Transcript: Human NM_001364679.2

Homo sapiens tropomyosin 3 (TPM3), transcript variant 13, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
TPM3 (7170)
Length:
3271
CDS:
83..940

Additional Resources:

NCBI RefSeq record:
NM_001364679.2
NBCI Gene record:
TPM3 (7170)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001364679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029526 AGAGGTATGAAGGTTATTGAA pLKO.1 458 CDS 100% 5.625 3.938 N TPM3 n/a
2 TRCN0000029525 GCTGAAGAATGTCACCAACAA pLKO.1 673 CDS 100% 4.950 3.465 N TPM3 n/a
3 TRCN0000274848 TCAAGATTCTTACTGATAAAC pLKO_005 759 CDS 100% 13.200 7.920 N TPM3 n/a
4 TRCN0000029528 ACATTGCAGAAGAGGCAGATA pLKO.1 543 CDS 100% 4.950 2.970 N TPM3 n/a
5 TRCN0000274850 ACATTGCAGAAGAGGCAGATA pLKO_005 543 CDS 100% 4.950 2.970 N TPM3 n/a
6 TRCN0000029527 GCTGAGCAGAAGCAGGCAGAA pLKO.1 164 CDS 100% 1.350 0.810 N TPM3 n/a
7 TRCN0000274911 CTCGTAAGTTGGTGATCATTG pLKO_005 582 CDS 100% 10.800 5.400 Y TPM3 n/a
8 TRCN0000274912 TCCAGGAAATCCAACTCAAAG pLKO_005 513 CDS 100% 10.800 5.400 Y TPM3 n/a
9 TRCN0000159731 GATAAACTGAAATGCACCAAA pLKO.1 857 CDS 100% 4.950 2.475 Y TPM3P9 n/a
10 TRCN0000161977 GCTTGACCTGAATGAGATGTA pLKO.1 919 CDS 100% 4.950 2.475 Y TPM3P9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001364679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01703 pDONR223 100% 92.6% 92.9% None (many diffs) n/a
2 ccsbBroad304_01703 pLX_304 0% 92.6% 92.9% V5 (many diffs) n/a
3 TRCN0000466488 ACAATCCGGTTACCCTTTTAGTAG pLX_317 33.2% 92.6% 92.9% V5 (many diffs) n/a
4 ccsbBroadEn_15614 pDONR223 0% 75.7% 69.7% None (many diffs) n/a
5 ccsbBroad304_15614 pLX_304 0% 75.7% 69.7% V5 (many diffs) n/a
6 TRCN0000474312 TCGGGCATGCTATAACTGGACCTG pLX_317 60.2% 75.6% 68.7% V5 (not translated due to frame shift) (many diffs) n/a
7 ccsbBroadEn_11198 pDONR223 100% 49.5% 48.4% None (many diffs) n/a
8 ccsbBroad304_11198 pLX_304 0% 49.5% 48.4% V5 (many diffs) n/a
9 TRCN0000473724 CAACCCTTACTTATGAATACCCCC pLX_317 86% 49.5% 48.4% V5 (many diffs) n/a
10 ccsbBroadEn_13244 pDONR223 100% 29.1% 25.9% None (many diffs) n/a
11 ccsbBroad304_13244 pLX_304 0% 29.1% 25.9% V5 (many diffs) n/a
12 TRCN0000467014 TTGCCATGATGAACCCCCTTACCA pLX_317 100% 29.1% 25.9% V5 (many diffs) n/a
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