Construct: ORF ccsbBroadEn_06149

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000256.1_s300c1, BRDN0000397951
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF4B (1975)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1975 EIF4B eukaryotic translation init... NM_001417.7 99.8% 100% 1644C>A;1755C>T
2 human 1975 EIF4B eukaryotic translation init... NM_001300821.3 99% 99% 1307_1321del;1659C>A;1770C>T
3 human 1975 EIF4B eukaryotic translation init... NM_001330654.2 93.5% 93.6% 360_361ins117;1527C>A;1638C>T
4 mouse 75705 Eif4b eukaryotic translation init... NM_145625.3 90% 94.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1899
ORF length:
1833
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCCTCAGCA AAAAAGAAGA ATAAGAAGGG GAAGACTATC TCCCTAACAG
121ACTTTCTGGC TGAGGATGGG GGTACTGGTG GAGGAAGCAC CTATGTTTCC AAACCAGTCA
181GCTGGGCTGA TGAAACGGAT GACCTGGAAG GAGATGTTTC GACCACTTGG CACAGTAACG
241ATGACGATGT GTATAGGGCG CCTCCAATTG ACCGTTCCAT CCTTCCCACT GCTCCACGGG
301CTGCTCGGGA ACCCAATATC GACCGGAGCC GTCTTCCCAA ATCGCCACCC TACACTGCTT
361TTCTAGGAAA CCTACCCTAT GATGTTACAG AAGAGTCAAT TAAGGAATTC TTTCGAGGAT
421TAAATATCAG TGCAGTGCGT TTACCACGTG AACCCAGCAA TCCAGAGAGG TTGAAAGGTT
481TTGGTTATGC TGAATTTGAG GACCTGGATT CCCTGCTCAG TGCCCTGAGT CTCAATGAAG
541AGTCTCTAGG TAACAGGAGA ATTCGAGTGG ACGTTGCTGA TCAAGCACAG GATAAAGACA
601GGGATGATCG TTCTTTTGGC CGTGATAGAA ATCGGGATTC TGACAAAACA GATACAGACT
661GGAGGGCTCG TCCTGCTACA GACAGCTTTG ATGACTACCC ACCTAGAAGA GGTGATGATA
721GCTTTGGAGA CAAGTATCGA GATCGTTATG ATTCAGACCG GTATCGGGAT GGGTATCGGG
781ATGGGTATCG GGATGGCCCA CGCCGGGATA TGGATCGATA TGGTGGCCGG GATCGCTATG
841ATGACCGAGG CAGCAGAGAC TATGATAGAG GCTATGATTC CCGGATAGGC AGTGGCAGAA
901GAGCATTTGG CAGTGGGTAT CGCAGGGATG ATGACTACAG AGGAGGCGGG GACCGCTATG
961AAGACCGATA TGACAGACGG GATGATCGGT CGTGGAGCTC CAGAGATGAT TACTCTCGGG
1021ATGATTATAG GCGTGATGAT AGAGGTCCCC CCCAAAGACC CAAACTGAAT CTAAAGCCTC
1081GGAGTACTCC TAAGGAAGAT GATTCCTCTG CTAGTACCTC CCAGTCCACT CGAGCTGCTT
1141CTATCTTTGG AGGGGCAAAG CCTGTTGACA CAGCTGCTAG AGAAAGAGAA GTAGAAGAAC
1201GGCTACAGAA GGAACAAGAG AAGTTGCAGC GTCAGCTGGA TGAGCCAAAA CTAGAACGAC
1261GGCCTCGGGA GAGACACCCA AGCTGGCGAA GTGAAGAAAC TCAGGAACGG GAACGGTCGA
1321GGACAGGAAG TGAGTCATCA CAAACTGGGA CCTCCACCAC ATCTAGCAGA AATGCACGAA
1381GGAGAGAGAG TGAGAAGTCT CTAGAAAATG AAACACTCAA TAAGGAGGAA GATTGCCACT
1441CTCCAACTTC TAAACCTCCC AAACCTGATC AGCCCCTAAA GGTAATGCCA GCCCCTCCAC
1501CAAAGGAGAA TGCTTGGGTG AAGCGAAGTT CTAACCCTCC TGCTCGATCT CAGAGCTCAG
1561ACACAGAGCA GCAGTCCCCT ACAAGTGGTG GGGGAAAAGT AGCTCCAGCT CAACCATCTG
1621AGGAAGGACC AGGAAGGAAA GATGAAAATA AAGTAGATGG GATGAATGCC CCAAAAGGCC
1681AAACTGGGAA CTCTAGCCGT GGTCCAGGAG ACGGAGGGAA CAGAGACCAC TGGAAGGAGT
1741CAGATAGGAA AGATGGCAAA AAGGATCAAG ACTCCAGATC TGCACCTGAG CCAAAGAAAC
1801CTGAGGAAAA TCCAGCTTCT AAGTTCAGTT CTGCAAGCAA GTATGCTGCT CTCTCTGTTG
1861ATGGTGAAGA TGAAAATGAG GGAGAAGATT ATGCCGAATA CCCAACTTTC TTGTACAAAG
1921Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)