Construct: ORF ccsbBroad304_15801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006364.1_s304c1, BRDN0000407896
- Derived from:
- ccsbBroadEn_15801
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFT81 (28981)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15801
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_001347947.2 | 29.2% | 29.2% | 1_777del |
| 2 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_001347948.2 | 29.2% | 29.2% | 1_777del |
| 3 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_001143779.2 | 15.8% | 15.8% | 1_1707del |
| 4 | human | 28981 | IFT81 | intraflagellar transport 81 | NM_014055.4 | 15.8% | 15.8% | 1_1707del |
| 5 | human | 28981 | IFT81 | intraflagellar transport 81 | XM_017019217.1 | 15.8% | 15.8% | 1_1707del |
| 6 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144950.2 | 12% | 1_1773del;2095_2664del | |
| 7 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_429093.3 | 11.8% | 1_1820del;2142_2711del | |
| 8 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144951.2 | 11.3% | 1_1937del;2259_2828del | |
| 9 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144949.2 | 10.1% | 1_2265del;2587_3156del | |
| 10 | human | 28981 | IFT81 | intraflagellar transport 81 | XR_001748672.2 | 10% | 1_2312del;2634_3203del | |
| 11 | human | 28981 | IFT81 | intraflagellar transport 81 | NR_144952.2 | 9.8% | 1_2355del;2677_3246del | |
| 12 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_006530129.3 | 15.4% | 16.2% | (many diffs) |
| 13 | mouse | 12589 | Ift81 | intraflagellar transport 81 | NM_009879.3 | 13.8% | 14.4% | (many diffs) |
| 14 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_006530127.3 | 13.8% | 14.4% | (many diffs) |
| 15 | mouse | 12589 | Ift81 | intraflagellar transport 81 | XM_011248162.1 | 13.1% | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 387
- ORF length:
- 321
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat taagaaccta gaagttcaac ttcgtcgtgc tactgatgag atgaaggcat 121 atatctcttc tgatcaacaa gaaaaaagaa aggcaattag ggaacagtat accaaaaata 181 ctgctgaaca agaaaacctt ggaaagaaac ttcgggaaaa acaaaaagtt atacgagaaa 241 gtcatggtcc aaatatgaaa caagcaaaaa tgtggcgtga tttggaacaa ttaatggaat 301 gtaagaaaca gtgctttctg aaacaacaaa gccaaacttc cattggtcag gtaattcagg 361 agggtgggga ggaccggcta atactgtnnc caactttctt gtacaaagtg gttggtaagc 421 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 481 ggcgcgttaa gtcgacaatc aacctctgga tta