Transcript: Human NM_001417.7

Homo sapiens eukaryotic translation initiation factor 4B (EIF4B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
EIF4B (1975)
Length:
3856
CDS:
29..1864

Additional Resources:

NCBI RefSeq record:
NM_001417.7
NBCI Gene record:
EIF4B (1975)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001417.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236426 GACAAGTATCGAGATCGTTAT pLKO_005 692 CDS 100% 10.800 15.120 N EIF4B n/a
2 TRCN0000236425 GCCGGGATATGGATCGATATG pLKO_005 765 CDS 100% 10.800 15.120 N EIF4B n/a
3 TRCN0000236427 TGGTAATGACAGTGATATAAT pLKO_005 2178 3UTR 100% 15.000 10.500 N EIF4B n/a
4 TRCN0000236424 TGTTACAGAAGAGTCAATTAA pLKO_005 346 CDS 100% 15.000 10.500 N EIF4B n/a
5 TRCN0000236423 GACCGCTATGAAGACCGATAT pLKO_005 914 CDS 100% 10.800 7.560 N EIF4B n/a
6 TRCN0000062602 CTACCCTATGATGTTACAGAA pLKO.1 335 CDS 100% 4.950 3.465 N EIF4B n/a
7 TRCN0000062598 GCGGAGAAACACCTTGATCTT pLKO.1 3292 3UTR 100% 4.950 3.465 N EIF4B n/a
8 TRCN0000062600 CCAACTTCTAAACCTCCCAAA pLKO.1 1406 CDS 100% 4.050 2.835 N EIF4B n/a
9 TRCN0000062601 CGGGATGATTATAGGCGTGAT pLKO.1 980 CDS 100% 4.050 2.835 N EIF4B n/a
10 TRCN0000062599 GCCGTGATAGAAATCGGGATT pLKO.1 582 CDS 100% 4.050 2.835 N EIF4B n/a
11 TRCN0000427090 ACCGGTATCGGGATGGGTATA pLKO_005 720 CDS 100% 10.800 15.120 N Eif4b n/a
12 TRCN0000416638 GACAAGTATCGAGATCGTTAC pLKO_005 692 CDS 100% 6.000 8.400 N Eif4b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001417.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06149 pDONR223 100% 99.8% 100% None 1644C>A;1755C>T n/a
2 ccsbBroad304_06149 pLX_304 0% 99.8% 100% V5 1644C>A;1755C>T n/a
3 TRCN0000465580 TAACTTGACGAAAGGACCCTGTGC pLX_317 15.7% 99.8% 100% V5 1644C>A;1755C>T n/a
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