Construct: ORF ccsbBroadEn_06002

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004739.1_s300c1, BRDN0000385046
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRNA7 (1139)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021884.1 99.8% 100% 726C>T
2 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... NM_148911.1 99.5% 100% (many diffs)
3 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_005254750.3 99.5% 100% (many diffs)
4 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521177.2 79.9% 80% 1_240del;966C>T
5 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... NM_139320.1 77.5% 77.9% (many diffs)
6 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_011522153.2 77.5% 77.9% (many diffs)
7 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021883.2 72.8% 72.9% 1_357del;1083C>T
8 human 1139 CHRNA7 cholinergic receptor nicoti... XM_017021882.1 72.7% 72.7% 1_360del;1086C>T
9 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521176.3 65.9% 66% 1_495del;1221C>T
10 human 1139 CHRNA7 cholinergic receptor nicoti... NM_000746.6 63.8% 63.9% 1_543del;1269C>T
11 human 1139 CHRNA7 cholinergic receptor nicoti... NM_001190455.3 60.3% 60.4% 1_630del;1356C>T
12 human 89832 CHRFAM7A CHRNA7 (exons 5-10) and FAM... XM_011522155.3 48.1% 33.7% (many diffs)
13 human 1139 CHRNA7 cholinergic receptor nicoti... XM_011521178.3 39.6% 27.7% 1_543del;879_880ins110;1140_1141ins256
14 human 1139 CHRNA7 cholinergic receptor nicoti... NR_046324.1 30.3% 1_465del;1191C>T;1429_3166del
15 mouse 11441 Chrna7 cholinergic receptor, nicot... NM_007390.3 55.1% 59.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1029
ORF length:
963
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA GGAGGCAGAT ATCAGTGGCT ATATCCCCAA TGGAGAATGG GACCTAGTGG
121GAATCCCCGG CAAGAGGAGT GAAAGGTTCT ATGAGTGCTG CAAAGAGCCC TACCCCGATG
181TCACCTTCAC AGTGACCATG CGCCGCAGGA CGCTCTACTA TGGCCTCAAC CTGCTGATCC
241CCTGTGTGCT CATCTCCGCC CTCGCCCTGC TGGTGTTCCT GCTTCCTGCA GATTCCGGGG
301AGAAGATTTC CCTGGGGATA ACAGTCTTAC TCTCTCTTAC CGTCTTCATG CTGCTCGTGG
361CTGAGATCAT GCCCGCAACA TCCGATTCGG TACCATTGAT AGCCCAGTAC TTCGCCAGCA
421CCATGATCAT CGTGGGCCTC TCGGTGGTGG TGACAGTGAT CGTGCTGCAG TACCACCACC
481ACGACCCCGA CGGGGGCAAG ATGCCCAAGT GGACCAGAGT CATCCTTCTG AACTGGTGCG
541CGTGGTTCCT GCGAATGAAG AGGCCCGGGG AGGACAAGGT GCGCCCGGCC TGCCAGCACA
601AGCAGCGGCG CTGCAGCCTG GCCAGTGTGG AGATGAGCGC CGTGGCGCCG CCGCCCGCCA
661GCAACGGGAA CCTGCTGTAC ATCGGCTTCC GCGGCCTGGA CGGCGTGCAC TGTGTCCCGA
721CCCCCGACTC TGGGGTAGTG TGTGGCCGCA TGGCCTGCTC CCCCACGCAC GATGAGCACC
781TCCTGCACGG TGGGCAACCC CCCGAGGGGG ACCCGGACTT GGCCAAGATC CTGGAGGAGG
841TCCGCTACAT TGCCAACCGC TTCCGCTGCC AGGACGAAAG CGAGGCGGTC TGCAGCGAGT
901GGAAGTTCGC CGCCTGTGTG GTGGACCGCC TGTGCCTCAT GGCCTTCTCG GTCTTCACCA
961TCATCTGCAC CATCGGCATC CTGATGTCGG CTCCCAACTT CGTGGAGGCC GTGTCCAAAG
1021ACTTTGCGTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
1081tttgttgcaa cgaac

Download FASTA (ORF) (Full)