Construct: ORF ccsbBroadEn_01703

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010205.1_s300c1, BRDN0000395637
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM3 (7170)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7170 TPM3 tropomyosin 3 NM_152263.4 100% 100%
2 human 7170 TPM3 tropomyosin 3 NM_001364682.1 95.7% 94.7% (many diffs)
3 human 7170 TPM3 tropomyosin 3 NM_001364681.2 93.2% 93.3% (many diffs)
4 human 7170 TPM3 tropomyosin 3 NM_001364679.2 92.6% 92.9% (many diffs)
5 human 7170 TPM3 tropomyosin 3 NM_001364680.2 88.5% 87.7% (many diffs)
6 human 7168 TPM1 tropomyosin 1 NM_001018005.2 80% 90.8% (many diffs)
7 human 7168 TPM1 tropomyosin 1 NM_001365779.1 79.9% 90.5% (many diffs)
8 human 7170 TPM3 tropomyosin 3 NM_001349679.2 79.7% 74.9% (many diffs)
9 human 7169 TPM2 tropomyosin 2 NM_003289.3 79.6% 85.9% (many diffs)
10 human 7169 TPM2 tropomyosin 2 XM_017015091.2 79.6% 85.9% (many diffs)
11 human 7170 TPM3 tropomyosin 3 NM_001278189.2 75.7% 69.7% (many diffs)
12 human 7170 TPM3 tropomyosin 3 NM_001364683.1 75.7% 69.7% (many diffs)
13 human 7170 TPM3 tropomyosin 3 NM_001043353.2 73.7% 68.3% (many diffs)
14 human 7170 TPM3 tropomyosin 3 NM_001043351.2 73.2% 68% (many diffs)
15 human 7170 TPM3 tropomyosin 3 NM_001043352.2 69.8% 63.2% (many diffs)
16 human 7170 TPM3 tropomyosin 3 NM_153649.4 69.3% 62.8% (many diffs)
17 human 7170 TPM3 tropomyosin 3 NM_001278190.1 62.9% 55.6% (many diffs)
18 human 7170 TPM3 tropomyosin 3 NM_001278188.2 51.1% 45.9% (many diffs)
19 human 7170 TPM3 tropomyosin 3 NM_001278191.2 49.5% 48.4% (many diffs)
20 human 7170 TPM3 tropomyosin 3 NR_103461.2 20.6% (many diffs)
21 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001293748.1 93.8% 98.9% (many diffs)
22 mouse 59069 Tpm3 tropomyosin 3, gamma NM_022314.3 82.7% 87% (many diffs)
23 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164248.1 80.5% 91.2% (many diffs)
24 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164251.1 78% 86.3% (many diffs)
25 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319678.1 76.5% 74.5% (many diffs)
26 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501838.1 76.4% 74.2% (many diffs)
27 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501836.2 75% 73% (many diffs)
28 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001271764.1 72.4% 69.4% (many diffs)
29 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319677.1 72.4% 69.4% (many diffs)
30 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501835.2 70.9% 68% (many diffs)
31 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001293749.1 70.6% 68% (many diffs)
32 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001253740.1 70.3% 68% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
921
ORF length:
855
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAT GGAGGCCATC AAGAAAAAGA TGCAGATGCT GAAGTTAGAC AAGGAGAATG
121CTCTGGATCG GGCAGAGCAA GCTGAAGCTG AGCAGAAGCA GGCAGAAGAA AGAAGTAAAC
181AGCTGGAGGA TGAGCTGGCA GCCATGCAGA AGAAGCTGAA AGGGACAGAG GATGAGCTGG
241ACAAGTATTC TGAAGCTTTG AAGGATGCCC AGGAGAAGCT GGAACTGGCA GAGAAGAAGG
301CTGCTGATGC TGAGGCTGAG GTGGCCTCCT TGAACCGTAG GATCCAGCTG GTTGAAGAAG
361AGCTGGACCG TGCTCAGGAG CGCCTGGCCA CTGCCCTGCA AAAGCTGGAA GAAGCTGAAA
421AAGCTGCTGA TGAGAGTGAG AGAGGTATGA AGGTTATTGA AAACCGGGCC TTAAAAGATG
481AAGAAAAGAT GGAACTCCAG GAAATCCAAC TCAAAGAAGC TAAGCACATT GCAGAAGAGG
541CAGATAGGAA GTATGAAGAG GTGGCTCGTA AGTTGGTGAT CATTGAAGGA GACTTGGAAC
601GCACAGAGGA ACGAGCTGAG CTGGCAGAGT CTAAGTGTTC TGAGCTGGAG GAGGAGCTGA
661AGAATGTCAC CAACAACCTC AAGTCTCTTG AGGCTCAGGC GGAGAAGTAC TCTCAAAAAG
721AAGATAAATA TGAGGAAGAA ATCAAGATTC TTACTGATAA ACTCAAGGAG GCAGAGACCC
781GTGCTGAGTT TGCTGAGAGA TCGGTAGCCA AGCTGGAAAA GACAATTGAT GACCTGGAAG
841ATGAGCTCTA TGCCCAGAAA CTGAAGTACA AGGCCATTAG CGAGGAGCTG GACCACGCCC
901TCAATGACAT GACCTCTATA TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
961attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)