Construct: ORF ccsbBroad304_16077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012127.1_s304c1, BRDN0000404524
- Derived from:
- ccsbBroadEn_16077
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL32 (114792)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16077
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323258.1 | 66% | 65.4% | 413_433del;438_630delinsG |
| 2 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323259.2 | 66% | 65.4% | 413_433del;438_630delinsG |
| 3 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323256.2 | 49.5% | 49.1% | 413_433del;438_840delinsG |
| 4 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323255.2 | 34.8% | 34.4% | 412_417delCTAGCT;419_420insG;423_1194del |
| 5 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323254.1 | 30.6% | 30.3% | 412_417delCTAGCT;419_420insG;423_1356del |
| 6 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001286252.2 | 29.5% | 29.2% | 413_433del;438_1410delinsG |
| 7 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323253.1 | 26.4% | 26.2% | 413_433del;438_1572delinsG |
| 8 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323257.1 | 25.2% | 25% | 412_417delCTAGCT;419_420insG;423_1644del |
| 9 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323252.1 | 22.3% | 22.2% | 413_433del;438_1860delinsG |
| 10 | human | 114792 | KLHL32 | kelch like family member 32 | NM_052904.4 | 22.3% | 22.2% | 413_433del;438_1860delinsG |
| 11 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001286250.1 | 16.5% | 16.4% | 203_204ins108;305_325del;330_1752delinsG |
| 12 | human | 114792 | KLHL32 | kelch like family member 32 | NR_104421.1 | 14.5% | 1_472del;890_2864del | |
| 13 | human | 114792 | KLHL32 | kelch like family member 32 | NR_136580.1 | 13.5% | 1_472del;885_905del;910_3080delinsG | |
| 14 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323264.1 | 13.3% | 12.5% | (many diffs) |
| 15 | human | 114792 | KLHL32 | kelch like family member 32 | XM_017010228.2 | 12.7% | 12.4% | (many diffs) |
| 16 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323263.1 | 11.8% | 11.2% | (many diffs) |
| 17 | human | 114792 | KLHL32 | kelch like family member 32 | XM_005266813.5 | 11.2% | 11.1% | 0_1ins207;206_226del;231_1653delinsG |
| 18 | human | 114792 | KLHL32 | kelch like family member 32 | XR_001743153.2 | 3.3% | (many diffs) | |
| 19 | mouse | 212390 | Klhl32 | kelch-like 32 | XM_017320094.2 | 51.2% | 53.4% | (many diffs) |
| 20 | mouse | 212390 | Klhl32 | kelch-like 32 | NM_001163020.2 | 20.4% | 21.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtctgaacgc tgcctcagta ttcaagaaat gctgacaggc cagaggctct 121 gccactccga atctcacaat gacagtgtcc tggcagcgct gaatcagcag aggagtgatg 181 gcatcctctg cgacatcacc ctgattgctg aggaacagaa attccatgct cacaaggcag 241 tcctagcagc atgcagtgac tatttccggg caatgttcag tctttgtatg gtggaaagtg 301 gagctgatga ggttaatttg cacggtgtga ccagccttgg cttaaagcag gctctggagt 361 ttgcatacac aggacagatt ttgctggagc caggtgtgat ccaggatgtg ctagcagcgg 421 gcagtcacct acagctgttg gagcttctca atttatgctc ccactatctc atccaggtgg 481 agtnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 541 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 601 tctggatta