Construct: ORF ccsbBroad304_15016

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018826.1_s304c1, BRDN0000459310
Derived from:
ccsbBroadEn_15016
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DAPK2 (23604)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15016
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23604 DAPK2 death associated protein ki... NM_014326.5 99.4% 33.5% (many diffs)
2 human 23604 DAPK2 death associated protein ki... XM_017022044.1 93.3% 35.4% (many diffs)
3 human 23604 DAPK2 death associated protein ki... XM_011521414.1 90.8% 32.6% (many diffs)
4 human 23604 DAPK2 death associated protein ki... XM_011521413.2 90.7% 31.8% (many diffs)
5 human 23604 DAPK2 death associated protein ki... XM_011521417.1 84.9% 39.2% (many diffs)
6 human 23604 DAPK2 death associated protein ki... XM_017022045.1 84.9% 39.2% (many diffs)
7 human 23604 DAPK2 death associated protein ki... XM_017022046.1 84.9% 39.2% (many diffs)
8 human 23604 DAPK2 death associated protein ki... XM_011521415.3 80.3% 34.5% (many diffs)
9 human 23604 DAPK2 death associated protein ki... NM_001363730.2 70.9% 25.4% (many diffs)
10 human 23604 DAPK2 death associated protein ki... XM_017022043.1 55.5% 21.4% (many diffs)
11 human 23604 DAPK2 death associated protein ki... XM_011521421.3 51.8% 10.7% (many diffs)
12 human 23604 DAPK2 death associated protein ki... XM_017022047.1 39.6% 6.2% (many diffs)
13 human 23604 DAPK2 death associated protein ki... XM_017022048.2 39.6% 6.2% (many diffs)
14 human 23604 DAPK2 death associated protein ki... XM_017022049.1 39.6% 6.2% (many diffs)
15 human 23604 DAPK2 death associated protein ki... XM_017022050.1 39.6% 6.2% (many diffs)
16 human 23604 DAPK2 death associated protein ki... XM_017022051.1 39.6% 6.2% (many diffs)
17 mouse 13143 Dapk2 death-associated protein ki... NM_010019.3 91.3% 32.1% (many diffs)
18 mouse 13143 Dapk2 death-associated protein ki... XM_006510811.2 52.6% 9.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
459
ORF length:
390
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gttccaggcc tcaatgagga gtccaaacat ggagccattc aagcagcaga
121aggtggagga cttttatgac atcggagagg agctggggag tggccagttt gccatcgtga
181agaagtgccg ggagaagagc acggggcttg agtatgcagc caagttcatc aagaagcggc
241agagccgggc gagccggcgc ggtgtgagcc gggaggagat cgagcgggag gtgagcatcc
301tgcggcaggt gctgcaccac aatgtcatca cgctgcacga cgtctatgag aaccgcaccg
361acgtggtgct catccttgag ctagtgtctg gaggagagct cttcgatttc ctggcccaga
421aggagtcact gagtgangan gangccaccc agcttcatta agcagatcct ggatggggtg
481aactaccttc acacaaagaa aattgctcac tttgatctca agccagaaaa cattatgttg
541ttagacaaga atattcccat tccacacatc aagctgattg actttggtct ggctcacgaa
601atagaagatg gagttgaatt taagaatatt tttggggacg ccggaatttg ttgctccaga
661aattgtgaac tacgagcccc tggggtctgg aggctgacat gtggagcata ggcgtcatca
721cctacatcct cttaagtgga gcatcccctt tcctgggaga cacgaagcag gaaacactgg
781caaatatcac agcagtgagt tacgactttg atgaggaatt cttcagccag acgagcgagc
841tggccaagga ctttattcgg aagcttctgg ttaaagagac ccggaaacgg ctcacaatcc
901aagaggctct cagacacccc tggatcacgc cggtggacaa ccagcaagcc atggtgcgca
961gggagtctgt ggtcaatctg gagaacttca ggaagcagta tgtccgcagg cggtggaagc
1021tttccttcag catcgtgtcc ctgtgcaacc acctcacccg ctcgctgatg aagaaggtgc
1081acctgaggcc ggatgaggac ctgaggaact gtgagagtga cactgaggag gacatcgcca
1141ggaggaaagc cctccaccca cggaggagga gcagcacctc cttgccaact ttcttgtaca
1201aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1261ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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