Transcript: Human NM_001323255.2

Homo sapiens kelch like family member 32 (KLHL32), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
KLHL32 (114792)
Length:
1736
CDS:
365..1561

Additional Resources:

NCBI RefSeq record:
NM_001323255.2
NBCI Gene record:
KLHL32 (114792)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001323255.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000267431 TACATCCGCTTTGGCCTAATG pLKO_005 836 CDS 100% 10.800 8.640 N Klhl32 n/a
2 TRCN0000142744 CGGTACTTCAATCCTGTTGAT pLKO.1 1070 CDS 100% 4.950 3.465 N KLHL32 n/a
3 TRCN0000122464 GCTGAGGAACAGAAATTCCAT pLKO.1 506 CDS 100% 3.000 1.800 N KLHL32 n/a
4 TRCN0000166364 CACACACACACACACACACAA pLKO.1 75 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001323255.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13037 pDONR223 100% 84.6% 84.6% None 408_409ins216 n/a
2 ccsbBroad304_13037 pLX_304 0% 84.6% 84.6% V5 408_409ins216 n/a
3 TRCN0000471330 GTTTAGTAACCGAACCCGCTACAT pLX_317 28.1% 84.6% 84.6% V5 408_409ins216 n/a
4 ccsbBroadEn_16077 pDONR223 0% 34.8% 34.4% None 412_417delCTAGCT;419_420insG;423_1194del n/a
5 ccsbBroad304_16077 pLX_304 0% 34.8% 34.4% V5 412_417delCTAGCT;419_420insG;423_1194del n/a
6 TRCN0000475586 AGCCTCTCCTTGCTATCGAGCAAG pLX_317 70.9% 34.8% 34.4% V5 412_417delCTAGCT;419_420insG;423_1194del n/a
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