Transcript: Human NM_001043351.2

Homo sapiens tropomyosin 3 (TPM3), transcript variant Tpm3.2, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
TPM3 (7170)
Length:
3177
CDS:
100..846

Additional Resources:

NCBI RefSeq record:
NM_001043351.2
NBCI Gene record:
TPM3 (7170)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001043351.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029526 AGAGGTATGAAGGTTATTGAA pLKO.1 364 CDS 100% 5.625 3.938 N TPM3 n/a
2 TRCN0000029525 GCTGAAGAATGTCACCAACAA pLKO.1 579 CDS 100% 4.950 3.465 N TPM3 n/a
3 TRCN0000274848 TCAAGATTCTTACTGATAAAC pLKO_005 665 CDS 100% 13.200 7.920 N TPM3 n/a
4 TRCN0000029528 ACATTGCAGAAGAGGCAGATA pLKO.1 449 CDS 100% 4.950 2.970 N TPM3 n/a
5 TRCN0000274850 ACATTGCAGAAGAGGCAGATA pLKO_005 449 CDS 100% 4.950 2.970 N TPM3 n/a
6 TRCN0000274911 CTCGTAAGTTGGTGATCATTG pLKO_005 488 CDS 100% 10.800 5.400 Y TPM3 n/a
7 TRCN0000274912 TCCAGGAAATCCAACTCAAAG pLKO_005 419 CDS 100% 10.800 5.400 Y TPM3 n/a
8 TRCN0000159731 GATAAACTGAAATGCACCAAA pLKO.1 763 CDS 100% 4.950 2.475 Y TPM3P9 n/a
9 TRCN0000161977 GCTTGACCTGAATGAGATGTA pLKO.1 825 CDS 100% 4.950 2.475 Y TPM3P9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001043351.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15614 pDONR223 0% 95% 93.9% None (many diffs) n/a
2 ccsbBroad304_15614 pLX_304 0% 95% 93.9% V5 (many diffs) n/a
3 TRCN0000474312 TCGGGCATGCTATAACTGGACCTG pLX_317 60.2% 94.9% 92.7% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_01703 pDONR223 100% 73.2% 68% None (many diffs) n/a
5 ccsbBroad304_01703 pLX_304 0% 73.2% 68% V5 (many diffs) n/a
6 TRCN0000466488 ACAATCCGGTTACCCTTTTAGTAG pLX_317 33.2% 73.2% 68% V5 (many diffs) n/a
7 ccsbBroadEn_11198 pDONR223 100% 56.6% 55.6% None (many diffs) n/a
8 ccsbBroad304_11198 pLX_304 0% 56.6% 55.6% V5 (many diffs) n/a
9 TRCN0000473724 CAACCCTTACTTATGAATACCCCC pLX_317 86% 56.6% 55.6% V5 (many diffs) n/a
10 ccsbBroadEn_13244 pDONR223 100% 33.5% 29.8% None (many diffs) n/a
11 ccsbBroad304_13244 pLX_304 0% 33.5% 29.8% V5 (many diffs) n/a
12 TRCN0000467014 TTGCCATGATGAACCCCCTTACCA pLX_317 100% 33.5% 29.8% V5 (many diffs) n/a
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