Transcript: Human NM_001190455.3

Homo sapiens cholinergic receptor nicotinic alpha 7 subunit (CHRNA7), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
CHRNA7 (1139)
Length:
6236
CDS:
81..1676

Additional Resources:

NCBI RefSeq record:
NM_001190455.3
NBCI Gene record:
CHRNA7 (1139)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001190455.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437248 ACAGTGCTGATGAGCGCTTTG pLKO_005 514 CDS 100% 6.000 8.400 N CHRNA7 n/a
2 TRCN0000061170 CGAGTTCCAGAGGAAGCTTTA pLKO.1 236 CDS 100% 10.800 8.640 N CHRNA7 n/a
3 TRCN0000061169 GATCACTATTTACAGTGGAAT pLKO.1 417 CDS 100% 4.950 3.960 N CHRNA7 n/a
4 TRCN0000438526 GGGTGAAGACTGTTCGTTTCC pLKO_005 454 CDS 100% 4.050 3.240 N CHRNA7 n/a
5 TRCN0000102957 CAGATTTGGAAACCAGACATT pLKO.1 483 CDS 100% 4.950 3.465 N Chrna7 n/a
6 TRCN0000061171 GAAACCAGACATTCTTCTCTA pLKO.1 491 CDS 100% 4.950 3.465 N CHRNA7 n/a
7 TRCN0000434356 GCAAATGTCTTGGACAGATCA pLKO_005 401 CDS 100% 4.950 3.465 N CHRNA7 n/a
8 TRCN0000061172 GCAACCACTCACCGTCTACTT pLKO.1 311 CDS 100% 4.950 3.465 N CHRNA7 n/a
9 TRCN0000436990 TGCAGATCATGGACGTGGATG pLKO_005 346 CDS 100% 4.050 2.835 N CHRNA7 n/a
10 TRCN0000438008 GACAGTGATCGTGCTGCAGTA pLKO_005 1097 CDS 100% 4.050 2.430 N CHRNA7 n/a
11 TRCN0000430981 GCCACCATGCCTGGCTAATTT pLKO_005 3118 3UTR 100% 15.000 7.500 Y GTF2IRD2 n/a
12 TRCN0000241869 GGCAGATATCAGTGGCTATAT pLKO_005 719 CDS 100% 13.200 6.600 Y CHRFAM7A n/a
13 TRCN0000241868 AGAGGAGTGAAAGGTTCTATG pLKO_005 778 CDS 100% 10.800 5.400 Y CHRFAM7A n/a
14 TRCN0000060471 GCAGCACTGCAAACTGAAGTT pLKO.1 650 CDS 100% 4.950 2.475 Y CHRFAM7A n/a
15 TRCN0000060469 CGTGTCCAAAGACTTTGCGTA pLKO.1 1655 CDS 100% 2.640 1.320 Y CHRFAM7A n/a
16 TRCN0000241871 TACCTGCCTCCAGGCATATTC pLKO_005 585 CDS 100% 0.000 0.000 Y CHRFAM7A n/a
17 TRCN0000157610 GTGGCATGATCTCAGCTCATT pLKO.1 3022 3UTR 100% 4.950 2.475 Y CCNJL n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190455.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488120 GCAAGGCAGAAGTCCCTCTATTTA pLX_317 17.1% 94.4% 94.5% V5 (not translated due to prior stop codon) 55_141del;777G>A n/a
2 ccsbBroadEn_09274 pDONR223 100% 75.7% 73.8% None (many diffs) n/a
3 ccsbBroad304_09274 pLX_304 0% 75.7% 73.8% V5 (many diffs) n/a
4 TRCN0000470647 TTCCCAGCTACGACTCCCCGATTC pLX_317 38.3% 75.7% 73.8% V5 (many diffs) n/a
5 TRCN0000487766 CATCTTTACATCGCTATTCACAGA pLX_317 20.7% 75.7% 73.8% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000488341 CTACCCCCGCGTCGCTGCAATTCC pLX_317 24.4% 65.5% 63.6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_06002 pDONR223 100% 60.3% 60.4% None 1_630del;1356C>T n/a
8 ccsbBroad304_06002 pLX_304 0% 60.3% 60.4% V5 1_630del;1356C>T n/a
9 TRCN0000470538 CAGTACCTTGGTTTGCTAGCCTAT pLX_317 51.9% 60.3% 60.4% V5 1_630del;1356C>T n/a
10 ccsbBroadEn_09275 pDONR223 100% 60.2% 60.4% None (many diffs) n/a
11 ccsbBroad304_09275 pLX_304 0% 60.2% 60.4% V5 (many diffs) n/a
12 TRCN0000477932 ATAGCAGGATTCACGGGAACGAAT pLX_317 25.9% 60.2% 60.4% V5 (many diffs) n/a
Download CSV