTTATA pLKO.1 1750 CDS 100% 13.200 9.240 N Ulk2 n/a
10 TRCN0000278671 CGCATAGAACAGAATCTTATA pLKO_005 1750 CDS 100% 13.200 9.240 N Ulk2 n/a
11 TRCN0000222264 GCAGACTGAAGACATCGTTTA pLKO.1 3468 CDS 100% 10.800 7.560 N Ulk2 n/a
12 TRCN0000297509 GCAGACTGAAGACATCGTTTA pLKO_005 3468 CDS 100% 10.800 7.560 N Ulk2 n/a
13 TRCN0000000893 TCCCAGAGAAACATCACCTTA pLKO.1 1224 CDS 100% 4.950 3.465 N ULK2 n/a
14 TRCN0000000890 CTCATCTATAATTGTGCTGTA pLKO.1 3409 CDS 100% 4.050 2.835 N ULK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013881.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14944 pDONR223 52.6% 89.9% 68.7% None (many diffs) n/a
2 ccsbBroad304_14944 pLX_304 0% 89.9% 68.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471790 GGAAGCATCCTTCCGAGCCTACCA pLX_317 13.5% 89.9% 68.7% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488021 CAAGCCTTGCCCACATCTTCCCGA pLX_317 13.4% 74.9% 20.9% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489251 TACACTTGTACATAAGTTAGCTTC pLX_317 13.3% 74.9% 20.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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