Construct: ORF ccsbBroad304_15152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018928.1_s304c1, BRDN0000459412
- Derived from:
- ccsbBroadEn_15152
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PEAK1 (79834)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15152
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | NM_024776.4 | 90.7% | 6.2% | (many diffs) |
| 2 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_005254670.2 | 90.7% | 6.2% | (many diffs) |
| 3 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_005254671.2 | 90.7% | 6.2% | (many diffs) |
| 4 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_005254673.2 | 90.7% | 6.2% | (many diffs) |
| 5 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_005254674.2 | 90.7% | 6.2% | (many diffs) |
| 6 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_005254675.4 | 90.7% | 6.2% | (many diffs) |
| 7 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522033.2 | 90.7% | 6.2% | (many diffs) |
| 8 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522034.2 | 90.7% | 6.2% | (many diffs) |
| 9 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522035.2 | 90.7% | 6.2% | (many diffs) |
| 10 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522036.2 | 90.7% | 6.2% | (many diffs) |
| 11 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522037.2 | 90.7% | 6.2% | (many diffs) |
| 12 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522038.2 | 90.7% | 6.2% | (many diffs) |
| 13 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522039.2 | 90.7% | 6.2% | (many diffs) |
| 14 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450056.1 | 90.7% | 6.2% | (many diffs) |
| 15 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450057.1 | 90.7% | 6.2% | (many diffs) |
| 16 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450058.1 | 90.7% | 6.2% | (many diffs) |
| 17 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450059.1 | 90.7% | 6.2% | (many diffs) |
| 18 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450060.1 | 90.7% | 6.2% | (many diffs) |
| 19 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450061.1 | 90.7% | 6.2% | (many diffs) |
| 20 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450062.1 | 90.7% | 6.2% | (many diffs) |
| 21 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450063.1 | 90.7% | 6.2% | (many diffs) |
| 22 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_024450064.1 | 90.7% | 6.2% | (many diffs) |
| 23 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522040.1 | 69.3% | 7.9% | (many diffs) |
| 24 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522041.1 | 51.6% | 10.3% | (many diffs) |
| 25 | human | 79834 | PEAK1 | pseudopodium enriched atypi... | XM_011522042.2 | 41.5% | 3.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 480
- ORF length:
- 411
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaan 61 tnggcaccat gtctgcttgt aacaccttta ctgaacatgt ttggaaacct ggtgaatgca 121 agaattgctt taaacctaaa agtttgcacc agcttccccc agaccctgag aaggcaccca 181 tcacccatgg caatgtgaaa actaatgcca atcacagtaa caaccaccgc atcaggaaca 241 cgggcaattt ccggcctcct gtggctaaaa aacccactat agctgtgaag cccactatga 301 tagtggcaga tgggcaaagt atatgtggtg agcttagcat ccaagaacac tgtgagaaca 361 aacctgtcat catagggtgg aaccgaaaag cggatcctca tttcagacgc caaaggcatc 421 ctccagtgtc tgctctgggg cccccgcgaa gatctcttcc agactttcac cgcctgccct 481 agcctagtac agaggaacac cctgctccaa aactggctag acatcaagcg aacactgctc 541 atgatcaagt ttgctgagaa gtccctggac agggaaggtg gaatcagcct tgaggactgg 601 ctttgtgctc agtatttggc ttttgccact acagactccc tcagttgtat tgtgaaaatt 661 ctgcagcacc gtttgccaac tttcttgtac aaagttggca ttataagaaa gcnnnnnttn 721 ncnanntgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat tgccatcnng 781 nnngattcaa agttggcatt ataagaaagc anccccctgc ttatcaattt gttgcaacga 841 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccaggctgat atccccgtat 901 agtgagtcgt attacatgga tcatagctgt ttcctggcag ctctgggccc gtgtctcaat 961 ccacatcacc agattcttct ttaacagaag aatcacgttc tgagacagcc agtagtttat 1021 cccagaagat ttgtaatggg ggattatctc ctggtaaccc aggagattct aaggacatga 1081 aggaaattga gcccaattat gaaagtccct ctagtaataa tcaggataaa gattcatcac 1141 aggcttccaa aagctcaata aaagttccag agacccacaa agcagtcctt gctctccgat 1201 tagaagagaa agatggcaag attgctgtac aaactgagaa ggaagaaagt aaagcctcta 1261 cagatgttgc tgggcaagca gtaaccataa accttgtccc cacagaagag caagcaaaac 1321 cttaccgagt tgtgaacctg gaacagccat tgtgcaagcc atatactgtc gtggatgtgt 1381 cagcagccat ggccagtgag cacctcgagg gccctgttaa cagccccaag acaaaaagct 1441 catcctctac tccaaactct ccagttacat catcttcatt gacaccagga caaataagtg 1501 cccatttcca aaaatccagt gcaattcgat accaagaagt atggacttct agcaccagtc 1561 cacgacaaaa gatacctaaa gtagaactaa ttactagtgg aactggacca aatgttcctc 1621 caaggaaaaa ctgtcacaaa tcagcaccta catcacccac agctacaaac atttcctcca 1681 aaaccatccc tgttaagtca cctaatttgt ctgaaattaa atttaatagt tataacaatg 1741 ctggtatgcc accttttcca attatcattc atgacgagcc aacttatgct cggagttcca 1801 aaaatgctat caaagttccc attgttatca atccaaatgc atatgacaat ctagctatct 1861 acaaaagttt tctgggaaca agtggagaac tctcagtgaa ggaaaaaacc acaagtgtaa 1921 taagccatac ttatgaagaa atagaaacag aaagcaaagt gcctgataac accactagca 1981 aaaccactga ctgtcttcaa actaaagggt tttcaaacag cacagagcat aaaaggggct 2041 cagtggctca gaaggttcaa gagtttaaca actgtctcaa cagaggtcag tcttcaccac 2101 agagaagcta tagttccagc cacagctccc cagcaaagat ccagagagcc actcaagagc 2161 ctgtggccaa aatagaaggc actcaggagt ctcagatggt gggcagcagc agcaccagag 2221 agaaagcaag cacagtgctt tctcagattg tggcttcaat ccaaccccca cagtctcctc 2281 cagaaacacc tcaatctggc cctaaagctt gcagtgtgga agagctttat gccattcctc 2341 cagatgctga tgttgctaag agcacaccta agagtacgcc agtccggccc aaatctctct 2401 ttacatctca gcctagtggt gaggctgaag cacctcagac cacagacagt cctaccacca 2461 aagtacagaa agacccatcc ataaagccag tcaccccctc tccctccaaa ttagtgacta 2521 gcccccaaag tgagccacca gctccctttc ccccgccacg ctctacttct tctccttacc 2581 atgcaggtaa ccttttgcag aggcatttca ccaactggac caagccaacc agccctacca 2641 ggtcaacaga agctgaatca gttttgcact ctgaaggcag caggcgggca gctgatgcaa 2701 aacctaagcg ctggatatca tttaaaagct tcttccgccg