Construct: ORF ccsbBroad304_15942
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005666.1_s304c1, BRDN0000405029
- Derived from:
- ccsbBroadEn_15942
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELI1 (57162)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15942
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532997.2 | 52.3% | 52.3% | 1_342del |
| 2 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_005264437.3 | 39% | 39% | 1_585del |
| 3 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532996.2 | 39% | 39% | 1_585del |
| 4 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004521.2 | 35.5% | 35.5% | 1_681del |
| 5 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | NM_020651.4 | 29.9% | 29.9% | 1_879del |
| 6 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532994.3 | 29.9% | 29.9% | 1_879del |
| 7 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004520.1 | 29.9% | 29.9% | 1_879del |
| 8 | mouse | 67245 | Peli1 | pellino 1 | XM_011243746.1 | 33.4% | 35.5% | (many diffs) |
| 9 | mouse | 67245 | Peli1 | pellino 1 | NM_023324.2 | 28.1% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaggaaagac gttgtagatg aaaaacaacc atgggtatat ctaaactgcg 121 gccatgtaca tggctatcat aactggggaa acaaagaaga acgtgatgga aaagatcgtg 181 aatgtcctat gtgtaggtct gttggtccct atgttcctct gtggcttgga tgtgaagctg 241 gattttatgt ggacgccggc cctccaaccc atgcgtttag cccgtgtggg catgtgtgtt 301 cagaaaagac aactgcctat tggtcccaga tcccacttcc tcatggtact catacttttc 361 atgcagcctg tcccttttgt gcacatcagt tggctggtga acaaggctac atcagactta 421 tttttcaagg acctctagac tnnccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta