Construct: ORF ccsbBroad304_15514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000238.1_s304c1, BRDN0000400037
- Derived from:
- ccsbBroadEn_15514
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OAT (4942)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15514
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4942 | OAT | ornithine aminotransferase | NM_000274.4 | 99.9% | 100% | 1134C>T |
| 2 | human | 4942 | OAT | ornithine aminotransferase | NM_001322965.2 | 99.9% | 100% | 1134C>T |
| 3 | human | 4942 | OAT | ornithine aminotransferase | NM_001322966.2 | 99.9% | 100% | 1134C>T |
| 4 | human | 4942 | OAT | ornithine aminotransferase | NM_001322967.2 | 99.9% | 100% | 1134C>T |
| 5 | human | 4942 | OAT | ornithine aminotransferase | NM_001322968.2 | 99.9% | 100% | 1134C>T |
| 6 | human | 4942 | OAT | ornithine aminotransferase | NM_001322969.2 | 99.9% | 100% | 1134C>T |
| 7 | human | 4942 | OAT | ornithine aminotransferase | NM_001322970.2 | 99.9% | 100% | 1134C>T |
| 8 | human | 4942 | OAT | ornithine aminotransferase | NM_001322971.2 | 75.5% | 75.6% | 197_198ins321;813C>T |
| 9 | human | 4942 | OAT | ornithine aminotransferase | NM_001171814.1 | 68.4% | 68.5% | 0_1ins414;720C>T |
| 10 | human | 4942 | OAT | ornithine aminotransferase | NM_001322974.2 | 54.3% | 54.4% | 0_1ins600;534C>T |
| 11 | human | 4942 | OAT | ornithine aminotransferase | XM_017016279.1 | 54.3% | 54.4% | 0_1ins600;534C>T |
| 12 | mouse | 18242 | Oat | ornithine aminotransferase | NM_016978.2 | 84.5% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttccaaacta gcacatttgc agaggtttgc tgtacttagt cgcggagttc 121 attcttcagt ggcttctgct acatctgttg caactaaaaa aacagtccaa ggccctccaa 181 cctctgatga catttttgaa agggaatata agtatggtgc acacaactac catcctttac 241 ctgtagccct ggagagagga aaaggtattt acttatggga tgtagaaggc agaaaatatt 301 ttgacttcct gagttcttac agtgctgtca accaagggca ttgtcacccc aagattgtga 361 atgctctgaa gagtcaagtg gacaaattga ccttaacatc tagagctttc tataataacg 421 tacttggtga atatgaggag tatattacta aacttttcaa ctaccacaaa gttcttccta 481 tgaatacagg agtggaggct ggagagactg cctgtaaact agctcgtaag tggggctata 541 ccgtgaaggg cattcagaaa tacaaagcaa agattgtttt tgcagctggg aacttctggg 601 gtaggacgtt gtctgctatc tccagttcca cagacccaac cagttacgat ggttttggac 661 catttatgcc gggattcgac atcattccct ataatgatct gcccgcactg gagcgtgctc 721 ttcaggatcc aaatgtggct gcgttcatgg tagaaccaat tcagggtgaa gcaggcgttg 781 ttgttccgga tccaggttac ctaatgggag tgcgagagct ctgcaccagg caccaggttc 841 tctttattgc tgatgaaata cagacaggat tggccagaac tggtagatgg ctggctgttg 901 attatgaaaa tgtcagacct gatatagtcc tccttggaaa ggccctttct gggggcttat 961 accctgtgtc tgcagtgctg tgtgatgatg acatcatgct gaccattaag ccaggggagc 1021 atgggtccac atacggtggc aatccactag gctgccgagt ggccatcgca gcccttgagg 1081 ttttagaaga agaaaacctt gctgaaaatg cagacaaatt gggcattatc ttgagaaatg 1141 aactcatgaa gctaccttct gatgttgtaa ctgccgtaag aggaaaagga ttattaaatg 1201 ctattgtcat taaagaaacc aaagattggg atgcttggaa ggtgtgtcta cgacttcgag 1261 ataatggact tctggccaag ccaacccatg gcgacattat caggtttgcg cctccgctgg 1321 tgatcaagga ggatgagctt cgagagtcca ttgaaattat taacaagacc atcttgtctt 1381 tctnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1441 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1501 tctggatta