Construct: ORF ccsbBroad304_15977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015107.1_s304c1, BRDN0000406084
- Derived from:
- ccsbBroadEn_15977
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF655 (79027)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15977
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001085367.1 | 100% | 100% | |
| 2 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_024061.3 | 100% | 100% | |
| 3 | human | 79027 | ZNF655 | zinc finger protein 655 | XM_017012611.2 | 72.6% | 62.6% | (many diffs) |
| 4 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001009958.1 | 60.7% | 60.7% | 134_135ins213 |
| 5 | human | 79027 | ZNF655 | zinc finger protein 655 | NM_001085366.1 | 60.7% | 60.7% | 134_135ins213 |
| 6 | mouse | 72611 | Zfp655 | zinc finger protein 655 | NM_001083958.1 | 91.5% | 85.6% | (many diffs) |
| 7 | mouse | 72611 | Zfp655 | zinc finger protein 655 | NM_001347533.1 | 53.9% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaaatacca gcccaggaag cagcagggtc accaagggtc cagtttcagt 121 ctttggagac ccagtctgag tgtctgtccc cagagcctca gtttgtgcag gacaccgaca 181 tggaacaggg actcactggg gctccacctg ttcctcaggt gcctgctctt ccccgtgagg 241 gaagcccagg agaccaggca gctgcgctct tgacagccag gtaccaggag tttgtgacat 301 tcgaggatgt ggctgtgcac cttactcgag aggaatgggg atacctggac cctgttcaga 361 gggacctcta cagagaagtg atgttagaga attatgggaa cgtggtctca ctgggcatac 421 ttctccgcct tcccaccacc cggattcata gtgtgaattc ctgcccggcc ctgagtcata 481 cccaggcaag tgctttctct ggagaaacac ttgccgtcct tacagcagga atctccaaga 541 gatggcccaa gtatcggctt cccatcgata ttgctcgtcc ctgctcggaa actccttttc 601 cacgattgtn nccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 661 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 721 tcaacctctg gatta