Construct: ORF ccsbBroadEn_05497

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016890.1_s300c1, BRDN0000456314
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACTBL2 (345651)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 345651 ACTBL2 actin beta like 2 NM_001017992.4 100% 100%
2 human 60 ACTB actin beta NM_001101.5 81.6% 91.2% (many diffs)
3 human 71 ACTG1 actin gamma 1 NM_001199954.2 80.9% 90.9% (many diffs)
4 human 71 ACTG1 actin gamma 1 NM_001614.5 80.9% 90.9% (many diffs)
5 human 72 ACTG2 actin gamma 2, smooth muscle NM_001615.4 80.4% 87.7% (many diffs)
6 human 72 ACTG2 actin gamma 2, smooth muscle NM_001199893.2 71.4% 77.3% (many diffs)
7 mouse 238880 Actbl2 actin, beta-like 2 NM_175497.3 89.4% 97% (many diffs)
8 mouse 11461 Actb actin, beta NM_007393.5 81.4% 91.2% (many diffs)
9 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_009609.3 80.7% 90.9% (many diffs)
10 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 79.2% 87% (many diffs)
11 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 79.2% 87% (many diffs)
12 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_001313923.1 54.5% 62.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1197
ORF length:
1128
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GACTGACAAT GAGCTGTCTG CCTTGGTAGT GGATAATGGG TCAGGGATGT
121GCAAGGCAGG CTTTGGTGGT GACGATGCCC CCCGGGCTGT GTTCCCCTCC ATGATAGGGC
181GTCCTCGACA CCAGGGCGTT ATGGTAGGCA TGGGCCAGAA GGACTGCTAC GTGGGAGATG
241AGGCTCAGAG CAAGAGAGGC GTCCTGACCC TGAAGTATCC TATCGAGCAT GGAGTGGTCA
301CCAACTGGGA CGATATGGAA AAGATCTGGT ACCACACATT CTACAATGAG CTCCGTGTGG
361CACCAGATGA GCATCCCATC CTCCTCACCG AGGCACCCCT GAACCCCAAG ATCAACCGGG
421AAAAGATGAC TCAGATCATG TTCGAGGCTT TCAACACACC AGCCATGTAT GTCGCCATCC
481AGGCTGTGCT GTCCCTCTAT GCCTCAGGAC GGACCACAGG CATCGTGATG GATTCTGGGG
541ATGGGGTCAC TCACATCGTG CCCATCTATG AAGGTTATGC CCTGCCTCAT GCCATTCTAC
601GCCTGGATCT GGCAGGGAGA GACCTGACTG ATTACCTCAT GAAGATCCTG ACAGAGCGAG
661GCTATAACTT CACCACCACT GCTGAGCGGG AGATTGTGCG AGATGTCAAA GAGAAGCTGT
721GCTACGTAGC CCTGGACTTT GAGCAGGAGA TGGTCAGGGC AGCCGCATCC TCCTCACCGG
781AACGGAGCTA TGAACTTCCT GATGGGCAGG TGATCACCAT TGGGAATGAA CGCTTCCGAT
841GCCCTGAAGC CATTTTCCAG CCTTCCTTTC TGGGCATTGA GTCCAGTGGG ATCCATGAGA
901CAACCTTCAA CTCTATCATG AAGTGCGATG TGGATATTCG CAAGGATCTC TATGCCAACA
961CCGTGTTATC TGGAGGGAGC ACCATGTACC CAGGCATTGC TGACAGGATG CAGAAAGAAA
1021TCATAACCCT GGCACCCAGC ACCATGAAAA TCAAGATCAT AGCTCCCCCA GAGCGGAAGT
1081ATTCTGTTTG GATTGGGGGC TCCATCCTTG CCAGCCTGTC CACATTCCAG CAGATGTGGA
1141TCAGTAAGCA GGAATATGAT GAGGCTGGTC CTCCTATCGT TCACAGAAAA TGTTTCTTGC
1201CAACTTTCTT GTACAAAGTT GGcattataa gaaagcattg cttatcaatt tgttgcaacg
1261aac

Download FASTA (ORF) (Full)