Construct: ORF ccsbBroad304_15730

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012916.1_s304c1, BRDN0000404837
Derived from:
ccsbBroadEn_15730
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PNPLA6 (10908)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15730
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10908 PNPLA6 patatin like phospholipase ... NM_001166113.1 99.9% 99.8% 1090G>C;1206G>A;2954A>G
2 human 10908 PNPLA6 patatin like phospholipase ... NM_006702.5 99.9% 99.8% 1090G>C;1206G>A;2954A>G
3 human 10908 PNPLA6 patatin like phospholipase ... NM_001166112.1 97.8% 97.8% (many diffs)
4 human 10908 PNPLA6 patatin like phospholipase ... NM_001166114.2 96.7% 96.2% (many diffs)
5 human 10908 PNPLA6 patatin like phospholipase ... NM_001166111.2 96.4% 96.3% (many diffs)
6 mouse 50767 Pnpla6 patatin-like phospholipase ... NM_015801.2 86.5% 96% (many diffs)
7 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508825.3 86.5% 96% (many diffs)
8 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508830.3 86.2% 95.6% (many diffs)
9 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_017312890.1 86.2% 95.6% (many diffs)
10 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_011242066.2 85.4% 91.3% (many diffs)
11 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_017312891.1 85.1% 91% (many diffs)
12 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508826.3 85.1% 94.4% (many diffs)
13 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508827.3 85.1% 94.4% (many diffs)
14 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508828.1 85.1% 94.4% (many diffs)
15 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508829.1 85.1% 94.4% (many diffs)
16 mouse 50767 Pnpla6 patatin-like phospholipase ... NM_001122818.2 84.8% 94% (many diffs)
17 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508831.3 84.8% 94% (many diffs)
18 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508822.3 84.4% 93.2% (many diffs)
19 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508832.3 84.4% 92.8% (many diffs)
20 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508824.3 84.1% 92.8% (many diffs)
21 mouse 50767 Pnpla6 patatin-like phospholipase ... XM_006508823.3 84% 92.4% (many diffs)
22 mouse 50767 Pnpla6 patatin-like phospholipase ... XR_378664.3 73.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
4047
ORF length:
3981
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ggctccgctg caaactggaa tggtgcttgg cgtgatgatc ggggccggag
121tggcggtggt ggtcacggcc gtgctcatcc tcctggtggt gcggaggctg cgagtgccaa
181aaaccccagc cccggatggc ccccggtatc ggttccggaa gagggacaaa gtgctcttct
241atggccggaa gattatgcgg aaggtgtcac aatccacctc ctccctcgtg gatacctctg
301tctccgccac ctcccggcca cgcatgagga agaaactgaa gatgctcaac attgccaaga
361agatcctgcg catccagaaa gagacgccca cgctgcagcg gaaggagccc ccgcccgcag
421tgctagaagc tgacctgacc gagggcgacc tggctaactc ccatctgccc tctgaagtgc
481tttatatgct caagaacgtc cgggtgctgg gccacttcga gaagccactc ttcctggagc
541tctgccgcca catggtcttc cagcggctgg gccagggtga ctacgtcttc cggccgggcc
601agccagatgc cagcatctac gtggtgcagg acgggctgct ggagctctgt ctgccagggc
661ctgacgggaa ggagtgtgtg gtgaaggaag tggttcctgg ggacagcgtc aacagccttc
721tcagcatcct ggatgtcatc accggtcacc agcatcccca gcggaccgtg tctgcccggg
781cggcccggga ctccacggtg ctgcgcctgc cggtggaagc attctccgcg gtcttcacca
841agtacccgga gagcttggtg cgggtcgtgc agatcatcat ggtgcggctg cagcgagtca
901ccttcctggc actgcacaac tacctgggtc tgaccaatga gctcttcagc cacgagatcc
961agcccctgcg tctgttcccc agccccggcc tcccaactcg caccagccct gtgcggggct
1021ccaagagaat ggtcagcacc tcagctacag acgagcccag ggagacccca gggcggccac
