Construct: ORF ccsbBroad304_14954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018814.1_s304c1, BRDN0000459298
- Derived from:
- ccsbBroadEn_14954
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SGK2 (10110)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14954
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_001199264.1 | 99.5% | 35.2% | (many diffs) |
| 2 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_170693.3 | 99.5% | 35.2% | (many diffs) |
| 3 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_016276.3 | 85.6% | 30.3% | (many diffs) |
| 4 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_013731.3 | 88.6% | 33.3% | (many diffs) |
| 5 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_006499658.3 | 88.6% | 33.3% | (many diffs) |
| 6 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_001291152.1 | 88.4% | 33% | (many diffs) |
| 7 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_001291154.1 | 81.9% | 37.1% | (many diffs) |
| 8 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_011239580.2 | 81.9% | 37.1% | (many diffs) |
| 9 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_006499660.3 | 71.5% | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 549
- ORF length:
- 480
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactctagc ccagctggga ccccaagtcc acagccctcc agggccaatg 121 ggaacatcaa cctggggcct tcagccaacc caaatgccca gcccacggac ttcgacttcc 181 tcaaagtcat cggcaaaggg aactacggga aggtcctact ggccaagcgc aagtctgatg 241 gggcgttcta tgcagtgaag gtactacaga aaaagtccat cttaaagaag aaagagcaga 301 gccacatcat ggcagagcgc agtgtgcttc tgaagaacgt gcggcacccc ttcctcgtgg 361 gcctgcgcta ctccttccag acacctgaga agctctactt cgtgctcgac tatgtcaacg 421 gnggaggagc tcttcttcca cctgcagcgg gagcgccggt tcctggagcc ccgggcccag 481 gttctacgct gctgaggtgg ccagcgccat tggctacctg cactccctca acatcattta 541 cagggatctg aaaccagaga acattctctt ggactgccag ggacacgtgg tgctgacgga 601 ttttggcctc tgcaaggaag gtgtagagcc tgaagacacc acatccacat tctgtggtac 661 ccctgagtac ttggcacctg aagtgcttcg gaaagagcct tatgatcgag cagtggactg 721 gtggtgcttg ggggcagtcc tctacgagat gctccatggg cctgccgccc ttctacagcc 781 aagatgtatc ccagatgtat gagaacattc tgcaccagcc gctacagatc cccggaaggc 841 cggacagtgg ccgcctgtga cctcctgcaa agccttctcc acaaggacca gaggcagcgg 901 ctgggctcca aagcagactt tcttgagatt aagaaccatg tattcttcag ccccataaac 961 tgggatgacc tgtaccacaa gaggctaact ccacccttca acccaaatgt gacaggacct 1021 gctgacttga agcattttga cccagagttc acccaggaag ctgtgtccaa gtccattggc 1081 tgtacccctg acactgtggc cagcagctct ggggcctcaa gtgcattcct gggattttct 1141 tatgcgccag aggatgatga catcttggat tgcttgccaa ctttcttgta caaagtggtt 1201 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct 1261 aaccggtggc gcgttaagtc gacaatcaac ctctggatta