Construct: ORF ccsbBroad304_16160

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004996.1_s304c1, BRDN0000402053
Derived from:
ccsbBroadEn_16160
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PKD1P1 (339044)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16160
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100288332 NPIPA5 nuclear pore complex intera... NM_001351200.1 100% 100%
2 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452522.1 99.8% 99.5% 277C>T
3 human 642778 NPIPA3 nuclear pore complex intera... XM_005255485.4 99.5% 99.1% 277C>T;364T>C;510C>T
4 human 642799 NPIPA2 nuclear pore complex intera... XM_005255492.4 99.5% 99.1% 277C>T;364T>C;510C>T
5 human 101059938 NPIPA7 nuclear pore complex intera... XM_024450138.1 99.2% 98.2% (many diffs)
6 human 101059938 NPIPA7 nuclear pore complex intera... XM_024450137.1 90.6% 87.2% (many diffs)
7 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030213.2 85.6% 81.1% (many diffs)
8 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452521.1 81% 73.6% (many diffs)
9 human 100288332 NPIPA5 nuclear pore complex intera... XM_011522336.3 80% 77.7% (many diffs)
10 human 642799 NPIPA2 nuclear pore complex intera... XM_006720917.4 79.7% 76.4% (many diffs)
11 human 642778 NPIPA3 nuclear pore complex intera... XM_011522581.1 79.7% 76.4% (many diffs)
12 human 642778 NPIPA3 nuclear pore complex intera... XM_011522583.3 79.7% 76.4% (many diffs)
13 human 642799 NPIPA2 nuclear pore complex intera... XM_011522593.1 79.7% 76.4% (many diffs)
14 human 642799 NPIPA2 nuclear pore complex intera... XM_011522594.3 79.7% 76.4% (many diffs)
15 human 101059938 NPIPA7 nuclear pore complex intera... XM_024450139.1 76.3% 72.9% (many diffs)
16 human 642778 NPIPA3 nuclear pore complex intera... XM_011522584.2 75.4% 69.3% (many diffs)
17 human 642799 NPIPA2 nuclear pore complex intera... XM_011522595.2 75.4% 69.3% (many diffs)
18 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756353.1 68.7% (many diffs)
19 human 100288332 NPIPA5 nuclear pore complex intera... NM_001277325.2 64.4% 61.2% (many diffs)
20 human 9284 NPIPA1 nuclear pore complex intera... NM_006985.4 64.3% 61% (many diffs)
21 human 642778 NPIPA3 nuclear pore complex intera... NM_001277323.1 64.2% 60.4% (many diffs)
22 human 642799 NPIPA2 nuclear pore complex intera... NM_001277324.1 64.2% 60.4% (many diffs)
23 human 729978 NPIPB2 nuclear pore complex intera... XM_024450430.1 63.4% 53.1% (many diffs)
24 human 100288332 NPIPA5 nuclear pore complex intera... XM_011522329.3 61.7% 58.7% (many diffs)
25 human 642799 NPIPA2 nuclear pore complex intera... XM_011522587.1 61.5% 57.5% (many diffs)
26 human 642778 NPIPA3 nuclear pore complex intera... XM_024450380.1 61.5% 57.5% (many diffs)
27 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452519.1 61% 57.9% (many diffs)
28 human 642799 NPIPA2 nuclear pore complex intera... XM_024450387.1 61% 55% (many diffs)
29 human 101059938 NPIPA7 nuclear pore complex intera... NM_001282507.1 60.7% 57.1% (many diffs)
30 human 101059953 NPIPA8 nuclear pore complex intera... NM_001282511.2 60.7% 57.1% (many diffs)
31 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022834.1 60.7% 57.1% (many diffs)
32 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022835.1 60.7% 57.1% (many diffs)
33 human 100288332 NPIPA5 nuclear pore complex intera... XM_024450132.1 59% 51.9% (many diffs)
34 human 642778 NPIPA3 nuclear pore complex intera... XM_017023554.1 58.8% 51.1% (many diffs)
35 human 642778 NPIPA3 nuclear pore complex intera... XM_024450379.1 58.8% 51.1% (many diffs)
36 human 642799 NPIPA2 nuclear pore complex intera... XM_024450385.1 58.8% 51.1% (many diffs)
37 human 642799 NPIPA2 nuclear pore complex intera... XM_024450386.1 58.8% 51.1% (many diffs)
38 human 642778 NPIPA3 nuclear pore complex intera... XM_011522580.2 58.4% 55.2% (many diffs)
39 human 642799 NPIPA2 nuclear pore complex intera... XM_024450388.1 58.4% 55.2% (many diffs)
40 human 642778 NPIPA3 nuclear pore complex intera... XM_011522578.2 57.3% 51.1% (many diffs)
41 human 642799 NPIPA2 nuclear pore complex intera... XM_024450384.1 57.3% 51.1% (many diffs)
42 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022833.2 56.6% 51.4% (many diffs)
43 human 729978 NPIPB2 nuclear pore complex intera... XM_024450427.1 56.1% 44.7% (many diffs)
44 human 100288332 NPIPA5 nuclear pore complex intera... XM_011522337.1 55.8% 54.2% (many diffs)
45 human 101059938 NPIPA7 nuclear pore complex intera... XM_011522339.3 55.6% 52.5% (many diffs)
46 human 101059938 NPIPA7 nuclear pore complex intera... XM_024450136.1 55.6% 53.9% (many diffs)
47 human 642778 NPIPA3 nuclear pore complex intera... XM_011522585.1 55.3% 53.6% (many diffs)
48 human 642799 NPIPA2 nuclear pore complex intera... XM_011522596.1 55.3% 53.6% (many diffs)
49 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030211.2 54.7% 49.2% (many diffs)
50 human 642778 NPIPA3 nuclear pore complex intera... XM_011522576.2 54.6% 49.2% (many diffs)
51 human 642778 NPIPA3 nuclear pore complex intera... XM_011522577.2 54.6% 49.2% (many diffs)
52 human 642778 NPIPA3 nuclear pore complex intera... XM_017023553.1 54.6% 49.2% (many diffs)
53 human 642799 NPIPA2 nuclear pore complex intera... XM_024450381.1 54.6% 49.2% (many diffs)
54 human 642799 NPIPA2 nuclear pore complex intera... XM_024450382.1 54.6% 49.2% (many diffs)
55 human 642799 NPIPA2 nuclear pore complex intera... XM_024450383.1 54.6% 49.2% (many diffs)
56 human 101059938 NPIPA7 nuclear pore complex intera... XM_005255735.5 54.5% 48.7% (many diffs)
57 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452520.1 54.3% 49.2% (many diffs)
58 human 728888 NPIPB11 nuclear pore complex intera... XM_024450422.1 53.8% 41.4% (many diffs)
59 human 100288332 NPIPA5 nuclear pore complex intera... XR_002957772.1 51.8% (many diffs)
60 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756352.1 46.4% (many diffs)
61 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452523.1 41.5% 44% (many diffs)
62 human 728741 NPIPB6 nuclear pore complex intera... XM_011545931.2 36.4% 27.1% (many diffs)
63 human 728741 NPIPB6 nuclear pore complex intera... XM_005255741.4 36.2% 27.3% (many diffs)
64 human 728741 NPIPB6 nuclear pore complex intera... XM_017023630.1 36.2% 27.3% (many diffs)
65 human 728741 NPIPB6 nuclear pore complex intera... XM_024450419.1 36.2% 27.3% (many diffs)
66 human 728734 NPIPB8 nuclear pore complex intera... XM_024450417.1 35.9% 26.7% (many diffs)
67 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022825.2 35.6% 26.9% (many diffs)
68 human 728734 NPIPB8 nuclear pore complex intera... XM_017023626.1 35.6% 26.9% (many diffs)
69 human 728734 NPIPB8 nuclear pore complex intera... XM_024450416.1 35.6% 26.9% (many diffs)
70 human 100288332 NPIPA5 nuclear pore complex intera... XR_932754.3 34.7% (many diffs)
71 human 440348 NPIPB15 nuclear pore complex intera... NM_001306094.1 33.6% 25.5% (many diffs)
