Construct: ORF ccsbBroadEn_07090

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000622.1_s300c1, BRDN0000386094
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM1 (7168)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7168 TPM1 tropomyosin 1 NM_000366.6 99.7% 100% 180C>T;453C>A
2 human 7168 TPM1 tropomyosin 1 XM_017022538.2 99.7% 100% 180C>T;453C>A
3 human 7168 TPM1 tropomyosin 1 XM_006720667.4 99.6% 99.6% 180C>T;453C>A;852A>G
4 human 7168 TPM1 tropomyosin 1 NM_001365779.1 96% 94.3% (many diffs)
5 human 7168 TPM1 tropomyosin 1 NM_001018005.2 95.9% 94% (many diffs)
6 human 7168 TPM1 tropomyosin 1 XM_017022539.2 94.6% 93.6% (many diffs)
7 human 7168 TPM1 tropomyosin 1 XM_005254639.4 94.2% 90.6% (many diffs)
8 human 7168 TPM1 tropomyosin 1 NM_001018006.2 93.1% 92.2% (many diffs)
9 human 7168 TPM1 tropomyosin 1 NM_001365776.1 91% 88% (many diffs)
10 human 7168 TPM1 tropomyosin 1 NM_001365777.1 90.6% 85% (many diffs)
11 human 7168 TPM1 tropomyosin 1 NM_001018004.2 89.5% 86.6% (many diffs)
12 human 7168 TPM1 tropomyosin 1 NM_001301244.2 86.6% 84.5% (many diffs)
13 human 7168 TPM1 tropomyosin 1 XM_017022536.2 86.6% 78.9% (many diffs)
14 human 7168 TPM1 tropomyosin 1 NM_001018020.2 84.1% 82.7% (many diffs)
15 human 7168 TPM1 tropomyosin 1 XM_017022534.2 83.4% 74% (many diffs)
16 human 7168 TPM1 tropomyosin 1 NM_001365781.2 81% 75.8% (many diffs)
17 human 7168 TPM1 tropomyosin 1 XM_005254650.3 80.9% 75.5% (many diffs)
18 human 7168 TPM1 tropomyosin 1 NM_001018007.2 80.7% 77.1% (many diffs)
19 human 7168 TPM1 tropomyosin 1 XM_017022537.2 78.4% 70.7% (many diffs)
20 human 7168 TPM1 tropomyosin 1 NM_001365778.1 78.3% 75.4% (many diffs)
21 human 7168 TPM1 tropomyosin 1 NM_001365782.1 77.5% 70.3% (many diffs)
22 human 7168 TPM1 tropomyosin 1 NM_001330346.2 77.4% 70% (many diffs)
23 human 7168 TPM1 tropomyosin 1 NM_001330351.2 76.1% 69.6% (many diffs)
24 human 7168 TPM1 tropomyosin 1 XM_017022535.2 75.4% 65.8% (many diffs)
25 human 7168 TPM1 tropomyosin 1 NM_001330344.2 74.9% 68.2% (many diffs)
26 human 7168 TPM1 tropomyosin 1 NM_001018008.2 72.7% 64.1% (many diffs)
27 human 7168 TPM1 tropomyosin 1 NM_001365780.1 72.6% 61.5% (many diffs)
28 human 7168 TPM1 tropomyosin 1 NM_001301289.2 71.6% 62.7% (many diffs)
29 human 7168 TPM1 tropomyosin 1 XM_005254646.2 69.9% 58.3% (many diffs)
30 human 7168 TPM1 tropomyosin 1 XM_017022540.1 66.9% 53.5% (many diffs)
31 human 7168 TPM1 tropomyosin 1 XM_024450040.1 46.8% 40.5% (many diffs)
32 human 7168 TPM1 tropomyosin 1 XR_002957675.1 26.6% (many diffs)
33 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164251.1 94.7% 99.2% (many diffs)
34 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164248.1 91% 93.6% (many diffs)
35 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242715.2 89.5% 90.2% (many diffs)
36 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164250.1 87.9% 91.9% (many diffs)
37 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511043.3 87.3% 89.4% (many diffs)
38 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511040.3 86% 84.7% (many diffs)
39 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164255.1 85.3% 87.6% (many diffs)
40 mouse 22003 Tpm1 tropomyosin 1, alpha NM_024427.4 84.4% 86.2% (many diffs)
41 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511041.1 83.9% 84.1% (many diffs)
42 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511042.3 83.8% 83.8% (many diffs)
43 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242714.2 82.4% 80.5% (many diffs)
44 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511046.2 81.6% 83.4% (many diffs)
45 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511044.1 80.8% 82% (many diffs)
46 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511050.2 78.5% 75.5% (many diffs)
47 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511045.2 78.3% 77.8% (many diffs)
48 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164249.1 77.5% 76.4% (many diffs)
49 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164254.1 75% 69.6% (many diffs)
50 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242716.2 73.6% 66.6% (many diffs)
51 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511051.3 72.9% 69.3% (many diffs)
52 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164253.1 72.1% 67.9% (many diffs)
53 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164256.1 69.6% 63.7% (many diffs)
54 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164252.1 68.9% 62.4% (many diffs)
55 mouse 22003 Tpm1 tropomyosin 1, alpha XM_017313283.1 65.9% 65.1% (many diffs)
56 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379412.3 53.1% (many diffs)
57 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379413.1 37.6% (many diffs)
58 mouse 22003 Tpm1 tropomyosin 1, alpha XR_001778851.1 24% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
918
ORF length:
852
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CGCCATCAAG AAGAAGATGC AGATGCTGAA GCTCGACAAG GAGAACGCCT
121TGGATCGAGC TGAGCAGGCG GAGGCCGACA AGAAGGCGGC GGAAGACAGG AGCAAGCAGC
181TGGAAGATGA GCTGGTGTCA CTGCAAAAGA AACTCAAGGG CACCGAAGAT GAACTGGACA
241AATATTCTGA GGCTCTCAAA GATGCCCAGG AGAAGCTGGA GCTGGCAGAG AAAAAGGCCA
301CCGATGCTGA AGCCGACGTA GCTTCTCTGA ACAGACGCAT CCAGCTGGTT GAGGAAGAGT
361TGGATCGTGC CCAGGAGCGT CTGGCAACAG CTTTGCAGAA GCTGGAGGAA GCTGAGAAGG
421CAGCAGATGA GAGTGAGAGA GGCATGAAAG TCATTGAGAG TCGAGCCCAA AAAGATGAAG
481AAAAAATGGA AATTCAGGAG ATCCAACTGA AAGAGGCAAA GCACATTGCT GAAGATGCCG
541ACCGCAAATA TGAAGAGGTG GCCCGTAAGC TGGTCATCAT TGAGAGCGAC CTGGAACGTG
601CAGAGGAGCG GGCTGAGCTC TCAGAAGGCC AAGTCCGACA GCTGGAAGAA CAATTAAGAA
661TAATGGATCA GACCTTGAAA GCATTAATGG CTGCAGAGGA TAAGTACTCG CAGAAGGAAG
721ACAGATATGA GGAAGAGATC AAGGTCCTTT CCGACAAGCT GAAGGAGGCT GAGACTCGGG
781CTGAGTTTGC GGAGAGGTCA GTAACTAAAT TGGAGAAAAG CATTGATGAC TTAGAAGACG
841AGCTGTACGC TCAGAAACTG AAGTACAAAG CCATCAGCGA GGAGCTGGAC CACGCTCTCA
901ACGATATGAC TTCCATGTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
961gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)