1081ccgatcccac cggggccccg ctgcctggac ctacagggga ccctgtgaag cccacatccc
1141tggaaacccc ctcgccccct ctgctgagcc gctgcgtctc catgccaggg gacatctcag
1201gcttgcaggg tggcccccgc tccgacttcg acatggccta tgagcgtggc cggatctccg
1261tgtccctgca agaagaggcc tccggggggt ccctggcagc ccccgctcgg acccccactc
1321aggagcctcg tgagcagccg gcaggcgcct gtgaatacag ctactgtgag gatgagtcgg
1381ccactggtgg ctgccctttc gggccctacc agggccgcca gaccagcagc atcttcgagg
1441cagcaaagca ggagctggcc aagctgatgc ggattgagga cccctccctc ctgaacagca
1501gagtcttgct gcaccacgcc aaagctggca ccatcattgc ccgccaggga gaccaggacg
1561tgagcctgca cttcgtgctc tggggctgcc tgcacgtgta ccagcgcatg atcgacaagg
1621cggaggacgt gtgcctgttc gtagcgcagc ccggggaact ggtggggcag ctggcggtgc
1681tcactggcga acctctcatc ttcacactgc gagcccaacg cgactgcacc ttcctgcgga
1741tctccaagtc cgacttctat gagatcatgc gcgcacagcc cagtgtggtg ctgagtgcgg
1801cgcacacggt ggcagccagg atgtcgccct tcgtgcgcca gatggacttc gccatcgact
1861ggactgcagt ggaggcggga cgcgcgctgt acaggcaggg cgaccgctcc gactgcactt
1921acatcgtgct caatgggcgg ctgcgtagcg tgatccagcg aggcagtggc aagaaggagc
1981tggtgggcga gtacggccgc ggcgacctca tcggcgtggt ggaggcactg acccggcagc
2041cgcgagccac gacggtgcac gcggtgcgcg acacggagct ggccaagctt cccgagggca
2101ccttgggtca catcaaacgc cggtacccgc aggtcgtgac ccgccttatc cacctactga
2161gccagaaaat tctagggaat ttgcagcagc tgcaaggacc cttcccagca ggctctgggt
2221tgggtgtgcc cccacactcg gaactcacca acccagccag caacctggca actgtggcaa
2281tcctgcctgt gtgtgctgag gtccccatgg tggccttcac gctggagctg cagcacgccc
2341tgcaggccat cggtccgacg ctactcctta acagtgacat catccgggca cgcctggggg
2401cctccgcact ggatagcatc caagagttcc ggctgtcagg gtggctggcc cagcaggagg
2461atgcacaccg tatcgtactc taccagacgg acgcctcgct gacgccctgg accgtgcgct
2521gcctgcgaca ggccgactgc atcctcattg tgggcctggg ggaccaggag cctaccctcg
2581gccagctgga gcagatgctg gagaacacgg ctgtgcgcgc ccttaagcag ctagtcctgc
2641tccaccgaga ggagggcgcg ggccccacgc gcaccgtgga gtggctaaat atgcgcagct
2701ggtgctcggg gcacctgcac ctgcgctgtc cgcgccgcct cttttcgcgc cgcagccctg
2761ccaagctgca tgagctctac gagaaggttt tctccaggcg cgcggaccgg cacagcgact
2821tctcccgctt ggcgagggtg ctcacgggga acaccattgc ccttgtgcta ggcgggggcg
2881gggccagggg ctgctcgcac atcggagtac taaaggcatt agaggaggcg ggggtccccg
2941tggacctggt gggcggcacg tccattggct ctttcatcgg agcgttgtac gcggaggagc
3001gcagcgccag ccgcacgagg cagcgggccc gggagtgggc caagagcatg acttcggtgc
3061tggaacctgt gttggacctc acgtacccag tcacctccat gttcactggg tctgccttta
3121accgcagcat ccatcgggtc ttccaggata agcagattga ggacctgtgg ctgccttact
3181tcaacgtgac cacagatatc accgcctcag ccatgcgagt ccacaaagat ggctccctgt
3241ggcggtacgt gcgcgccagc atgacgctgt cgggctacct gcccccgctg tgcgacccca
3301aggacgggca cctactcatg gatggcggct acatcaacaa tctgccagcg gacatcgccc
3361gcagcatggg tgccaaaacg gtcatcgcca ttgacgtggg gagccaggat gagacggacc
3421tcagcaccta cggggacagc ctgtccggct ggtggctgct gtggaagcgg ctgaatccct
3481gggctgacaa ggtaaaggtt ccagacatgg ctgaaatcca gtcccgcctg gcctacgtgt
3541cctgtgtgcg gcagctagag gttgtcaagt ccagctccta ctgcgagtac ctgcgcccgc
3601ccatcgactg cttcaagacc atggactttg ggaagttcga ccagatctat gatgtgggct
3661accagtacgg gaaggcggtg tttggaggct ggagccgtgg caacgtcatt gagaaaatgc
3721tcacagaccg gcggtctaca gaccttaatg agagccgccg tgcagacgtg cttgccttcc
3781caagctctgg cttcactgac ttggcagaga ttgtgtcccg gattgagccc cccacgagct
3841atgtctctga tggctgtgct gacggagagg agtcagattg tctgacagag tatgaggagg
3901acgccggacc cgactgctcg agggatgaag gggggtcccc cgagggcgca agccccagca
3961ctgcctccga gatggaggag gagaagtcga ttctccggca acgacgctgt ctgccccagg
4021agccgcccgg ctcagccaca gatgcctnnc caactttctt gtacaaagtg gttggtaagc
4081ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
4141ggcgcgttaa gtcgacaatc aacctctgga tta

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