Construct: ORF ccsbBroadEn_00214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001853.1_s300c1, BRDN0000395432
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN1 (823)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 823 | CAPN1 | calpain 1 | NM_001198868.2 | 100% | 100% | |
| 2 | human | 823 | CAPN1 | calpain 1 | NM_001198869.1 | 100% | 100% | |
| 3 | human | 823 | CAPN1 | calpain 1 | NM_005186.4 | 100% | 100% | |
| 4 | human | 823 | CAPN1 | calpain 1 | XM_006718698.2 | 100% | 100% | |
| 5 | human | 823 | CAPN1 | calpain 1 | XM_011545292.1 | 100% | 100% | |
| 6 | human | 823 | CAPN1 | calpain 1 | NR_040008.2 | 70.1% | 1_87del;353_354ins70;2160_2882del | |
| 7 | mouse | 12333 | Capn1 | calpain 1 | NM_001110504.1 | 85.8% | 89.2% | (many diffs) |
| 8 | mouse | 12333 | Capn1 | calpain 1 | NM_007600.3 | 85.8% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2208
- ORF length:
- 2142
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC GGAGGAGATC ATCACGCCGG TGTACTGCAC TGGGGTGTCA GCCCAAGTGC 121 AGAAGCAGCG GGCCAGGGAG CTGGGCCTGG GCCGCCATGA GAATGCCATC AAGTACCTGG 181 GCCAGGATTA TGAGCAGCTG CGGGTGCGAT GCCTGCAGAG TGGGACCCTC TTCCGTGATG 241 AGGCCTTCCC CCCGGTACCC CAGAGCCTGG GTTACAAGGA CCTGGGTCCC AATTCCTCCA 301 AGACCTATGG CATCAAGTGG AAGCGTCCCA CGGAACTGCT GTCAAACCCC CAGTTCATTG 361 TGGATGGAGC TACCCGCACA GACATCTGCC AGGGAGCACT GGGGGACTGC TGGCTCTTGG 421 CGGCCATCGC CTCCCTCACT CTCAACGACA CCCTCCTGCA CCGAGTGGTT CCGCACGGCC 481 AGAGCTTCCA GAATGGCTAT GCCGGCATCT TCCATTTCCA GCTGTGGCAA TTTGGGGAGT 541 GGGTGGACGT GGTCGTGGAT GACCTGCTGC CCATCAAGGA CGGGAAGCTA GTGTTCGTGC 601 ACTCTGCCGA AGGCAACGAG TTCTGGAGCG CCCTGCTTGA GAAGGCCTAT GCCAAGGTAA 661 ATGGCAGCTA CGAGGCCCTG TCAGGGGGCA GCACCTCAGA GGGCTTTGAG GACTTCACAG 721 GCGGGGTTAC CGAGTGGTAC GAGTTGCGCA AGGCTCCCAG TGACCTCTAC CAGATCATCC 781 TCAAGGCGCT GGAGCGGGGC TCCCTGCTGG GCTGCTCCAT AGACATCTCC AGCGTTCTAG 841 ACATGGAGGC CATCACTTTC AAGAAGTTGG TGAAGGGCCA TGCCTACTCT GTGACCGGGG 901 CCAAGCAGGT GAACTACCGA GGCCAGGTGG TGAGCCTGAT CCGGATGCGG AACCCCTGGG 961 GCGAGGTGGA GTGGACGGGA GCCTGGAGCG ACAGCTCCTC AGAGTGGAAC AACGTGGACC 1021 CATATGAACG GGACCAGCTC CGGGTCAAGA TGGAGGACGG GGAGTTCTGG ATGTCATTCC 1081 GAGACTTCAT GCGGGAGTTC ACCCGCCTGG AGATCTGCAA CCTCACACCC GACGCCCTCA 1141 AGAGCCGGAC CATCCGCAAA TGGAACACCA CACTCTACGA AGGCACCTGG CGGCGGGGGA 1201 GCACCGCGGG GGGCTGCCGA AACTACCCAG CCACCTTCTG GGTGAACCCT CAGTTCAAGA 1261 TCCGGCTGGA TGAGACGGAT GACCCGGACG ACTACGGGGA CCGCGAGTCA GGCTGCAGCT 1321 TCGTGCTCGC CCTTATGCAG AAGCACCGTC GCCGCGAGCG CCGCTTCGGC CGCGACATGG 1381 AGACTATTGG CTTCGCGGTC TACGAGGTCC CTCCGGAGCT GGTGGGCCAG CCGGCCGTAC 1441 ACTTGAAGCG TGACTTCTTC CTGGCCAATG CGTCTCGGGC GCGCTCAGAG CAGTTCATCA 1501 ACCTGCGAGA GGTCAGCACC CGCTTCCGCC TGCCACCCGG GGAGTATGTG GTGGTGCCCT 1561 CCACCTTCGA GCCCAACAAG GAGGGCGACT TCGTGCTGCG CTTCTTCTCA GAGAAGAGTG 1621 CTGGGACTGT GGAGCTGGAT GACCAGATCC AGGCCAATCT CCCCGATGAG CAAGTGCTCT 1681 CAGAAGAGGA GATTGACGAG AACTTCAAGG CCCTCTTCAG GCAGCTGGCA GGGGAGGACA 1741 TGGAGATCAG CGTGAAGGAG TTGCGGACAA TCCTCAATAG GATCATCAGC AAACACAAAG 1801 ACCTGCGGAC CAAGGGCTTC AGCCTAGAGT CGTGCCGCAG CATGGTGAAC CTCATGGATC 1861 GTGATGGCAA TGGGAAGCTG GGCCTGGTGG AGTTCAACAT CCTGTGGAAC CGCATCCGGA 1921 ATTACCTGTC CATCTTCCGG AAGTTTGACC TGGACAAGTC GGGCAGCATG AGTGCCTACG 1981 AGATGCGGAT GGCCATTGAG TCGGCAGGCT TCAAGCTCAA CAAGAAGCTG TACGAGCTCA 2041 TCATCACCCG CTACTCGGAG CCCGACCTGG CGGTCGACTT TGACAATTTC GTTTGCTGCC 2101 TGGTGCGGCT AGAGACCATG TTCCGATTTT TCAAAACTCT GGACACAGAT CTGGATGGAG 2161 TTGTGACCTT TGACTTGTTT AAGTGGTTGC AGCTGACCAT GTTTGCATGC CCAACTTTCT 2221 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac