Construct: ORF ccsbBroad304_15765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012581.1_s304c1, BRDN0000399690
- Derived from:
- ccsbBroadEn_15765
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NNT (23530)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15765
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_005248275.5 | 35.2% | 35.3% | 1_1137del;1398A>G |
| 2 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_024446009.1 | 35.2% | 35.3% | 1_1137del;1398A>G |
| 3 | human | 23530 | NNT | nicotinamide nucleotide tra... | NM_001331026.2 | 21.6% | 21.6% | 1_2244del;2505A>G |
| 4 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_006714461.4 | 21.6% | 21.6% | 1_2244del;2505A>G |
| 5 | human | 23530 | NNT | nicotinamide nucleotide tra... | NM_012343.4 | 19% | 19% | 1_2637del;2898A>G |
| 6 | human | 23530 | NNT | nicotinamide nucleotide tra... | NM_182977.3 | 19% | 19% | 1_2637del;2898A>G |
| 7 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_005248274.5 | 19% | 19% | 1_2637del;2898A>G |
| 8 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_011514001.3 | 19% | 19% | 1_2637del;2898A>G |
| 9 | human | 23530 | NNT | nicotinamide nucleotide tra... | XM_017009293.2 | 19% | 19% | 1_2637del;2898A>G |
| 10 | mouse | 18115 | Nnt | nicotinamide nucleotide tra... | NM_001308506.1 | 22% | 24.3% | (many diffs) |
| 11 | mouse | 18115 | Nnt | nicotinamide nucleotide tra... | NM_008710.3 | 16.9% | 18.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcgctccctg gctaatgtga ttcttggagg ctatggcacc acttcaacag 121 ctggtggaaa acccatggaa atttctggca cacatacgga aatcaacctt gacaatgcaa 181 ttgacatgat tcgagaagct aatagcatta ttattacacc aggctatggt ctctgtgcag 241 ccaaagctca ataccccatt gctgatttgg taaagatgct cactgagcaa ggcaaaaaag 301 tcaggtttgg aattcaccca gttgcgggcc gaatgcctgg tcagcttaat gtgctgctgg 361 ctgaggctgg tgtgccatat gacattgtgt tggaaatgga tgagatcaac catgattttc 421 cagatactga tttggtcctt gtaattggag ctaatgacac tgttaattca gcagctcaag 481 aagatcccaa ctctattatt gcaggcatgc cagtccttga ggtctggaaa tcaaagcagg 541 tgattgttat gaagaggtct ttgggtgttg gctatgctgc agtggacaat ccaatcttct 601 acaaacctaa cacggccatg cttctaggtg atgccaagaa aacatgtgac gcgctccagg 661 cgaaagttag agaatcctat cagaagtnnc caactttctt gtacaaagtg gttggtaagc 721 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 781 ggcgcgttaa gtcgacaatc aacctctgga tta