Construct: ORF ccsbBroad304_15716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013066.1_s304c1, BRDN0000407537
- Derived from:
- ccsbBroadEn_15716
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4B (10509)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15716
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324030.2 | 69.4% | 69.4% | (many diffs) |
| 2 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324033.2 | 69.4% | 69.4% | (many diffs) |
| 3 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324029.2 | 67.6% | 67.6% | (many diffs) |
| 4 | human | 10509 | SEMA4B | semaphorin 4B | NM_020210.4 | 56.5% | 56.5% | (many diffs) |
| 5 | human | 10509 | SEMA4B | semaphorin 4B | NM_198925.3 | 56.5% | 56.5% | (many diffs) |
| 6 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324034.2 | 55.8% | 55.8% | (many diffs) |
| 7 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324031.2 | 53.1% | 53.1% | (many diffs) |
| 8 | human | 10509 | SEMA4B | semaphorin 4B | NM_001324032.2 | 27.5% | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggatgtgcag agagtcttca gcggcctcta caaggaggtg aaccgtgaga 121 cacagcagtg gtacaccgtg acccacccgg tgcccacacc ccggcctgga gcgtgcatca 181 ccaacagtgc ccgggaaagg aagatcaact catccctgca gctcccagac cgcgtgctga 241 actttctcaa ggaccacttc ctgatggacg ggcaggtccg aagccgcatg ctgctgctgc 301 agccccaggc tcgctaccag cgcgtggctg tacaccgcgt ccctggcctg caccacacct 361 acgatgtcct cttcctcggc actggtgacg gccggctcca caaggcagtg agcgtgggcc 421 cccgggtgca catcattgag gagctgcaga tcttctcatc gggacagccc gtgcagaatc 481 tgctcctgga cacccacagg gggctgctgt atgcggcctc acactcgggc gtagtccagg 541 tgcccatggc caactgcagc ctgtaccgga gctgtgggga ctgcctcctc gcccgggacc 601 cctactgtgc ttggagcggc tccagctgca agcacgtcag cctctaccag cctcagctgg 661 ccaccaggcc gtggatccag gacatcaagg gagccagcgc caaggacctt tgcagcgcgt 721 cttcggttgt gtccccgtct tttgtaccaa caggggagaa gccatgtgag caagtccagt 781 tccagcccaa cacagtgaac actttggcct gcccgctcct ctccaacctg gcgacccgac 841 tctggctacg caacggggcc cccgtcaatg cctcggcctc ctgccacgtg ctacccactg 901 gggacctgct gctggtgggc acccaacagc tgggggagtt ccagtgctgg tcactagagg 961 agggcttcca gcagctggta gccagctact gcccagaggt ggtggaggac ggggtggcag 1021 accaaacaga tgagggtggc agtgtacccg tcattatcag cacatcgcgt gtgagtgcac 1081 cagctggtgg caaggccagc tggggtgcag acaggtccta ctggaaggag ttcctggtga 1141 tgtgcacgct ctttgtgctg gccgtgctgc tcccagtttt attcttgctc taccggcacc 1201 ggaacagcat gaaagtcttc ctgaagcagg gggaatgtgc cagcgtgcac cccaagacct 1261 gccctgtggt gctgccccct gagacccgcc cactcaacgg cctagggccc cctagcaccc 1321 cactcgatca ccgagggtac cagtccctgt cagacagccc cccgggggcc cgagtcttca 1381 ctgagtcaga gaagaggcca ctcagcatcc aagacagctt cgtggaggta tccccagtgt 1441 gcccccggcc ccgggtccgc cttggctcgg agatccgtga ctctgtggtg tnnccaactt 1501 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1561 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta