Construct: ORF ccsbBroad304_16077

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012127.1_s304c1, BRDN0000404524
Derived from:
ccsbBroadEn_16077
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL32 (114792)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16077
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114792 KLHL32 kelch like family member 32 NM_001323258.1 66% 65.4% 413_433del;438_630delinsG
2 human 114792 KLHL32 kelch like family member 32 NM_001323259.2 66% 65.4% 413_433del;438_630delinsG
3 human 114792 KLHL32 kelch like family member 32 NM_001323256.2 49.5% 49.1% 413_433del;438_840delinsG
4 human 114792 KLHL32 kelch like family member 32 NM_001323255.2 34.8% 34.4% 412_417delCTAGCT;419_420insG;423_1194del
5 human 114792 KLHL32 kelch like family member 32 NM_001323254.1 30.6% 30.3% 412_417delCTAGCT;419_420insG;423_1356del
6 human 114792 KLHL32 kelch like family member 32 NM_001286252.2 29.5% 29.2% 413_433del;438_1410delinsG
7 human 114792 KLHL32 kelch like family member 32 NM_001323253.1 26.4% 26.2% 413_433del;438_1572delinsG
8 human 114792 KLHL32 kelch like family member 32 NM_001323257.1 25.2% 25% 412_417delCTAGCT;419_420insG;423_1644del
9 human 114792 KLHL32 kelch like family member 32 NM_001323252.1 22.3% 22.2% 413_433del;438_1860delinsG
10 human 114792 KLHL32 kelch like family member 32 NM_052904.4 22.3% 22.2% 413_433del;438_1860delinsG
11 human 114792 KLHL32 kelch like family member 32 NM_001286250.1 16.5% 16.4% 203_204ins108;305_325del;330_1752delinsG
12 human 114792 KLHL32 kelch like family member 32 NR_104421.1 14.5% 1_472del;890_2864del
13 human 114792 KLHL32 kelch like family member 32 NR_136580.1 13.5% 1_472del;885_905del;910_3080delinsG
14 human 114792 KLHL32 kelch like family member 32 NM_001323264.1 13.3% 12.5% (many diffs)
15 human 114792 KLHL32 kelch like family member 32 XM_017010228.2 12.7% 12.4% (many diffs)
16 human 114792 KLHL32 kelch like family member 32 NM_001323263.1 11.8% 11.2% (many diffs)
17 human 114792 KLHL32 kelch like family member 32 XM_005266813.5 11.2% 11.1% 0_1ins207;206_226del;231_1653delinsG
18 human 114792 KLHL32 kelch like family member 32 XR_001743153.2 3.3% (many diffs)
19 mouse 212390 Klhl32 kelch-like 32 XM_017320094.2 51.2% 53.4% (many diffs)
20 mouse 212390 Klhl32 kelch-like 32 NM_001163020.2 20.4% 21.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
483
ORF length:
417
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gtctgaacgc tgcctcagta ttcaagaaat gctgacaggc cagaggctct
121gccactccga atctcacaat gacagtgtcc tggcagcgct gaatcagcag aggagtgatg
181gcatcctctg cgacatcacc ctgattgctg aggaacagaa attccatgct cacaaggcag
241tcctagcagc atgcagtgac tatttccggg caatgttcag tctttgtatg gtggaaagtg
301gagctgatga ggttaatttg cacggtgtga ccagccttgg cttaaagcag gctctggagt
361ttgcatacac aggacagatt ttgctggagc caggtgtgat ccaggatgtg ctagcagcgg
421gcagtcacct acagctgttg gagcttctca atttatgctc ccactatctc atccaggtgg
481agtnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
541tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
601tctggatta

Download FASTA (ORF) (Full)

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