Construct: ORF ccsbBroad304_15871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011030.1_s304c1, BRDN0000407695
- Derived from:
- ccsbBroadEn_15871
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL28 (54813)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15871
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54813 | KLHL28 | kelch like family member 28 | NM_017658.5 | 88.9% | 88.2% | 15C>A;1508_1509delAA;1527_1713del |
| 2 | human | 54813 | KLHL28 | kelch like family member 28 | XM_005267770.4 | 88.9% | 88.2% | 15C>A;1508_1509delAA;1527_1713del |
| 3 | human | 54813 | KLHL28 | kelch like family member 28 | XM_011536847.3 | 88.9% | 88.2% | 15C>A;1508_1509delAA;1527_1713del |
| 4 | human | 54813 | KLHL28 | kelch like family member 28 | XM_024449635.1 | 88.6% | 87.5% | (many diffs) |
| 5 | human | 54813 | KLHL28 | kelch like family member 28 | NM_001308112.2 | 86.7% | 86.1% | (many diffs) |
| 6 | human | 54813 | KLHL28 | kelch like family member 28 | XM_011536849.2 | 86.3% | 85.1% | (many diffs) |
| 7 | human | 54813 | KLHL28 | kelch like family member 28 | XM_017021378.1 | 60.2% | 59.4% | (many diffs) |
| 8 | human | 54813 | KLHL28 | kelch like family member 28 | XM_006720174.3 | 58.6% | 57.8% | (many diffs) |
| 9 | mouse | 66689 | Klhl28 | kelch-like 28 | XM_006516157.3 | 84% | 88.7% | (many diffs) |
| 10 | mouse | 66689 | Klhl28 | kelch-like 28 | NM_025707.3 | 80.8% | 85.2% | (many diffs) |
| 11 | mouse | 66689 | Klhl28 | kelch-like 28 | XM_006516156.3 | 80.8% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ccacacatca ccgacctaca tgcttgctaa cttaacccac ttgcattctg 121 aacaacttct gcagggcttg aatcttcttc gccaacatca cgaactctgt gacatcattc 181 ttcgagtagg tgatgttaaa attcatgctc acaaagtggt acttgccagc gtcagcccgt 241 atttcaaagc tatgttcact ggaaaccttt ctgaaaaaga gaacagtgag gttgagtttc 301 aatgcattga tgaaactgct ctccaggcca ttgtggagta tgcctataca gggactgttt 361 ttatttctca ggacacagtt gaatctctcc tgccagcagc aaacctactc cagataaaac 421 ttgtcctgaa agaatgttgt gcatttcttg aaagccaact tgatcctggt aattgtattg 481 gaatttctcg ttttgcagaa acatatggtt gccgtgacct ttatttggca gccactaaat 541 acatatgcca gaattttgaa gctgtttgcc agactgaaga gttttttgag cttacacatg 601 ctgacttgga tgaaattgtt tccaatgact gtttgaatgt agctaccgaa gagactgttt 661 tttatgcatt agagtcttgg atcaagtatg atgtacaaga acgccagaaa tacttagcac 721 agttactaaa cagtgtacga ttaccattgt tgagtgttaa gtttctcact agactatatg 781 aagcaaatca tcttattcgt gatgatcgca cttgtaaaca tcttttgaat gaagccctaa 841 agtaccactt tatgcctgaa catagactct ctcatcagac agtcttgatg acacgacctc 901 gctgtgctcc caaagtactt tgtgcagtag gagggaaatc tggactcttt gcctgtttgg 961 atagtgtgga gatgtacttt cctcagaatg actcttggat tggtttggca cccctaaaca 1021 ttcctcgcta tgaatttgga atatgcgttt tagaccaaaa agtatatgtt ataggtggta 1081 ttgcaactaa tgtgcgtcct ggcgtcacta tcagaaaaca tgaaaattca gtggaatgct 1141 ggaatcctga tacaaatact tggacttctc tagagagaat gaatgaaagc cgaagtactc 1201 ttggagtagt agtacttgca ggagaacttt atgccttagg tggttatgat ggacaatctt 1261 atttacaatc tgtagagaag tacattccca aaataagaaa atggcaacct gtggcaccaa 1321 tgacgacaac aagaagttgt tttgctgcag cggtattgga tggaatgata tatgccattg 1381 gtgggtatgg tcctgcccac atgaacagtg tggagcgtta tgatccaagt aaggactcct 1441 gggagatggt tgcatccatg gcagataaaa ggattcactt tggcgtgggt gtcatgctag 1501 gctttatttt tgtggtgggt ggacataatg gagtctcaca tttgtccagc attgaaagat 1561 acgatcctca tcaatcagtg gactgtgtgt nnccaacttt cttgtacaaa gtggttggta 1621 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1681 ggtggcgcgt taagtcgaca atcaacctct ggatta