72 human 440348 NPIPB15 nuclear pore complex intera... XM_011523498.3 33.6% 25.5% (many diffs)
73 human 440348 NPIPB15 nuclear pore complex intera... XM_017023248.1 33.6% 25.5% (many diffs)
74 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022823.2 32% 24.4% (many diffs)
75 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756354.2 28.4% (many diffs)
76 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030214.2 28% 25.2% (many diffs)
77 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022836.2 27.8% 24.6% (many diffs)
78 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022840.2 27.8% 24.6% (many diffs)
79 human 105369154 PKD1P6-NPIPP1 PKD1P6-NPIPP1 readthrough NR_123722.1 23.1% (many diffs)
80 human 728741 NPIPB6 nuclear pore complex intera... XM_005255743.3 17.9% 13.6% (many diffs)
81 human 440353 NPIPB12 nuclear pore complex intera... NM_001355401.1 17.7% 12.6% (many diffs)
82 human 440345 NPIPB4 nuclear pore complex intera... XM_024450289.1 17.6% 12.7% (many diffs)
83 human 728888 NPIPB11 nuclear pore complex intera... NM_001310137.2 14.7% 10.7% (many diffs)
84 human 100132247 NPIPB5 nuclear pore complex intera... NM_001135865.1 14.6% 10.4% (many diffs)
85 human 23117 NPIPB3 nuclear pore complex intera... NM_130464.3 14.6% 10.4% (many diffs)
86 human 613037 NPIPB13 nuclear pore complex intera... NM_001321892.1 14.5% 10.4% (many diffs)
87 human 440345 NPIPB4 nuclear pore complex intera... XM_017023247.1 14.5% 10.4% (many diffs)
88 human 440345 NPIPB4 nuclear pore complex intera... XM_024450285.1 14.5% 10.4% (many diffs)
89 human 728888 NPIPB11 nuclear pore complex intera... XM_024450420.1 14.3% 10.9% (many diffs)
90 human 440345 NPIPB4 nuclear pore complex intera... XM_024450284.1 13.6% 9.7% (many diffs)
91 human 440345 NPIPB4 nuclear pore complex intera... NM_001310148.1 13.5% 9.8% (many diffs)
92 human 440345 NPIPB4 nuclear pore complex intera... XM_017023244.2 13.5% 9.8% (many diffs)
93 human 440345 NPIPB4 nuclear pore complex intera... XM_024450283.1 13.2% 10% (many diffs)
94 human 105369154 PKD1P6-NPIPP1 PKD1P6-NPIPP1 readthrough NR_123721.1 12.8% (many diffs)
95 human 110006323 PKD1P4-NPIPA8 PKD1P4-NPIPA8 readthrough NR_146336.1 9.2% (many diffs)
96 human 109951030 PKD1P3-NPIPA1 PKD1P3-NPIPA1 readthrough NR_146231.1 9.1% (many diffs)
97 human 339044 PKD1P1 polycystin 1, transient rec... NR_036447.1 8.8% (many diffs)
98 human 110006322 PKD1P5-LOC105376752 PKD1P5-LOC105376752 readthr... NR_146331.1 5.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
765
ORF length:
696
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gttttgttgc ttaggatatg aatggctgag cggaggctgt aaaacctggc
121actctgcttg ggttatcaat actctggctg accatcgtca tcgtgggact gactttggtg
181gaagtccttg gttacttatc attactgtgt ttctgagaag ttataaattt gccatctccc
241tctgcacaag ttacctttgt gtgtctttcc tgaagactat cttcccgtct caaaatggac
301atgatggatc cacggatgta cagcagagag ccaggaggtc caactgccgt agacaggaag
361gaattaaaat tgtcctggaa gacatcttta ctttatggag acaggtggaa accaaagttc
421gagctaaaat ccgtaagatg aaggtgacaa caaaagtcaa ccgtcatgac aaaatcaatg
481gaaagaggaa gaccgccaaa gaacatctga ggaaactaag catgaaagaa cgtgagcacg
541gagaaaagga gaggcaggtg tcagaggcag aggaaaatgg gaaattggat atgaaagaaa
601tacacaccta catggaaatg tttcaacgtg cgcaagcgtt gcggcggcgg gcagaggact
661actacagatg caaaatcacc ccttctgcaa gaaagcctct ttgcaaccgg tgcacttaca
721atttggtatt acctgggagt gaaaagaaat attacagcca tgccttgcca actttcttgt
781acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
841gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.