Transcript: Human NM_152263.4

Homo sapiens tropomyosin 3 (TPM3), transcript variant Tpm3.12, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
TPM3 (7170)
Length:
7064
CDS:
83..940

Additional Resources:

NCBI RefSeq record:
NM_152263.4
NBCI Gene record:
TPM3 (7170)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_152263.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274851 CTCAAGCTGAACAAGATTAAA pLKO_005 1366 3UTR 100% 15.000 12.000 N TPM3 n/a
2 TRCN0000029526 AGAGGTATGAAGGTTATTGAA pLKO.1 458 CDS 100% 5.625 3.938 N TPM3 n/a
3 TRCN0000029524 CCTCAATGACATGACCTCTAT pLKO.1 916 CDS 100% 4.950 3.465 N TPM3 n/a
4 TRCN0000029525 GCTGAAGAATGTCACCAACAA pLKO.1 673 CDS 100% 4.950 3.465 N TPM3 n/a
5 TRCN0000274848 TCAAGATTCTTACTGATAAAC pLKO_005 759 CDS 100% 13.200 7.920 N TPM3 n/a
6 TRCN0000029528 ACATTGCAGAAGAGGCAGATA pLKO.1 543 CDS 100% 4.950 2.970 N TPM3 n/a
7 TRCN0000274850 ACATTGCAGAAGAGGCAGATA pLKO_005 543 CDS 100% 4.950 2.970 N TPM3 n/a
8 TRCN0000029527 GCTGAGCAGAAGCAGGCAGAA pLKO.1 164 CDS 100% 1.350 0.810 N TPM3 n/a
9 TRCN0000139826 CCTCCTGAATAGCTGGGATTA pLKO.1 6614 3UTR 100% 10.800 5.400 Y SYNPO2 n/a
10 TRCN0000274911 CTCGTAAGTTGGTGATCATTG pLKO_005 582 CDS 100% 10.800 5.400 Y TPM3 n/a
11 TRCN0000274912 TCCAGGAAATCCAACTCAAAG pLKO_005 513 CDS 100% 10.800 5.400 Y TPM3 n/a
12 TRCN0000084008 CGCCTATAATCCCAGCACTTT pLKO.1 1914 3UTR 100% 4.950 2.475 Y NPHS1 n/a
13 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4964 3UTR 100% 4.950 2.475 Y ERAP2 n/a
14 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 3291 3UTR 100% 4.950 2.475 Y LOC387873 n/a
15 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 3732 3UTR 100% 1.080 0.540 Y GPR83 n/a
16 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 3732 3UTR 100% 1.080 0.540 Y MYORG n/a
17 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4965 3UTR 100% 13.200 6.600 Y LIAS n/a
18 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 5019 3UTR 100% 4.050 2.025 Y INTS7 n/a
19 TRCN0000168807 GCTGATCTCAAACTCCTGATA pLKO.1 6702 3UTR 100% 4.950 2.475 Y TMEM105 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_152263.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01703 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01703 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466488 ACAATCCGGTTACCCTTTTAGTAG pLX_317 33.2% 100% 100% V5 n/a
4 TRCN0000474312 TCGGGCATGCTATAACTGGACCTG pLX_317 60.2% 69.9% 62.8% V5 (not translated due to frame shift) (many diffs) n/a
5 ccsbBroadEn_15614 pDONR223 0% 69.3% 62.8% None (many diffs) n/a
6 ccsbBroad304_15614 pLX_304 0% 69.3% 62.8% V5 (many diffs) n/a
7 ccsbBroadEn_11198 pDONR223 100% 55.4% 55.4% None 1_381del n/a
8 ccsbBroad304_11198 pLX_304 0% 55.4% 55.4% V5 1_381del n/a
9 TRCN0000473724 CAACCCTTACTTATGAATACCCCC pLX_317 86% 55.4% 55.4% V5 1_381del n/a
Download CSV

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