Construct: ORF ccsbBroadEn_06559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000884.1_s300c1, BRDN0000386959
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2 (4133)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4133 | MAP2 | microtubule associated prot... | NM_001039538.1 | 99.9% | 100% | 21T>C |
| 2 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004138.2 | 99.9% | 100% | 21T>C |
| 3 | human | 4133 | MAP2 | microtubule associated prot... | NM_031847.2 | 89.7% | 89.8% | 21T>C;516_517ins171 |
| 4 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452910.1 | 89.7% | 89.8% | 21T>C;516_517ins171 |
| 5 | human | 4133 | MAP2 | microtubule associated prot... | NM_001363913.1 | 84.1% | 84.2% | 21T>C;516_517ins171;1005_1006ins93 |
| 6 | human | 4133 | MAP2 | microtubule associated prot... | NM_031845.2 | 84.1% | 84.2% | 21T>C;516_517ins171;1005_1006ins93 |
| 7 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452911.1 | 84.1% | 84.2% | 21T>C;516_517ins171;1005_1006ins93 |
| 8 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004116.2 | 29.8% | 27.9% | (many diffs) |
| 9 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452897.1 | 29.8% | 27.8% | (many diffs) |
| 10 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452899.1 | 29.8% | 27.8% | (many diffs) |
| 11 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004114.2 | 29.7% | 27.7% | (many diffs) |
| 12 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452896.1 | 29.7% | 27.7% | (many diffs) |
| 13 | human | 4133 | MAP2 | microtubule associated prot... | XM_005246565.1 | 29.5% | 25.3% | (many diffs) |
| 14 | human | 4133 | MAP2 | microtubule associated prot... | XM_005246566.1 | 29.5% | 25.3% | (many diffs) |
| 15 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004130.1 | 29.5% | 25.3% | (many diffs) |
| 16 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004131.1 | 28.8% | 22.1% | (many diffs) |
| 17 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004132.1 | 28.8% | 22.1% | (many diffs) |
| 18 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004125.2 | 28.2% | 26.2% | (many diffs) |
| 19 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004126.2 | 28.2% | 26.2% | (many diffs) |
| 20 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452905.1 | 28.2% | 26.2% | (many diffs) |
| 21 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452906.1 | 28.2% | 26.2% | (many diffs) |
| 22 | human | 4133 | MAP2 | microtubule associated prot... | NM_001363910.1 | 28.1% | 26.2% | (many diffs) |
| 23 | human | 4133 | MAP2 | microtubule associated prot... | NM_001363911.1 | 28.1% | 26.2% | (many diffs) |
| 24 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004124.2 | 28.1% | 26.2% | (many diffs) |
| 25 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004129.2 | 28.1% | 26.1% | (many diffs) |
| 26 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004128.2 | 28.1% | 26.1% | (many diffs) |
| 27 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004119.2 | 28.1% | 26.1% | (many diffs) |
| 28 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004121.2 | 28.1% | 26.1% | (many diffs) |
| 29 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452901.1 | 28.1% | 26.1% | (many diffs) |
| 30 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004127.2 | 28.1% | 26.1% | (many diffs) |
| 31 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452907.1 | 28.1% | 26.1% | (many diffs) |
| 32 | human | 4133 | MAP2 | microtubule associated prot... | NM_002374.3 | 28.1% | 26.1% | (many diffs) |
| 33 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004118.2 | 28.1% | 26.1% | (many diffs) |
| 34 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452900.1 | 28.1% | 26.1% | (many diffs) |
| 35 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004120.2 | 28% | 26% | (many diffs) |
| 36 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004122.2 | 28% | 26% | (many diffs) |
| 37 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452902.1 | 28% | 26% | (many diffs) |
| 38 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004137.1 | 27.1% | 20.3% | (many diffs) |
| 39 | human | 4133 | MAP2 | microtubule associated prot... | XM_011511197.3 | 27.1% | 29.1% | (many diffs) |
| 40 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004113.2 | 27.1% | 29.1% | (many diffs) |
| 41 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452895.1 | 27.1% | 29.1% | (many diffs) |
| 42 | human | 4133 | MAP2 | microtubule associated prot... | XM_011511196.3 | 27.1% | 29.2% | (many diffs) |
| 43 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452894.1 | 27.1% | 29.2% | (many diffs) |
| 44 | human | 4133 | MAP2 | microtubule associated prot... | XM_011511195.2 | 27.1% | 29.1% | (many diffs) |
| 45 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004112.2 | 27% | 29.1% | (many diffs) |
| 46 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452891.1 | 27% | 29.1% | (many diffs) |
| 47 | human | 4133 | MAP2 | microtubule associated prot... | XM_024452893.1 | 27% | 29.1% | (many diffs) |
| 48 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004136.1 | 27% | 20.3% | (many diffs) |
| 49 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004133.1 | 27% | 20.3% | (many diffs) |
| 50 | human | 4133 | MAP2 | microtubule associated prot... | XM_017004134.1 | 27% | 20.3% | (many diffs) |
| 51 | mouse | 17756 | Map2 | microtubule-associated prot... | NM_001039934.1 | 79.3% | 84.6% | (many diffs) |
| 52 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495759.4 | 79.3% | 84.6% | (many diffs) |
| 53 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495760.2 | 79.3% | 84.6% | (many diffs) |
| 54 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251982.1 | 79.3% | 84.6% | (many diffs) |
| 55 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251986.1 | 79.3% | 84.6% | (many diffs) |
| 56 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495761.4 | 79.1% | 84.4% | (many diffs) |
| 57 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251989.1 | 79.1% | 84.4% | (many diffs) |
| 58 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251990.1 | 79.1% | 84.4% | (many diffs) |
| 59 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251993.1 | 79.1% | 84.4% | (many diffs) |
| 60 | mouse | 17756 | Map2 | microtubule-associated prot... | NM_008632.2 | 73.9% | 79% | (many diffs) |
| 61 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495762.4 | 73.9% | 79% | (many diffs) |
| 62 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_017319194.2 | 73.9% | 79% | (many diffs) |
| 63 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251996.1 | 73.9% | 79% | (many diffs) |
| 64 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495753.3 | 24.2% | 26.7% | (many diffs) |
| 65 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_030251971.1 | 24.2% | 26.7% | (many diffs) |
| 66 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495751.3 | 24.1% | 26.6% | (many diffs) |
| 67 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_017319165.2 | 24.1% | 26.6% | (many diffs) |
| 68 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495746.3 | 24.1% | 26.8% | (many diffs) |
| 69 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495747.4 | 24.1% | 26.8% | (many diffs) |
| 70 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495748.4 | 24.1% | 26.8% | (many diffs) |
| 71 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495749.4 | 24.1% | 26.8% | (many diffs) |
| 72 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_011238448.2 | 24.1% | 26.8% | (many diffs) |
| 73 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495752.3 | 24% | 26.7% | (many diffs) |
| 74 | mouse | 17756 | Map2 | microtubule-associated prot... | XM_006495750.3 | 24% | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1743
- ORF length:
- 1677
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AGATGAACGG AAAGACGAAG CAAAGGCACC TCACTGGACC TCAGCACCGC 121 TAACAGAGGC ATCTGCACAC TCACATCCAC CTGAGATTAA GGATCAAGGC GGAGCAGGGG 181 AAGGACTTGT CCGAAGCGCC AATGGATTCC CATACAGGGA GGATGAAGAG GGTGCCTTTG 241 GAGAGCATGG GTCACAGGGC ACCTATTCAA ATACCAAAGA GAATGGGATC AACGGAGAGC 301 TGACCTCAGC TGACAGAGAA ACAGCAGAGG AGGTGTCTGC AAGGATAGTT CAAGTAGTCA 361 CTGCTGAGGC TGTAGCAGTC CTGAAAGGTG AACAAGAGAA AGAAGCTCAA CATAAAGACC 421 AGACTGCAGC TCTGCCTTTA GCAGCTGAAG AAACAGCTAA TCTGCCTCCT TCTCCACCCC 481 CATCACCTGC CTCAGAACAG ACTGTCACAG TGGAGGAAGC AGCAGGTGGG GAATCAGCTC 541 TGGCTCCCAG TGTATTTAAA CAGGCAAAGG ACAAAGTCTC TAATTCTACC TTGTCAAAGA 601 TTCCTGCTTT ACAGGGTAGC ACAAAGTCCC CAAGATACAG CTCAGCCTGC CCTAGCACGA 661 CTAAAAGGGC TACATTTTCT GACAGTTTAT TAATACAGCC CACCTCAGCA GGCTCCACAG 721 ACCGTTTGCC ATACTCAAAA TCAGGGAACA AGGACGGAGT AACCAAGAGC CCAGAAAAGC 781 GCTCTTCTCT CCCAAGACCT TCCTCCATTC TCCCTCCTCG GCGAGGTGTG TCAGGAGACA 841 GAGATGAGAA TTCCTTCTCT CTCAACAGTT CTATCTCTTC TTCAGCACGG CGGACCACCA 901 GGTCAGAGCC AATTCGCAGA GCAGGGAAGA GTGGTACCTC AACACCCACT ACCCCTGGGT 961 CTACTGCCAT CACTCCTGGC ACCCCACCAA GTTATTCTTC ACGCACACCA GGCACTCCTG 1021 GAACCCCTAG CTATCCCAGG ACCCCTCACA CACCAGGAAC CCCCAAGTCT GCCATCTTGG 1081 TGCCGAGTGA GAAGAAGGTC GCCATCATAC GTACTCCTCC AAAATCTCCT GCGACTCCCA 1141 AGCAGCTTCG GCTTATTAAC CAACCACTGC CAGACCTGAA GAATGTCAAA TCCAAAATCG 1201 GATCAACAGA CAACATCAAA TACCAGCCTA AAGGGGGGCA GGTTAGGATT TTAAACAAGA 1261 AGATCGATTT TAGCAAAGTT CAGTCCAGAT GTGGTTCCAA GGATAACATC AAACATTCGG 1321 CTGGGGGCGG AAATGTACAA ATTGTTACCA AGAAAATAGA CCTAAGCCAT GTGACATCCA 1381 AATGTGGCTC TCTGAAGAAC ATCCGCCACA GGCCAGGTGG CGGACGTGTG AAAATTGAGA 1441 GTGTAAAACT AGATTTCAAA GAAAAGGCCC AAGCTAAAGT TGGTTCTCTT GATAATGCTC 1501 ATCATGTACC TGGAGGTGGT AATGTCAAGA TTGACAGCCA AAAGTTGAAC TTCAGAGAGC 1561 ATGCTAAAGC CCGTGTGGAC CATGGGGCTG AGATCATTAC ACAGTCCCCA GGCAGATCCA 1621 GCGTGGCATC ACCCCGACGA CTCAGCAATG TCTCCTCGTC TGGAAGCATC AACCTGCTCG 1681 AATCTCCTCA GCTTGCCACT TTGGCTGAGG ATGTCACTGC TGCACTCGCT AAGCAGGGCT 1741 TGTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt 1801 gcaacgaac