Construct: ORF ccsbBroad304_15919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012613.1_s304c1, BRDN0000402565
- Derived from:
- ccsbBroadEn_15919
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB5IF (55969)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15919
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_001374178.1 | 65.4% | 57.3% | (many diffs) |
| 2 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_018840.5 | 61.4% | 54% | (many diffs) |
| 3 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_001199534.2 | 57.9% | 58.3% | (many diffs) |
| 4 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_199483.3 | 56.8% | 51.7% | (many diffs) |
| 5 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_164350.1 | 24.8% | 1_173del;435_1050del | |
| 6 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_026562.4 | 22.1% | 1_173del;435_1180del | |
| 7 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_164351.1 | 21.7% | 1_173del;391_411del;456_1201del | |
| 8 | mouse | 67388 | 1110008F13Rik | RIKEN cDNA 1110008F13 gene | NM_026124.3 | 57.6% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 327
- ORF length:
- 261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcgggcgg cggaaggagg agccgcctca gccgcagctg gccaacgggg 121 ccctcaaagt ctccgtctgg agtaaggtgc tgcggagcga cgcggcctgg gaggataagg 181 atgaattttt agatgtgatc tactggttcc gacagatcat tgctgtggtc ctgggtgtca 241 tttggggagt tttgccatta cgagggttct tgggaatagc agggaaaggc cattgctgct 301 ctggagctgt gtgtgtgtgt gatgactnnc caactttctt gtacaaagtg gttggtaagc 361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 421 ggcgcgttaa gtcgacaatc aacctctgga tta