Transcript: Human NM_001323257.1

Homo sapiens kelch like family member 32 (KLHL32), transcript variant 12, mRNA.

Source:
NCBI, updated 2019-01-12
Taxon:
Homo sapiens (human)
Gene:
KLHL32 (114792)
Length:
3591
CDS:
473..2119

Additional Resources:

NCBI RefSeq record:
NM_001323257.1
NBCI Gene record:
KLHL32 (114792)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001323257.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000267431 TACATCCGCTTTGGCCTAATG pLKO_005 944 CDS 100% 10.800 8.640 N Klhl32 n/a
2 TRCN0000145334 GCTTCCTTGTCTTGCTTTATA pLKO.1 2199 3UTR 100% 15.000 10.500 N KLHL32 n/a
3 TRCN0000122442 GCTGTCCATAATGGGAGAATA pLKO.1 1883 CDS 100% 13.200 9.240 N KLHL32 n/a
4 TRCN0000121810 GCGAACACTTTGTAATACTTT pLKO.1 2816 3UTR 100% 5.625 3.938 N KLHL32 n/a
5 TRCN0000142744 CGGTACTTCAATCCTGTTGAT pLKO.1 1178 CDS 100% 4.950 3.465 N KLHL32 n/a
6 TRCN0000143709 GCATGTGAAAGAGACACACAA pLKO.1 2986 3UTR 100% 4.950 3.465 N KLHL32 n/a
7 TRCN0000145448 GCTAATGGTGTATGAACCTAA pLKO.1 1645 CDS 100% 4.950 3.465 N KLHL32 n/a
8 TRCN0000122464 GCTGAGGAACAGAAATTCCAT pLKO.1 614 CDS 100% 3.000 1.800 N KLHL32 n/a
9 TRCN0000166364 CACACACACACACACACACAA pLKO.1 183 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001323257.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13037 pDONR223 100% 62.4% 61.4% None (many diffs) n/a
2 ccsbBroad304_13037 pLX_304 0% 62.4% 61.4% V5 (many diffs) n/a
3 TRCN0000471330 GTTTAGTAACCGAACCCGCTACAT pLX_317 28.1% 62.4% 61.4% V5 (many diffs) n/a
4 ccsbBroadEn_16077 pDONR223 0% 25.2% 25% None 412_417delCTAGCT;419_420insG;423_1644del n/a
5 ccsbBroad304_16077 pLX_304 0% 25.2% 25% V5 412_417delCTAGCT;419_420insG;423_1644del n/a
6 TRCN0000475586 AGCCTCTCCTTGCTATCGAGCAAG pLX_317 70.9% 25.2% 25% V5 412_417delCTAGCT;419_420insG;423_1644del n/a
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