Transcript: Mouse NM_001164254.1

Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.13, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Tpm1 (22003)
Length:
1183
CDS:
163..909

Additional Resources:

NCBI RefSeq record:
NM_001164254.1
NBCI Gene record:
Tpm1 (22003)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001164254.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000071536 GAAGATGCTGACCGGAAGTAT pLKO.1 520 CDS 100% 5.625 7.875 N Tpm1 n/a
2 TRCN0000351719 GAAGATGCTGACCGGAAGTAT pLKO_005 520 CDS 100% 5.625 7.875 N Tpm1 n/a
3 TRCN0000071537 CGGTGACGAACAACTTGAAGT pLKO.1 650 CDS 100% 4.950 6.930 N Tpm1 n/a
4 TRCN0000351720 CGGTGACGAACAACTTGAAGT pLKO_005 650 CDS 100% 4.950 6.930 N Tpm1 n/a
5 TRCN0000071535 AGCTGACGTAGCTTCTCTGAA pLKO.1 300 CDS 100% 4.950 3.465 N Tpm1 n/a
6 TRCN0000351799 AGCTGACGTAGCTTCTCTGAA pLKO_005 300 CDS 100% 4.950 3.465 N Tpm1 n/a
7 TRCN0000071534 GCAGAGAGATCAGTAACCAAA pLKO.1 778 CDS 100% 4.950 3.465 N Tpm1 n/a
8 TRCN0000062151 GCCCGTAAGCTGGTCATCATT pLKO.1 550 CDS 100% 5.625 3.938 N TPM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001164254.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07091 pDONR223 100% 88.5% 91.5% None (many diffs) n/a
2 ccsbBroad304_07091 pLX_304 0% 88.5% 91.5% V5 (many diffs) n/a
3 TRCN0000481549 GATCAAGGGTCTCTCCCCTACTAG pLX_317 63.1% 88.5% 91.5% V5 (many diffs) n/a
4 ccsbBroadEn_07090 pDONR223 100% 75% 69.6% None (many diffs) n/a
5 ccsbBroad304_07090 pLX_304 0% 75% 69.6% V5 (many diffs) n/a
6 TRCN0000492218 CAAACTCGTCTCTACCATCGTAAC pLX_317 61.1% 75% 69.6% V5 (many diffs) n/a
7 ccsbBroadEn_11198 pDONR223 100% 51.6% 58% None (many diffs) n/a
8 ccsbBroad304_11198 pLX_304 0% 51.6% 58% V5 (many diffs) n/a
9 TRCN0000473724 CAACCCTTACTTATGAATACCCCC pLX_317 86% 51.6% 58% V5 (many diffs) n/a
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