Transcript: Human NM_001364682.1

Homo sapiens tropomyosin 3 (TPM3), transcript variant 16, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
TPM3 (7170)
Length:
7064
CDS:
83..940

Additional Resources:

NCBI RefSeq record:
NM_001364682.1
NBCI Gene record:
TPM3 (7170)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001364682.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274851 CTCAAGCTGAACAAGATTAAA pLKO_005 1366 3UTR 100% 15.000 12.000 N TPM3 n/a
2 TRCN0000029526 AGAGGTATGAAGGTTATTGAA pLKO.1 458 CDS 100% 5.625 3.938 N TPM3 n/a
3 TRCN0000029524 CCTCAATGACATGACCTCTAT pLKO.1 916 CDS 100% 4.950 3.465 N TPM3 n/a
4 TRCN0000274848 TCAAGATTCTTACTGATAAAC pLKO_005 759 CDS 100% 13.200 7.920 N TPM3 n/a
5 TRCN0000029528 ACATTGCAGAAGAGGCAGATA pLKO.1 543 CDS 100% 4.950 2.970 N TPM3 n/a
6 TRCN0000274850 ACATTGCAGAAGAGGCAGATA pLKO_005 543 CDS 100% 4.950 2.970 N TPM3 n/a
7 TRCN0000029527 GCTGAGCAGAAGCAGGCAGAA pLKO.1 164 CDS 100% 1.350 0.810 N TPM3 n/a
8 TRCN0000139826 CCTCCTGAATAGCTGGGATTA pLKO.1 6614 3UTR 100% 10.800 5.400 Y SYNPO2 n/a
9 TRCN0000274911 CTCGTAAGTTGGTGATCATTG pLKO_005 582 CDS 100% 10.800 5.400 Y TPM3 n/a
10 TRCN0000274912 TCCAGGAAATCCAACTCAAAG pLKO_005 513 CDS 100% 10.800 5.400 Y TPM3 n/a
11 TRCN0000084008 CGCCTATAATCCCAGCACTTT pLKO.1 1914 3UTR 100% 4.950 2.475 Y NPHS1 n/a
12 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4964 3UTR 100% 4.950 2.475 Y ERAP2 n/a
13 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 3291 3UTR 100% 4.950 2.475 Y LOC387873 n/a
14 TRCN0000162786 CAGAACCTGAAGTGTCTGAGT pLKO.1 689 CDS 100% 2.640 1.320 Y TPM3P9 n/a
15 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 3732 3UTR 100% 1.080 0.540 Y GPR83 n/a
16 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 3732 3UTR 100% 1.080 0.540 Y MYORG n/a
17 TRCN0000425801 CGTTGCCGAGAGATGGATGAA pLKO_005 650 CDS 100% 4.950 2.970 N Tpm3 n/a
18 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4965 3UTR 100% 13.200 6.600 Y LIAS n/a
19 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 5019 3UTR 100% 4.050 2.025 Y INTS7 n/a
20 TRCN0000168807 GCTGATCTCAAACTCCTGATA pLKO.1 6702 3UTR 100% 4.950 2.475 Y TMEM105 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001364682.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01703 pDONR223 100% 95.7% 94.7% None (many diffs) n/a
2 ccsbBroad304_01703 pLX_304 0% 95.7% 94.7% V5 (many diffs) n/a
3 TRCN0000466488 ACAATCCGGTTACCCTTTTAGTAG pLX_317 33.2% 95.7% 94.7% V5 (many diffs) n/a
4 TRCN0000474312 TCGGGCATGCTATAACTGGACCTG pLX_317 60.2% 73.8% 68% V5 (not translated due to frame shift) (many diffs) n/a
5 ccsbBroadEn_15614 pDONR223 0% 73.2% 68% None (many diffs) n/a
6 ccsbBroad304_15614 pLX_304 0% 73.2% 68% V5 (many diffs) n/a
7 ccsbBroadEn_11198 pDONR223 100% 51.5% 50.1% None (many diffs) n/a
8 ccsbBroad304_11198 pLX_304 0% 51.5% 50.1% V5 (many diffs) n/a
9 TRCN0000473724 CAACCCTTACTTATGAATACCCCC pLX_317 86% 51.5% 50.1% V5 (many diffs) n/a
10 ccsbBroadEn_13244 pDONR223 100% 25.7% 23.5% None (many diffs) n/a
11 ccsbBroad304_13244 pLX_304 0% 25.7% 23.5% V5 (many diffs) n/a
12 TRCN0000467014 TTGCCATGATGAACCCCCTTACCA pLX_317 100% 25.7% 23.5% V5 (many diffs) n/a
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