Construct: ORF ccsbBroadEn_05709

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014058.1_s300c1, BRDN0000391230
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACOT1 (641371)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 641371 ACOT1 acyl-CoA thioesterase 1 NM_001037161.2 100% 100%
2 human 10965 ACOT2 acyl-CoA thioesterase 2 NM_006821.5 86.2% 86.1% (many diffs)
3 human 641371 ACOT1 acyl-CoA thioesterase 1 XM_017021590.1 67.7% 65.6% (many diffs)
4 human 641371 ACOT1 acyl-CoA thioesterase 1 XM_017021589.1 67.5% 63.9% (many diffs)
5 human 10965 ACOT2 acyl-CoA thioesterase 2 NM_001364177.1 66.5% 64.6% (many diffs)
6 human 641371 ACOT1 acyl-CoA thioesterase 1 XM_017021591.1 61.5% 61.5% 0_1ins486
7 human 641371 ACOT1 acyl-CoA thioesterase 1 XM_017021592.1 61.5% 61.5% 0_1ins486
8 human 10965 ACOT2 acyl-CoA thioesterase 2 NR_046028.1 60% (many diffs)
9 human 10965 ACOT2 acyl-CoA thioesterase 2 NM_001364178.1 48.2% 45.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1332
ORF length:
1263
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCGGCGACG CTGATCCTGG AGCCCGCGGG CCGCTGCTGC TGGGACGAAC
121CGGTGCGAAT CGCCGTGCGC GGCCTAGCCC CGGAGCAGCC GGTCACGCTG CGCGCGTCCC
181TGCGCGACGA GAAGGGCGCG CTTTTCCAGG CCCACGCGCG CTACCGCGCC GACACCCTTG
241GCGAGCTGGA CCTGGAGCGC GCGCCCGCGC TGGGCGGCAG CTTCGCGGGG CTTGAGCCCA
301TGGGGCTGCT CTGGGCCTTG GAGCCCGAGA AACCCTTGGT GCGGCTGGTG AAGCGCGACG
361TGCGAACGCC CTTGGCCGTG GAGCTGGAGG TGCTGGATGG CCACGACCCC GACCCCGGGC
421GGCTGCTGTG CCGGGTGCGG CACGAGCGCT ACTTCCTCCC GCCCGGGGTG CGGCGCGAGC
481CGGTGCGCGC GGGCCGGGTG CGAGGCACGC TCTTCCTGCC GCCAGAACCT GGGCCCTTTC
541CTGGCATTGT GGACATGTTC GGAACTGGAG GTGGCCTGCT GGAGTATCGG GCTAGTCTGC
601TGGCTGGGAA GGGTTTTGCT GTGATGGCTC TGGCTTACTA TAACTATGAA GACCTCCCCA
661AGACCATGGA GACGCTCCAT CTGGAGTACT TTGAAGAAGC TGTGAACTAC TTGCTCAGTC
721ATCCTGAGGT AAAAGGTCCA GGAGTTGGGC TGCTTGGAAT TTCCAAAGGG GGTGAGCTCT
781GCCTTTCCAT GGCCTCTTTC CTGAAGGGCA TCACGGCTGC TGTCGTCATC AACGGCTCTG
841TGGCCAATGT TGGGGGAACC TTACGCTACA AGGGCGAGAC CCTGCCCCCT GTGGGCGTCA
901ACAGAAATCG CATCAAGGTG ACCAAAGATG GCTATGCAGA CATTGTGGAT GTCCTGAACA
961GCCCTTTGGA AGGACCTGAC CAGAAGAGCT TCATTCCTGT GGAAAGGGCA GAGAGCACCT
1021TCCTGTTCCT GGTAGGTCAG GATGACCACA ACTGGAAGAG TGAGTTCTAT GCTAATGAGG
1081CCTGTAAACG CTTGCAGGCC CATGGGAGGA GAAAGCCCCA GATCATCTGT TACCCAGAGA
1141CAGGGCACTA TATTGAGCCT CCTTACTTCC CCCTGTGTCG GGCTTCCCTG CATGCCTTGG
1201TGGGCAGTCC TATTATCTGG GGAGGGGAGC CCAGGGCTCA TGCCATGGCT CAGGTGGATG
1261CTTGGAAACA ACTCCAGACT TTCTTCCACA AACACTTGGG TGGCCACGAG GGGACAATCC
1321CATCAAAAGT GTTGCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
1381caatttgttg caacgaac

Download FASTA (ORF) (Full)