Construct: ORF ccsbBroad304_15752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012638.1_s304c1, BRDN0000401831
- Derived from:
- ccsbBroadEn_15752
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCB1 (23236)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15752
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23236 | PLCB1 | phospholipase C beta 1 | NM_182734.3 | 99.9% | 100% | 2565G>A;2988T>C;3337C>T |
| 2 | human | 23236 | PLCB1 | phospholipase C beta 1 | NM_015192.4 | 95.3% | 94.4% | (many diffs) |
| 3 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | NM_019677.2 | 89.4% | 98% | (many diffs) |
| 4 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | NM_001145830.1 | 85.5% | 92.8% | (many diffs) |
| 5 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | XM_006498930.2 | 80.9% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3588
- ORF length:
- 3519
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccggggct caacccggag tgcacgcctt gcaactcaag cccgtgtgcg 121 tgtccgacag cctcaagaag ggcaccaaat tcgtcaagtg ggatgatgac tcaactattg 181 ttactccaat tattttgagg actgaccctc agggattttt cttttactgg acagatcaaa 241 acaaggagac agagctactg gatctcagcc ttgtcaaaga tgccagatgt gggagacacg 301 ccaaagctcc caaggacccc aaattacgtg aacttttgga tgtggggaac atcgggcgcc 361 tggagcagcg catgatcaca gtggtgtatg ggcctgacct cgtgaacatc tcccatttga 421 atctcgtggc tttccaagaa gaagtggcca aggaatggac aaatgaggtt ttcagtttgg 481 caacaaacct gctggcccaa aacatgtcca gggatgcatt tctggaaaaa gcctatacta 541 aacttaagct gcaagtcact ccagaagggc gtattcctct caaaaacata tatcgcttgt 601 tttcagcaga tcggaagcga gttgaaactg ctttagaggc ttgtagtctt ccatcttcaa 661 ggaatgattc aatacctcaa gaagatttca ctccagaagt gtacagagtt ttcctcaaca 721 acctttgccc tcgacctgaa attgataaca tcttttcaga atttggtgca aaaagcaaac 781 catatcttac cgttgatcag atgatggatt ttatcaacct taagcagcga gatcctcggc 841 ttaatgaaat actttatcca cctctaaaac aagagcaagt ccaagtattg attgagaagt 901 atgaacccaa caacagcctc gccagaaaag gacaaatatc agtggatggg ttcatgcgct 961 atctgagtgg agaagaaaac ggagtcgttt cacctgagaa actggatttg aatgaagaca 1021 tgtctcagcc cctttctcac tatttcatta attcctcgca caacacctac ctcacagctg 1081 gccaactggc tggaaactcc tctgttgaga tgtatcgcca agtgctcctg tctggttgtc 1141 gctgtgtgga gctggactgc tggaagggac ggactgcaga agaggaacct gtcatcaccc 1201 atggcttcac catgacaact gaaatatctt tcaaggaagt gatagaagca attgcggagt 1261 gtgcatttaa gacttcacct tttccaattc tcctttcgtt tgagaaccat gtggattccc 1321 caaagcagca agccaagatg gcggagtact gccgactgat ctttggggat gcccttctca 1381 tggagcccct ggaaaaatat ccactggaat ctggagttcc tcttccaagc cctatggatt 1441 taatgtataa aattttggtg aaaaataaga agaaatcaca caagtcatca gaaggaagcg 1501 gcaaaaagaa gctctcagaa caagcctcca acacctacag tgactcctcc agcatgttcg 1561 agccctcatc cccaggagcc ggagaagctg atacggaaag tgacgacgac gatgatgatg 1621 atgactgtaa aaaatcttca atggatgagg ggactgctgg aagtgaggct atggccacag 1681 aagaaatgtc taatctggtg aactatattc agccagtcaa gtttgagtca tttgaaattt 1741 caaaaaaaag aaataaaagt tttgaaatgt cttccttcgt ggaaaccaaa ggacttgaac 1801 aactcaccaa gtctccagtg gaatttgtag aatataacaa aatgcagctt agcaggatat 1861 atccaaaagg aacacgtgtg gattcatcca actatatgcc tcagctcttc tggaatgcag 1921 gttgtcagat ggtggcactt aatttccaga caatggacct ggctatgcaa ataaatatgg 1981 ggatgtatga atacaacggg aagagtggct acagattgaa gccagagttc atgaggaggc 2041 ctgacaagca ttttgatcca tttactgaag gcatcgtaga tgggatagtg gcaaacactt 2101 tgtctgttaa gattatttca ggtcagtttc tttctgataa gaaagttggg acttacgtgg 2161 aagtagatat gtttggtttg cctgtggata caaggaggaa ggcatttaag accaaaacat 2221 cccaaggaaa tgctgtgaat cctgtctggg aagaagaacc tattgtgttc aaaaaggtgg 2281 ttcttcctac tctggcctgt ttgagaatag cagtttatga agaaggaggt aaattcattg 2341 gccaccgtat cttgccagtg caagccattc ggccaggcta tcactatatc tgtctaagga 2401 atgaaaggaa ccagcctctg acgctgcctg ctgtctttgt ctacatagaa gtgaaagact 2461 atgtgccaga cacatatgca gatgtcatcg aagctttatc aaacccaatc cgatatgtga 2521 acctgatgga acagagagct aagcaattgg ctgctttgac actggaagat gaagaagaag 2581 taaagaaaga ggctgatcct ggagaaacac catcagaggc tccaagtgaa gcaagaacga 2641 ctccagcaga aaatggggtg aatcacacta caaccctgac acccaagcca ccctcccagg 2701 ctctccacag ccagccagct ccaggttctg taaaggcacc tgccaaaaca gaagatctta 2761 ttcagagtgt cttaacagaa gtggaagcac agaccatcga agaactaaag caacagaaat 2821 cgtttgtgaa acttcaaaag aaacactaca aagaaatgaa agacctggtt aagagacacc 2881 acaagaaaac cactgacctt atcaaagaac acactaccaa gtataatgaa attcagaatg 2941 actacttgag aaggagagcc gctttggaaa agtccgccaa aaaggacagt aagaaaaaat 3001 cggaacccag cagccctgat catggttcat caacgattga gcaagacctc gctgccctgg 3061 atgctgaaat gacccaaaag ttaatagact tgaaggacaa acaacagcag cagctgctta 3121 atcttcggca agaacagtat tatagtgaaa aataccagaa gcgagaacat attaaactgc 3181 ttattcaaaa gttgacggat gtcgcagaag agtgtcagaa caatcagtta aagaagctca 3241 aagaaatctg tgagaaagaa aagaaagaat taaagaagaa aatggataaa aagaggcagg 3301 agaagataac agaagctaaa tccaaagaca aaagtcagat ggaagaggag aagacagaga 3361 tgatccggtc atatatccag gaagtggtgc agtatatcaa gaggttagaa gaagcgcaaa 3421 gtaaacggca agaaaaactc gtagagaaac acaaggaaat acgtcagcag atcctggatg 3481 aaaagcccaa gggggaaggt tcctcctcat tcttgtcgga aacttgccat gaggatccct 3541 ctgtttcccc caactttact ccccccaacc ctcaagctct caagtggttg ccaactttct 3601 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 3661 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta