Construct: ORF ccsbBroadEn_00443

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016618.1_s300c1, BRDN0000385568
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHFR (1719)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1719 DHFR dihydrofolate reductase NM_000791.4 100% 100%
2 human 200895 DHFR2 dihydrofolate reductase 2 NM_001195643.2 94.8% 91.9% (many diffs)
3 human 200895 DHFR2 dihydrofolate reductase 2 NM_176815.5 94.8% 91.9% (many diffs)
4 human 200895 DHFR2 dihydrofolate reductase 2 XM_011512537.3 94.8% 91.9% (many diffs)
5 human 1719 DHFR dihydrofolate reductase NM_001290354.2 72.1% 72.1% 0_1ins156
6 human 1719 DHFR dihydrofolate reductase NM_001290357.2 68.9% 64.7% 369_370ins116;387_388ins58
7 human 1720 DHFRP3 dihydrofolate reductase pse... NR_033423.1 53% (many diffs)
8 human 1719 DHFR dihydrofolate reductase NR_110936.2 9.7% (many diffs)
9 mouse 13361 Dhfr dihydrofolate reductase NM_010049.3 88.4% 89.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
627
ORF length:
561
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGT TGGTTCGCTA AACTGCATCG TCGCTGTGTC CCAGAACATG GGCATCGGCA
121AGAACGGGGA CCTGCCCTGG CCACCGCTCA GGAATGAATT CAGATATTTC CAGAGAATGA
181CCACAACCTC TTCAGTAGAA GGTAAACAGA ATCTGGTGAT TATGGGTAAG AAGACCTGGT
241TCTCCATTCC TGAGAAGAAT CGACCTTTAA AGGGTAGAAT TAATTTAGTT CTCAGCAGAG
301AACTCAAGGA ACCTCCACAA GGAGCTCATT TTCTTTCCAG AAGTCTAGAT GATGCCTTAA
361AACTTACTGA ACAACCAGAA TTAGCAAATA AAGTAGACAT GGTCTGGATA GTTGGTGGCA
421GTTCTGTTTA TAAGGAAGCC ATGAATCACC CAGGCCATCT TAAACTATTT GTGACAAGGA
481TCATGCAAGA CTTTGAAAGT GACACGTTTT TTCCAGAAAT TGATTTGGAG AAATATAAAC
541TTCTGCCAGA ATACCCAGGT GTTCTCTCTG ATGTCCAGGA GGAGAAAGGC ATTAAGTACA
601AATTTGAAGT ATATGAGAAG AATGATTACC CAACTTTCTT GTACAAAGTt ggcattataa
661gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)