tcggaaaaca gatgaggagg 2761 atgacaaaga gaaagagcga gagaaaggga aactggtggg cctggatggc acagtcattc 2821 acatgctgcc tcctcctcca gttcagcgcc atcactggtt cacagaggcg aaaggagagt 2881 ccagtgagaa accagccatt gtcttcatgt acaggtgcga ccctgctcaa ggccagctca 2941 gtgtggatca gagcaaggct aggacagacc aggcagcagt catggagaag ggtagagcag 3001 agaatgcatt actacaggac tcagagaaga agaggagtca ttcttctcca tcacagattc 3061 ctaaaaagat tctcagtcac atgacccatg aagtaacaga ggatttttct cctcgggatc 3121 caagaactgt tgttgggaag caagatggca ggggctgcac ttcagtcaca acagcattgt 3181 ccctacctga actggaaagg gaagatggaa aagaagacat ttcagatcct atggacccga 3241 acccttgtag tgcaacatac agcaacttag ggcaatctag agcagccatg atacctccca 3301 agcagccacg acagcccaag ggagctgtgg acgatgccat cgcctttgga gggaaaacag 3361 accaagaagc acccaatgct tcccaaccta caccaccccc actgccaaag aagatgatca 3421 taagagccaa tacagagcca atctccaagg acctccaaaa atccatggaa agtagtcttt 3481 gtgtcatggc taatcccacc tatgatatcg accccaactg ggatgccagc agtgctggtt 3541 cttccatcag ctatgaactc aaaggactgg acattgagtc ttatgactcc ttggaaaggc 3601 ctttgcgcaa ggagagacct gtcccctcag cagcaaacag catttccagc ttaaccactc 3661 tcagtattaa ggatagattt tccaacagca tggaatccct ctccagccgg cgtgggccct 3721 cttgcagaca gggccgaggc atccagaagc cgcagagaca agcactttat cgaggacttg 3781 agaatcggga ggaagtagtg ggtaaaatcc gaagccttca tacagatgcc ttgaagaaac 3841 tggctgttaa atgcgaagac cttttcatgg ctgggcagaa agaccagctc cgttttggag 3901 tggacagctg gtcagacttc aggctaacca gtgacaaacc atgttgtgag gcaggtgatg 3961 cggtttacta tactgcttca tatgcaaaag atccacttaa taactatgca gtcaagatct 4021 gtaagagcaa agctaaagaa tctcagcagt attatcacag cttggctgtc cggcagagtc 4081 tggctgtcca ttttaacatt cagcaggact gtggtcattt ccttgctgaa gtccctaacc 4141 gtctgcttcc ctgggaggat ccagatgacc ctgaaaagga tgaggatgac atggaagaga 4201 ctgaagaaga cgccaaagga gaaacggatg ggaaaaaccc aaagccctgt tctgaagcag 4261 catcatccca gaaagagaat cagggagtca tgagcaagaa gcagaggagc cacgttgtgg 4321 tcatcaccag ggaggttcca tgtcttactg tggctgattt tgtgcgagac tctctggccc 4381 agcatgggaa aagccctgat ttgtatgaga ggcaggtgtg tctgctgctc ttacagctat 4441 gctctggtct tgagcacctc aaaccctacc atgtcactca ctgcgatcta cgcctagaga 4501 acctgctact tgtccactac cagcctgggg ggactgccca aggctttggg cctgcagagc 4561 ccagccccac ctcatcttat cccactaggc ttatagtgag caacttctct caggccaagc 4621 agaagagcca tctggtggac cccgagatcc tccgggacca gtctcgcctt gccccagaga 4681 tcataacagc tacccagtat aaaaagtgtg atgagttcca gacaggcatc ctcatctatg 4741 agatgctgca cctacccaac ccctttgatg agaacccaga gctgaaggag agggaataca 4801 cacgagcaga cctgcctcgc atcccattcc gctcccccta ctcccggggt ctgcagcagc 4861 tggccagctg cctcctgaat cccaaccctt ctgagcggat cctcatttca gacgccaaag 4921 gcatcctcca gtgtctgctc tggggccccc gcgaagatct cttccagact ttcaccgcct 4981 gccctagcct agtacagagg aacaccctgc tccaaaactg gctagacatc aagcgaacac 5041 tgctcatgat caagtttgct gagaagtccc tggacaggga aggtggaatc agccttgagg 5101 actggctttg tgctcagtat ttggcttttg ccactacaga ctccctcagt tgtattgtga 5161 aaattctgca gcaccgtttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 5221 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 5281 agtcgacaat caacctctgg atta