Construct: ORF ccsbBroad304_15398

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004233.1_s304c1, BRDN0000409521
Derived from:
ccsbBroadEn_15398
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DKC1 (1736)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15398
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1736 DKC1 dyskerin pseudouridine synt... NM_001363.5 99.8% 99.8% 369G>T;853G>T
2 human 1736 DKC1 dyskerin pseudouridine synt... NM_001142463.3 98.8% 98.6% 369G>T;853G>T;1244_1245insGTATGTTGACTACAG
3 human 1736 DKC1 dyskerin pseudouridine synt... NM_001288747.1 81.5% 81.3% 853G>T;1260_1260delGins283
4 human 1736 DKC1 dyskerin pseudouridine synt... NR_110023.2 54.2% (many diffs)
5 human 1736 DKC1 dyskerin pseudouridine synt... NR_110021.2 52.2% (many diffs)
6 human 1736 DKC1 dyskerin pseudouridine synt... NR_110022.2 50.2% (many diffs)
7 mouse 245474 Dkc1 dyskeratosis congenita 1, d... NM_001030307.2 87.9% 90.8% (many diffs)
8 mouse 245474 Dkc1 dyskeratosis congenita 1, d... XM_006528023.3 83.4% 85.5% (many diffs)
9 mouse 245474 Dkc1 dyskeratosis congenita 1, d... XM_017318488.1 51.9% 53.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1608
ORF length:
1542
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggatgcggaa gtaattattt tgccaaagaa acataagaag aaaaaggagc
121ggaagtcatt gccagaagaa gatgtagccg aaatacaaca cgctgaagaa tttcttatca
181aacctgaatc caaagttgct aagttggaca cgtctcagtg gccccttttg ctaaagaatt
241ttgataagct gaatgtaagg acaacacact atacacctct tgcatgtggt tcaaatcctc
301tgaagagaga gattggggac tatatcagga caggtttcat taatcttgac aagccctcta
361acccctcttc ccatgaggtg gtagcctgga ttcgacggat acttcgggtg gagaagacag
421ggcacagtgg tactctggat cccaaggtga ctggttgttt aatcgtgtgc atagaacgag
481ccactcgctt ggtgaagtca caacagagtg caggcaaaga gtatgtgggg attgtccggc
541tgcacaatgc tattgaaggg gggacccagc tttctagggc cctagaaact ctgacaggtg
601ccttattcca gcgaccccca cttattgctg cagtaaagag gcagctccga gtgaggacca
661tctacgagag caaaatgatt gaatacgatc ctgaaagaag attaggaatc ttttgggtga
721gttgtgaggc tggcacctac attcggacat tatgtgtgca ccttggtttg ttattgggag
781ttggtggtca gatgcaggag cttcggaggg ttcgttctgg agtcatgagt gaaaaggacc
841acatggtgac aatgcatgat gtgcttgatg ctcagtggct gtatgataac cacaaggatg
901agagttacct gcggcgattt gtttaccctt tggaaaagct gttgacatct cataaacggc
961tggttatgaa agacagtgca gtaaatgcca tctgctatgg ggccaagatt atgcttccag
1021gtgttcttcg atatgaggac ggcattgagg tcaatcagga gattgtggtt atcaccacca
1081aaggagaagc aatctgcatg gctattgcat taatgaccac agcggtcatc tctacctgcg
1141accatggtat agtagccaag atcaagagag tgatcatgga gagagacact taccctcgga
1201agtggggttt aggtccaaag gcaagtcaga agaagctgat gatcaagcag ggccttctgg
1261acaagcatgg gaagcccaca gacagcacac ctgccacctg gaagcaggag tatgttgact
1321acagtgagtc tgccaaaaaa gaggtggttg ctgaagtggt aaaagccccg caggtagttg
1381ccgaagcagc aaaaactgcg aagcggaagc gagagagtga gagtgaaagt gacgagactc
1441ctccagcagc tcctcagttg atcaagaagg aaaagaagaa gagtaagaag gacaagaagg
1501ccaaagctgg tctggagagc ggggccgagc ctggagatgg ggacagtgat accaccaaga
1561agaagaagaa gaagaagaaa gcaaaagagg tagaattggt ttctgagtnn ccaactttct
1621tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1681aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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