Construct: ORF ccsbBroad304_14981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000837.1_s304c1, BRDN0000399139
- Derived from:
- ccsbBroadEn_14981
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT2 (10965)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14981
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_006821.5 | 99.5% | 98.5% | (many diffs) |
| 2 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | NM_001037161.2 | 86% | 85.5% | (many diffs) |
| 3 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NR_046028.1 | 62.4% | (many diffs) | |
| 4 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364178.1 | 61.6% | 58.1% | (many diffs) |
| 5 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021590.1 | 59.5% | 56.3% | (many diffs) |
| 6 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364177.1 | 58% | 55.6% | (many diffs) |
| 7 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021589.1 | 57.5% | 55.6% | (many diffs) |
| 8 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021591.1 | 53.1% | 52.5% | (many diffs) |
| 9 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021592.1 | 53.1% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1515
- ORF length:
- 1449
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc taacaagctt ctttctcccc acccccattc agttgttctc aggtctgaat 121 tcaaaatggc ctcatctcct gctgtccttc gagcgtcccg gctgtaccaa tggagcctga 181 agagttcggc gcangtcctg gggtctccac agctgaggca ggttggtcag atcattaggg 241 ttcctgctcg gatggcggcg acgctgatcc tggagcctgc gggccgctgc tgctgggacg 301 aaccggtgcg aatcgccgtg cgcggcctag ccccggagca gccggtcacg ctgcgcgcgt 361 ccctgcgcga cgagaagggc gcgcttttcc aggcccacgc gcgctaccgc gccgacactc 421 ttggcgagct ggacctggag cgcgcgcccg cgctgggcgg cagcttcgcg gggcttgagc 481 ccatggggct gctctgggcc ttggagcccg agaaaccttt ggtgcggctg gtgaagcgcg 541 acgtgcgaac gcccttggcc gtggagctgg aggtgctgga tggccacgac cccgaccccg 601 ggcggctgct gtgccagacg cggcacgagc gctacttcct cccgcccggg gtgcggcgcg 661 agccggtgcg cgtgggccgg gtgcgaggca cgctcttcct gccgccagaa cctgggccct 721 ttcctgggat tgtggacatg ttcggaactg gaggtggcct gctggagttc gggnctagtc 781 tgctggctgg gaagggtttt gctgtgatgg ctctggctta ttataactat gaagacctcc 841 ccaagaccat ggagacgctc catctggagt actttgaaga agccatgaac tacttgctca 901 gtcatcccga ggtaaaaggt ccaggagttg ggctgcttgg aatttccaaa gggggtgagc 961 tctgcctttc catggcctct ttcctgaagg gcatcacggc tgctgtcgtc atcaacggct 1021 ctgtggccaa tgttggggga accttacgct acaagggcga gaccctgccc cctgtgggcg 1081 tcaacagaaa tcgcatcaag gtgaccaaag atggctatgc agacattgtg gatgtcctga 1141 acagcccttt ggaaggacct gaccagaaga gcttcattcc tgtggaaagg gcagagagca 1201 ccttcctgtt cctggtaggt caggatgacc acaactggaa gagtgagttc tatgctaatg 1261 aggcctgtaa acgcttgcag gcccatggga ggagaaagcc ccagatcatc tgttacccag 1321 agacagggca ctatattgag cctccttact tccccctgtg tcgggcttcc ctgcatgcct 1381 tggtgggcag tcctattatc tggggagggg agcccagggc tcatgtcatg gctcaggtgg 1441 acgcttggaa acaactccag actttcttcc acaaacactt gggtggccac gaggggacaa 1501 tcccatcaaa agtgtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1561 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1621 cgacaatcaa cctctggatt a