Construct: ORF ccsbBroad304_15268

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018872.1_s304c1, BRDN0000459356
Derived from:
ccsbBroadEn_15268
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
NEK10 (152110)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15268
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 152110 NEK10 NIMA related kinase 10 NM_001031741.3 89.5% 36.4% (many diffs)
2 human 152110 NEK10 NIMA related kinase 10 NM_001304384.1 87.6% 35.7% (many diffs)
3 human 152110 NEK10 NIMA related kinase 10 XM_024453374.1 58.2% 23.1% (many diffs)
4 human 152110 NEK10 NIMA related kinase 10 XM_017005772.2 38.2% 15% (many diffs)
5 human 152110 NEK10 NIMA related kinase 10 XM_006713001.3 37.2% 14.7% (many diffs)
6 human 152110 NEK10 NIMA related kinase 10 XM_017005771.2 37.2% 14.7% (many diffs)
7 human 152110 NEK10 NIMA related kinase 10 XM_017005769.1 37.2% 14.6% (many diffs)
8 human 152110 NEK10 NIMA related kinase 10 XM_017005770.2 37.2% 14.6% (many diffs)
9 human 152110 NEK10 NIMA related kinase 10 XM_017005768.1 37.2% 14.6% (many diffs)
10 human 152110 NEK10 NIMA related kinase 10 XM_017005767.2 36.6% 14.4% (many diffs)
11 human 152110 NEK10 NIMA related kinase 10 XM_024453373.1 36.6% 14.4% (many diffs)
12 human 152110 NEK10 NIMA related kinase 10 XM_006712997.3 36.1% 14.1% (many diffs)
13 human 152110 NEK10 NIMA related kinase 10 XM_017005766.2 36.1% 14.1% (many diffs)
14 human 152110 NEK10 NIMA related kinase 10 NM_152534.4 35.7% 14% (many diffs)
15 human 152110 NEK10 NIMA related kinase 10 XM_017005761.2 35.7% 14% (many diffs)
16 human 152110 NEK10 NIMA related kinase 10 XM_017005762.2 35.7% 14% (many diffs)
17 human 152110 NEK10 NIMA related kinase 10 XM_017005763.1 35.7% 14% (many diffs)
18 human 152110 NEK10 NIMA related kinase 10 XM_017005764.1 35.7% 14% (many diffs)
19 human 152110 NEK10 NIMA related kinase 10 XM_017005765.1 35.7% 14% (many diffs)
20 human 152110 NEK10 NIMA related kinase 10 XM_006712998.2 34.7% 14.1% (many diffs)
21 human 152110 NEK10 NIMA related kinase 10 XM_006712999.3 34.5% 14.2% (many diffs)
22 human 152110 NEK10 NIMA related kinase 10 XM_017005773.1 22% 17.4% (many diffs)
23 human 152110 NEK10 NIMA related kinase 10 XR_001740034.1 19.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
624
ORF length:
555
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggttcctgta cagcctgcag aaccccccga ggtactgaag agtgagccgt
121atggggagaa ggctgatgtc tgggcagtag gctgcatcct ttatcagatg gcgactttga
181gtcccccctt ctacagcact aacatgctgt ccttggctac aaaaatagtg gaggcggtat
241atgaaccagt gccagaaggt atctactctg aaaaagtaac agacaccatc agcaggtgcc
301tcactcctga tgcggaagct cgtccagata ttgtagaagt cagttcgatg atatcagatg
361tcatgatgaa atatttagac aacttatcta catcccagtt gtccttggaa aagaagctag
421aacgggaacg aagacgcaca caaaggtatt ttatggaagc caaccggaac accgtcacat
481gtcaccatga gctggctgtt ctatctcacg agacctttga gaaggcaagt ttgagtagca
541ncagcnnngg ancagccanc ctgaaaantg aactttcana aagcgcagac ctgccccctg
601naggctttcc aggcctccta tggtaaagac gaagacaggg cctgtgacga aatcctgtca
661gatgataact tcaacctgga aaatgctgag aaagatacat attcagaggt agatgatgaa
721ttggacattt cggataactc cagcagctcc agttcaagcc ctctgaaagn atctacattc
781aacattttaa agagaagttt tagtgcttca ggaggagaaa gacannccca aannagggac
841ttcactggag gaacaggatc aagaccaaga ccagacagac agcatcagca ggaattgctg
901tgtcccagag gaaagtgcgt cagatcagtg atcctattca gcagatatta attcagctgc
961acaaaataat ctatatcaca cagcttcctc cagctttgca ccacaatttg aaaagaaggg
1021ttatagagag attcaagaaa tccctcttca gccagcagag taacccttgt aatttgaaat
1081ctgaaattaa aaagttatct cagggatctc cagaaccgat tgagcccaac tttttcacag
1141cagattacca tttattacat cgttcatccg gtggaaacag cctgtcccca aatgacccta
1201caggtttacc aaccagcatt gaattggagg aaggaataac atatgaacag atgcagactg
1261tgattgaaga agtccttgag gaaagtggct attacaattt tacatctaac aggtatcatt
1321cctatccatg ggggaccaag aatcacccaa ccaaaagatt gccaactttc ttgtacaaag
1381tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1441gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

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