Construct: ORF ccsbBroad304_15254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018831.1_s304c1, BRDN0000459315
- Derived from:
- ccsbBroadEn_15254
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NEK7 (140609)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15254
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140609 | NEK7 | NIMA related kinase 7 | NM_133494.3 | 99% | 15.2% | (many diffs) |
| 2 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000344.1 | 96.4% | 14.8% | (many diffs) |
| 3 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000345.1 | 96.4% | 14.8% | (many diffs) |
| 4 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000346.1 | 84.6% | 16.9% | (many diffs) |
| 5 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000347.1 | 67.3% | 20.3% | (many diffs) |
| 6 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_017000348.1 | 67.3% | 20.3% | (many diffs) |
| 7 | human | 140609 | NEK7 | NIMA related kinase 7 | XM_011509209.1 | 52.5% | 28.7% | (many diffs) |
| 8 | mouse | 59125 | Nek7 | NIMA (never in mitosis gene... | NM_021605.4 | 90.4% | 14.9% | (many diffs) |
| 9 | mouse | 59125 | Nek7 | NIMA (never in mitosis gene... | NM_001311148.1 | 60.2% | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 207
- ORF length:
- 138
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgagcaa tcacaaggaa tgcaagggcc acctgttcct cagttccaac 121 cacagaaggc cttacgaccg gatatgggct ataatacatt agccaacttt cgaatagaaa 181 agaaaattgg tcgcggacaa tttagttgaa gtttatagag cagcctgtct ctnggatgga 241 gtaccagtag ctttaaaaaa agtgcagata tttgnnttaa tggatgccaa agcacgtgct 301 gattgcatca aagaaataga tcttcttaag caactcaacc atccaaatgt aataaaatat 361 tatgcatcat tcattgaaga taatgaacta aacatagttt tggaactagc agatgctggc 421 gacctatcca gaatgatcaa gcattttaag aagcaaaaga ggctaattcc tgaaagaact 481 gtttggaagt attttgttca gctttgcagt gcattggaac acatgcattc tcgaagagtc 541 atgcatagag atataaaacc agctaatgtg ttcattacag ccactggggt ggtaaaactt 601 ggagatcttg ggcttggccg gtttttcagc tcaaaaacca cagctgcaca ttctttagtt 661 ggtacgcctt attacatgtc tccagagaga atacatgaaa atgggataca acttcaaatc 721 tgacatctgg tctcttggct gtctactata tgagatggct gcattacaaa gtcctttcta 781 tggtgacaaa atgaatttat actcactgtg taagnagata gaacagtgtg actacccacc 841 tctnncttca gatcactatt cagaagnact ccgacagtta gttaatatgt gcatcaaccc 901 agatccagag aagcgaccag acgtcaccta tgtttatgac gtagcaaaga ggatgcatgc 961 atgcactgca agcagcttgc caactttctt gtacaaagtg gttggtaagc ctatccctaa 1021 ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa 1081 gtcgacaatc aacctctgga tta