Transcript: Mouse NM_007393.5

Mus musculus actin, beta (Actb), mRNA.

Source:
NCBI, updated 2017-06-15
Taxon:
Mus musculus (mouse)
Gene:
Actb (11461)
Length:
1935
CDS:
110..1237

Additional Resources:

NCBI RefSeq record:
NM_007393.5
NBCI Gene record:
Actb (11461)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007393.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305266 GGCTGAGGACTTTGTACATTG pLKO_005 1494 3UTR 100% 10.800 7.560 N Actb n/a
2 TRCN0000090899 CCATTGAACATGGCATTGTTA pLKO.1 318 CDS 100% 5.625 3.375 N Actb n/a
3 TRCN0000090898 GCATTGCTTCTGTGTAAATTA pLKO.1 1679 3UTR 100% 15.000 7.500 Y Actb n/a
4 TRCN0000309146 GCATTGCTTCTGTGTAAATTA pLKO_005 1679 3UTR 100% 15.000 7.500 Y Actb n/a
5 TRCN0000375594 ACGGCCAGGTCATCACTATTG pLKO_005 840 CDS 100% 10.800 5.400 Y Actb n/a
6 TRCN0000089841 CCTGACAGACTACCTCATGAA pLKO.1 661 CDS 100% 4.950 2.475 Y Actg-ps1 n/a
7 TRCN0000090901 CGTGCGTGACATCAAAGAGAA pLKO.1 733 CDS 100% 4.950 2.475 Y Actb n/a
8 TRCN0000309123 CGTGCGTGACATCAAAGAGAA pLKO_005 733 CDS 100% 4.950 2.475 Y Actb n/a
9 TRCN0000090900 CCTTCCTTCTTGGGTATGGAA pLKO.1 899 CDS 100% 3.000 1.500 Y Actb n/a
10 TRCN0000315605 CCTTCCTTCTTGGGTATGGAA pLKO_005 899 CDS 100% 3.000 1.500 Y Actb n/a
11 TRCN0000090902 CCAGATCATGTTTGAGACCTT pLKO.1 469 CDS 100% 2.640 1.320 Y Actb n/a
12 TRCN0000090377 CACACCTTCTACAATGAGCTT pLKO.1 371 CDS 100% 2.640 1.320 Y Gm5388 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007393.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05763 pDONR223 100% 91.8% 99.7% None (many diffs) n/a
2 ccsbBroad304_05763 pLX_304 53.1% 91.8% 99.7% V5 (many diffs) n/a
3 TRCN0000470279 TCAAGCCTGGAACGGGCTCACGTC pLX_317 31.7% 91.8% 99.7% V5 (many diffs) n/a
4 ccsbBroadEn_05764 pDONR223 100% 89.4% 98.9% None (many diffs) n/a
5 ccsbBroad304_05764 pLX_304 0% 89.4% 98.9% V5 (many diffs) n/a
6 TRCN0000466781 AATTGTGGTGCTTGTTGCCGCCTG pLX_317 31.7% 89.4% 98.9% V5 (many diffs) n/a
7 ccsbBroadEn_15351 pDONR223 0% 89.3% 98.9% None (many diffs) n/a
8 ccsbBroad304_15351 pLX_304 0% 89.3% 98.9% V5 (many diffs) n/a
9 ccsbBroadEn_13808 pDONR223 100% 89.3% 98.4% None (many diffs) n/a
10 ccsbBroad304_13808 pLX_304 0% 89.3% 98.4% V5 (not translated due to frame shift) (many diffs) n/a
11 TRCN0000471742 AATGGGAGTTCTCACGTCAGGTCC pLX_317 44.8% 89.3% 98.4% V5 (not translated due to frame shift) (many diffs) n/a
12 ccsbBroadEn_00015 pDONR223 100% 84.8% 93.3% None (many diffs) n/a
13 ccsbBroad304_00015 pLX_304 0% 84.8% 93.3% V5 (many diffs) n/a
14 TRCN0000468012 TCTATATTCTTCCCTCACTCATAA pLX_317 37.9% 84.8% 93.3% V5 (many diffs) n/a
15 ccsbBroadEn_00014 pDONR223 100% 82.4% 93.3% None (many diffs) n/a
16 ccsbBroad304_00014 pLX_304 0% 82.4% 93.3% V5 (not translated due to prior stop codon) (many diffs) n/a
17 TRCN0000467679 GCTACTGCTTTGAGGGTTATAACT pLX_317 37.9% 82.4% 93.3% V5 (not translated due to prior stop codon) (many diffs) n/a
18 ccsbBroadEn_05497 pDONR223 100% 81.4% 91.2% None (many diffs) n/a
19 ccsbBroad304_05497 pLX_304 0% 81.4% 91.2% V5 (many diffs) n/a
20 TRCN0000480996 TCGCGGAGACTCACCCAATCCCGC pLX_317 35.4% 81.4% 91.2% V5 (many diffs) n/a
21 ccsbBroadEn_14514 pDONR223 100% 49.4% 45% None (many diffs) n/a
22 ccsbBroad304_14514 pLX_304 0% 49.4% 45% V5 (not translated due to frame shift) (many diffs) n/a
23 TRCN0000481436 GCTCGTTTACTAGTATTTTTAATC pLX_317 64.2% 49.4% 45% V5 (not translated due to frame shift) (many diffs) n/a
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