Construct: ORF ccsbBroadEn_06254

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002235.1_s300c1, BRDN0000390333
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2597 GAPDH glyceraldehyde-3-phosphate ... NM_001289745.3 99.8% 100% 381T>C;885T>C
2 human 2597 GAPDH glyceraldehyde-3-phosphate ... NM_001289746.2 99.8% 100% 381T>C;885T>C
3 human 2597 GAPDH glyceraldehyde-3-phosphate ... NM_002046.7 99.8% 100% 381T>C;885T>C
4 human 2597 GAPDH glyceraldehyde-3-phosphate ... NM_001357943.2 94.4% 94.6% 128_129ins54;327T>C;831T>C
5 human 2597 GAPDH glyceraldehyde-3-phosphate ... NM_001256799.3 87.2% 87.4% 0_1ins126;255T>C;759T>C
6 human 2597 GAPDH glyceraldehyde-3-phosphate ... NR_152150.2 54.4% (many diffs)
7 mouse 14433 Gapdh glyceraldehyde-3-phosphate ... NM_008084.3 88.9% 93.4% (many diffs)
8 mouse 100042025 Gapdh-ps15 glyceraldehyde-3-phosphate ... XM_001476707.5 88.6% 93.4% (many diffs)
9 mouse 14433 Gapdh glyceraldehyde-3-phosphate ... NM_001289726.1 82.6% 87.4% (many diffs)
10 mouse 14433 Gapdh glyceraldehyde-3-phosphate ... XM_017321385.1 78.4% 77.8% (many diffs)
11 mouse 433273 Gm5523 predicted pseudogene 5523; ... NR_004447.1 68.1% (many diffs)
12 mouse 378954 3000002C10Rik RIKEN cDNA 3000002C10 gene;... NR_033215.1 67.5% (many diffs)
13 mouse 100151693 Gm10364 predicted pseudogene 10364;... NR_073535.1 61.8% (many diffs)
14 mouse 629557 Gm6981 predicted pseudogene 6981; ... NR_023357.1 60% (many diffs)
15 mouse 654472 Gm12070 predicted gene 12070; glyce... NR_002890.1 51.1% (many diffs)
16 mouse 277333 Gm5069 predicted pseudogene 5069; ... NR_003623.1 24.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1071
ORF length:
1005
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG GAAGGTGAAG GTCGGAGTCA ACGGATTTGG TCGTATTGGG CGCCTGGTCA
121CCAGGGCTGC TTTTAACTCT GGTAAAGTGG ATATTGTTGC CATCAATGAC CCCTTCATTG
181ACCTCAACTA CATGGTTTAC ATGTTCCAAT ATGATTCCAC CCATGGCAAA TTCCATGGCA
241CCGTCAAGGC TGAGAACGGG AAGCTTGTCA TCAATGGAAA TCCCATCACC ATCTTCCAGG
301AGCGAGATCC CTCCAAAATC AAGTGGGGCG ATGCTGGCGC TGAGTACGTC GTGGAGTCCA
361CTGGCGTCTT CACCACCATG GAGAAGGCTG GGGCTCATTT GCAGGGGGGA GCCAAAAGGG
421TCATCATCTC TGCCCCCTCT GCTGACGCCC CCATGTTCGT CATGGGTGTG AACCATGAGA
481AGTATGACAA CAGCCTCAAG ATCATCAGCA ATGCCTCCTG CACCACCAAC TGCTTAGCAC
541CCCTGGCCAA GGTCATCCAT GACAACTTTG GTATCGTGGA AGGACTCATG ACCACAGTCC
601ATGCCATCAC TGCCACCCAG AAGACTGTGG ATGGCCCCTC CGGGAAACTG TGGCGTGATG
661GCCGCGGGGC TCTCCAGAAC ATCATCCCTG CCTCTACTGG CGCTGCCAAG GCTGTGGGCA
721AGGTCATCCC TGAGCTGAAC GGGAAGCTCA CTGGCATGGC CTTCCGTGTC CCCACTGCCA
781ACGTGTCAGT GGTGGACCTG ACCTGCCGTC TAGAAAAACC TGCCAAATAT GATGACATCA
841AGAAGGTGGT GAAGCAGGCG TCGGAGGGCC CCCTCAAGGG CATCCTGGGC TACACTGAGC
901ACCAGGTGGT CTCCTCTGAC TTCAACAGCG ACACCCACTC CTCCACCTTC GACGCTGGGG
961CTGGCATTGC CCTCAACGAC CACTTTGTCA AGCTCATTTC CTGGTATGAC AACGAATTTG
1021GCTACAGCAA CAGGGTGGTG GACCTCATGG CCCACATGGC CTCCAAGGAG TACCCAACTT
1081TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)