Construct: ORF ccsbBroadEn_06786

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013259.1_s300c1, BRDN0000394852
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MASP1 (5648)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5648 MASP1 mannan binding lectin serin... NM_139125.3 99.9% 100% 1374T>C;1851G>A
2 human 5648 MASP1 mannan binding lectin serin... XM_011512989.2 98.9% 98.9% (many diffs)
3 human 5648 MASP1 mannan binding lectin serin... XM_017006869.1 96.3% 96.4% 0_1ins78;1296T>C;1773G>A
4 human 5648 MASP1 mannan binding lectin serin... XM_017006872.1 84.3% 84.4% 0_1ins339;1035T>C;1512G>A
5 human 5648 MASP1 mannan binding lectin serin... NM_001879.5 77.8% 72.1% (many diffs)
6 human 5648 MASP1 mannan binding lectin serin... XM_011512990.2 77.1% 71.3% (many diffs)
7 human 5648 MASP1 mannan binding lectin serin... XM_017006871.1 75.7% 68.6% (many diffs)
8 human 5648 MASP1 mannan binding lectin serin... XM_017006870.2 71% 61.1% (many diffs)
9 human 5648 MASP1 mannan binding lectin serin... NR_033519.2 52.9% (many diffs)
10 human 5648 MASP1 mannan binding lectin serin... NM_001031849.2 51.5% 50.2% (many diffs)
11 human 5648 MASP1 mannan binding lectin serin... XM_011512991.2 51% 49.6% (many diffs)
12 human 5648 MASP1 mannan binding lectin serin... XM_006713701.2 48% 46.7% (many diffs)
13 mouse 17174 Masp1 mannan-binding lectin serin... XM_006521828.3 86% 89.4% (many diffs)
14 mouse 17174 Masp1 mannan-binding lectin serin... XM_006521829.3 44.6% 45.8% (many diffs)
15 mouse 17174 Masp1 mannan-binding lectin serin... XM_006521830.1 42.7% 44.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2253
ORF length:
2184
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GAGGTGGCTG CTTCTCTATT ATGCTCTGTG CTTCTCCCTG TCAAAGGCTT
121CAGCCCACAC CGTGGAGCTA AACAATATGT TTGGCCAGAT CCAGTCGCCT GGTTATCCAG
181ACTCCTATCC CAGTGATTCA GAGGTGACTT GGAATATCAC TGTCCCAGAT GGGTTTCGGA
241TCAAGCTTTA CTTCATGCAC TTCAACTTGG AATCCTCCTA CCTTTGTGAA TATGACTATG
301TGAAGGTAGA AACTGAGGAC CAGGTGCTGG CAACCTTCTG TGGCAGGGAG ACCACAGACA
361CAGAGCAGAC TCCCGGCCAG GAGGTGGTCC TCTCCCCTGG CTCCTTCATG TCCATCACTT
421TCCGGTCAGA TTTCTCCAAT GAGGAGCGTT TCACAGGCTT TGATGCCCAC TACATGGCTG
481TGGATGTGGA CGAGTGCAAG GAGAGGGAGG ACGAGGAGCT GTCCTGTGAC CACTACTGCC
541ACAACTACAT TGGCGGCTAC TACTGCTCCT GCCGCTTCGG CTACATCCTC CACACAGACA
601ACAGGACCTG CCGAGTGGAG TGCAGTGACA ACCTCTTCAC TCAAAGGACT GGGGTGATCA
661CCAGCCCTGA CTTCCCAAAC CCTTACCCCA AGAGCTCTGA ATGCCTGTAT ACCATCGAGC
721TGGAGGAGGG TTTCATGGTC AACCTGCAGT TTGAGGACAT ATTTGACATT GAGGACCATC
781CTGAGGTGCC CTGCCCCTAT GACTACATCA AGATCAAAGT TGGTCCAAAA GTTTTGGGGC
841CTTTCTGTGG AGAGAAAGCC CCAGAACCCA TCAGCACCCA GAGCCACAGT GTCCTGATCC
901TGTTCCATAG TGACAACTCG GGAGAGAACC GGGGCTGGAG GCTCTCATAC AGGGCTGCAG
961GAAATGAGTG CCCAGAGCTA CAGCCTCCTG TCCATGGGAA AATCGAGCCC TCCCAAGCCA
1021AGTATTTCTT CAAAGACCAA GTGCTCGTCA GCTGTGACAC AGGCTACAAA GTGCTGAAGG
1081ATAATGTGGA GATGGACACA TTCCAGATTG AGTGTCTGAA GGATGGGACG TGGAGTAACA
1141AGATTCCCAC CTGTAAAATT GTAGACTGTA GAGCCCCAGG AGAGCTGGAA CACGGGCTGA
1201TCACCTTCTC TACAAGGAAC AACCTCACCA CATACAAGTC TGAGATCAAA TACTCCTGTC
1261AGGAGCCCTA TTACAAGATG CTCAACAATA ACACAGGTAT ATATACCTGT TCTGCCCAAG
1321GAGTCTGGAT GAATAAAGTA TTGGGGAGAA GCCTACCCAC CTGCCTTCCA GAGTGTGGTC
1381AGCCCTCCCG CTCCCTGCCA AGCCTGGTCA AGAGGATCAT TGGGGGCCGA AATGCTGAGC
1441CCGGCCTCTT CCCGTGGCAG GCCCTGATAG TGGTGGAGGA CACTTCGAGA GTGCCAAATG
1501ACAAGTGGTT TGGGAGTGGG GCCCTGCTCT CTGCGTCCTG GATCCTCACA GCAGCTCATG
1561TGCTGCGCTC CCAGCGTAGA GACACCACGG TGATACCAGT CTCCAAGGAG CATGTCACCG
1621TCTACCTGGG CTTGCATGAT GTGCGAGACA AATCGGGGGC AGTCAACAGC TCAGCTGCCC
1681GAGTGGTGCT CCACCCAGAC TTCAACATCC AAAACTACAA CCACGATATA GCTCTGGTGC
1741AGCTGCAGGA GCCTGTGCCC CTGGGACCCC ACGTTATGCC TGTCTGCCTG CCAAGGCTTG
1801AGCCTGAAGG CCCGGCCCCC CACATGCTGG GCCTGGTGGC CGGCTGGGGC ATCTCCAATC
1861CCAATGTGAC AGTGGATGAG ATCATCAGCA GTGGCACACG GACCTTGTCA GATGTCCTAC
1921AGTATGTCAA GTTACCCGTG GTGCCTCACG CTGAGTGCAA AACTAGCTAT GAGTCCCGCT
1981CGGGCAATTA CAGCGTCACG GAGAACATGT TCTGTGCTGG CTACTACGAG GGCGGCAAAG
2041ACACGTGCCT TGGAGATAGC GGTGGGGCCT TTGTCATCTT TGATGACTTG AGCCAGCGCT
2101GGGTGGTGCA AGGCCTGGTG TCCTGGGGGG GACCTGAAGA ATGCGGCAGC AAGCAGGTCT
2161ATGGAGTCTA CACAAAGGTC TCCAATTACG TGGACTGGGT GTGGGAGCAG ATGGGCTTAC
2221CACAAAGTGT TGTGGAGCCC CAGGTGGAAC GGTTGCCAAC TTTCTTGTAC AAAGTtggca
2281ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)

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