Construct: ORF ccsbBroadEn_02387

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003414.1_s300c1, BRDN0000392006
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPEG (10290)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10290 SPEG striated muscle enriched pr... NM_001173476.2 100% 100%
2 human 10290 SPEG striated muscle enriched pr... XM_024452526.1 100% 100%
3 human 10290 SPEG striated muscle enriched pr... XM_017003162.2 66.4% 66.4% 1_171del
4 human 10290 SPEG striated muscle enriched pr... XM_017003161.2 62.5% 62.2% (many diffs)
5 human 10290 SPEG striated muscle enriched pr... XR_001738587.2 7% (many diffs)
6 human 10290 SPEG striated muscle enriched pr... XM_017003159.2 6.1% 6% (many diffs)
7 human 10290 SPEG striated muscle enriched pr... XM_005246240.2 4.6% 4.5% 335_342delCACACCCT;344_345delAAinsCG;348_7254del
8 human 10290 SPEG striated muscle enriched pr... XM_005246241.1 4.6% 4.5% 335_342delCACACCCT;344_345delAAinsCG;348_7254del
9 human 10290 SPEG striated muscle enriched pr... XM_006712193.3 4.6% 4.5% 335_342delCACACCCT;344_345delAAinsCG;348_7254del
10 human 10290 SPEG striated muscle enriched pr... XM_017003158.2 4.6% 4.5% 335_342delCACACCCT;344_345delAAinsCG;348_7254del
11 human 10290 SPEG striated muscle enriched pr... XM_005246239.2 4.5% 4.4% (many diffs)
12 human 10290 SPEG striated muscle enriched pr... XM_005246242.4 4.5% 4.4% (many diffs)
13 human 10290 SPEG striated muscle enriched pr... XM_006712189.3 3.5% 3.5% (many diffs)
14 human 10290 SPEG striated muscle enriched pr... XM_005246237.2 3.5% 3.4% (many diffs)
15 human 10290 SPEG striated muscle enriched pr... XM_017003157.1 3.5% 3.4% (many diffs)
16 human 10290 SPEG striated muscle enriched pr... XM_011510483.2 3.5% 3.4% (many diffs)
17 human 10290 SPEG striated muscle enriched pr... NM_005876.5 3.4% 3.3% (many diffs)
18 human 10290 SPEG striated muscle enriched pr... XM_011510479.2 3.4% 3.3% (many diffs)
19 mouse 11790 Speg SPEG complex locus NM_001173477.1 87.6% 94.6% (many diffs)
20 mouse 11790 Speg SPEG complex locus XM_017312276.1 11.6% 12.5% (many diffs)
21 mouse 11790 Speg SPEG complex locus NM_001085371.1 11.4% 12.4% (many diffs)
22 mouse 11790 Speg SPEG complex locus XM_011238651.2 10.1% 10.9% (many diffs)
23 mouse 11790 Speg SPEG complex locus XM_011238650.2 5.6% 6% (many diffs)
24 mouse 11790 Speg SPEG complex locus XM_006496403.3 4% 4.3% (many diffs)
25 mouse 11790 Speg SPEG complex locus XM_006496404.3 4% 4.3% (many diffs)
26 mouse 11790 Speg SPEG complex locus XM_006496402.2 3.9% 4.2% (many diffs)
27 mouse 11790 Speg SPEG complex locus NM_001085370.1 3.8% 4.1% (many diffs)
28 mouse 11790 Speg SPEG complex locus XM_006496400.2 3.8% 4.1% (many diffs)
29 mouse 11790 Speg SPEG complex locus XM_006496401.2 3.8% 4.1% (many diffs)
30 mouse 11790 Speg SPEG complex locus XM_011238648.2 3.8% 4.1% (many diffs)
31 mouse 11790 Speg SPEG complex locus XM_011238649.2 3.8% 4.1% (many diffs)
32 mouse 11790 Speg SPEG complex locus XM_011238647.1 3.1% 3.3% (many diffs)
33 mouse 11790 Speg SPEG complex locus XM_017322375.1 3.1% 3.3% (many diffs)
34 mouse 11790 Speg SPEG complex locus XM_011238646.1 3.1% 3.3% (many diffs)
35 mouse 11790 Speg SPEG complex locus XM_011238645.1 3.1% 3.3% (many diffs)
36 mouse 11790 Speg SPEG complex locus XM_017322354.1 3% 3.2% (many diffs)
37 mouse 11790 Speg SPEG complex locus XM_011238644.1 3% 3.2% (many diffs)
38 mouse 11790 Speg SPEG complex locus NM_007463.4 3% 3.2% (many diffs)
39 mouse 11790 Speg SPEG complex locus XM_011238643.1 3% 3.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
405
ORF length:
339
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GCCCAGTCCC AGCCAGAACC GCCGTTCTTC TGACACTGGC TCCAAGGCAC
121CCCCCACCTT CAAGGTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA GATGTCATCA
181TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA AACCGCCAGC
241CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG TGCCGGCTGC
301GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG GTCAATGAGT
361ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGG CGAGTGCCCA ACTTTCTTGT
421ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)