Transcript: Human NR_046028.1

Homo sapiens acyl-CoA thioesterase 2 (ACOT2), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2018-06-09
Taxon:
Homo sapiens (human)
Gene:
ACOT2 (10965)
Length:
1598
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_046028.1
NBCI Gene record:
ACOT2 (10965)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_046028.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426273 AGCCATGAACTACTTGCTCAG pLKO_005 802 3UTR 100% 2.250 1.575 N ACOT2 n/a
2 TRCN0000048892 GCTGTACCAATGGAGCCTGAA pLKO.1 569 3UTR 100% 4.050 2.430 N ACOT2 n/a
3 TRCN0000438195 TGGGATTGTGGACATGTTCGG pLKO_005 646 3UTR 100% 2.160 1.296 N ACOT2 n/a
4 TRCN0000256060 CCAGAGACAGGGCACTATATT pLKO_005 1238 3UTR 100% 15.000 7.500 Y ACOT1 n/a
5 TRCN0000256062 CTTGGTGGGCAGTCCTATTAT pLKO_005 1300 3UTR 100% 15.000 7.500 Y ACOT1 n/a
6 TRCN0000256061 TATGCTAATGAGGCCTGTAAA pLKO_005 1172 3UTR 100% 13.200 6.600 Y ACOT1 n/a
7 TRCN0000256063 CGCTCCATCTGGAGTACTTTG pLKO_005 777 3UTR 100% 10.800 5.400 Y ACOT1 n/a
8 TRCN0000048888 CCTGACCAGAAGAGCTTCATT pLKO.1 1079 3UTR 100% 5.625 2.813 Y ACOT2 n/a
9 TRCN0000048891 CGTCAACAGAAATCGCATCAA pLKO.1 1000 3UTR 100% 4.950 2.475 Y ACOT2 n/a
10 TRCN0000174083 CGTCAACAGAAATCGCATCAA pLKO.1 1000 3UTR 100% 4.950 2.475 Y ACOT2 n/a
11 TRCN0000179740 CGTCAACAGAAATCGCATCAA pLKO.1 1000 3UTR 100% 4.950 2.475 Y ACOT1 n/a
12 TRCN0000048889 GCACTATATTGAGCCTCCTTA pLKO.1 1249 3UTR 100% 4.950 2.475 Y ACOT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_046028.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14981 pDONR223 61.1% 62.4% None (many diffs) n/a
2 ccsbBroad304_14981 pLX_304 0% 62.4% V5 (many diffs) n/a
3 ccsbBroadEn_11575 pDONR223 100% 60.4% None (many diffs) n/a
4 ccsbBroad304_11575 pLX_304 0% 60.4% V5 (many diffs) n/a
5 TRCN0000474153 TCGCATTATGGCTAGCACCCCGAG pLX_317 29.2% 60.4% V5 (many diffs) n/a
6 ccsbBroadEn_05709 pDONR223 100% 60% None (many diffs) n/a
7 ccsbBroad304_05709 pLX_304 0% 60% V5 (many diffs) n/a
8 TRCN0000471582 GGATCAACTACTTCAAAAATGGGA pLX_317 37% 60% V5 (many diffs) n/a
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