Transcript: Human XM_006712193.3

PREDICTED: Homo sapiens striated muscle enriched protein kinase (SPEG), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPEG (10290)
Length:
11511
CDS:
3412..10668

Additional Resources:

NCBI RefSeq record:
XM_006712193.3
NBCI Gene record:
SPEG (10290)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145284 ACGTACACTGTGTCATGCGC pXPR_003 CGG 1157 16% 8 1.342 SPEG SPEG 77623
2 BRDN0001145668 GGGGAGGATGAGGACCATCG pXPR_003 AGG 2228 31% 16 0.0441 SPEG SPEG 77624
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006712193.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419399 CTATGCGGTCAGTGCTGTTAA pLKO_005 4263 CDS 100% 13.200 18.480 N SPEG n/a
2 TRCN0000429025 GTGTCTACAAGAGCGTCATTG pLKO_005 4631 CDS 100% 10.800 15.120 N SPEG n/a
3 TRCN0000199850 CCGGACATCATGTGGTACAAG pLKO.1 5404 CDS 100% 4.950 6.930 N SPEG n/a
4 TRCN0000037431 CTACACTTGCAAAGCGGTCAA pLKO.1 3681 CDS 100% 4.050 5.670 N SPEG n/a
5 TRCN0000037432 GCGTGGCGATGCTGGTTTCTA pLKO.1 3663 CDS 100% 1.875 2.625 N SPEG n/a
6 TRCN0000435415 GGGTGTGCTCACTTACATTAT pLKO_005 10305 CDS 100% 13.200 9.240 N SPEG n/a
7 TRCN0000419013 TGTTGTTACCTGGACTCATTT pLKO_005 4143 CDS 100% 13.200 9.240 N SPEG n/a
8 TRCN0000418263 ACAAACCAGACATCGTGTATG pLKO_005 5045 CDS 100% 10.800 7.560 N SPEG n/a
9 TRCN0000434726 ACTCTTCTGAGAAGGTCTTTG pLKO_005 9146 CDS 100% 10.800 7.560 N SPEG n/a
10 TRCN0000415476 AGTTTGTAGCACCCGAGATTG pLKO_005 6149 CDS 100% 10.800 7.560 N SPEG n/a
11 TRCN0000438295 CGCACATCTCCTGGATGAAAG pLKO_005 8702 CDS 100% 10.800 7.560 N SPEG n/a
12 TRCN0000438525 ACCGCAGAAGAGACCCTAGAA pLKO_005 6394 CDS 100% 4.950 3.465 N SPEG n/a
13 TRCN0000037429 CCACCTTCAAGGTCTCACTTA pLKO.1 3470 CDS 100% 4.950 3.465 N SPEG n/a
14 TRCN0000037430 CCAAGATGTCATCATGAGCAT pLKO.1 3513 CDS 100% 2.640 1.848 N SPEG n/a
15 TRCN0000037433 TCACTTATGGACCAGTCAGTA pLKO.1 3484 CDS 100% 4.950 2.970 N SPEG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006712193.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02387 pDONR223 100% 4.6% 4.5% None 335_342delCACACCCT;344_345delAAinsCG;348_7254del n/a
2 ccsbBroad304_02387 pLX_304 0% 4.6% 4.5% V5 335_342delCACACCCT;344_345delAAinsCG;348_7254del n/a
3 TRCN0000467901 AATAATGACTCCTACGGATTTTGC pLX_317 77.9% 4.6% 4.5% V5 335_342delCACACCCT;344_345delAAinsCG;348_7254del n/a
4 TRCN0000488546 TAATCTTAGTTCATACATGCTTTC pLX_317 58.7% 4.5% 4.4% V5 (many diffs) n/a
5 TRCN0000488863 GAAACGTACAGGTATTCTTTTTCG pLX_317 59.5% 4.5% 4.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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