Construct: ORF ccsbBroadEn_08451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012757.1_s300c1, BRDN0000396514
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM118A (55007)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55007 | FAM118A | family with sequence simila... | NM_001104595.2 | 99.7% | 99.4% | 385G>C;420G>A;716G>A |
| 2 | human | 55007 | FAM118A | family with sequence simila... | NM_001349913.1 | 99.7% | 99.4% | 385G>C;420G>A;716G>A |
| 3 | human | 55007 | FAM118A | family with sequence simila... | NM_017911.4 | 99.7% | 99.4% | 385G>C;420G>A;716G>A |
| 4 | human | 55007 | FAM118A | family with sequence simila... | NM_001349914.1 | 99.4% | 99.1% | (many diffs) |
| 5 | human | 55007 | FAM118A | family with sequence simila... | NM_001349916.1 | 95.9% | 95.6% | (many diffs) |
| 6 | human | 55007 | FAM118A | family with sequence simila... | NM_001349915.1 | 79.1% | 78.9% | 300_301ins222;494G>A |
| 7 | human | 55007 | FAM118A | family with sequence simila... | XM_017028843.1 | 76.1% | 76% | 1_42del;342_343ins222;536G>A |
| 8 | human | 55007 | FAM118A | family with sequence simila... | XM_017028842.1 | 75.9% | 75.8% | (many diffs) |
| 9 | human | 55007 | FAM118A | family with sequence simila... | XM_017028844.1 | 73% | 72.8% | (many diffs) |
| 10 | human | 55007 | FAM118A | family with sequence simila... | XM_017028850.1 | 59.6% | 59.5% | (many diffs) |
| 11 | human | 55007 | FAM118A | family with sequence simila... | XM_017028851.1 | 59.6% | 59.5% | (many diffs) |
| 12 | human | 55007 | FAM118A | family with sequence simila... | XM_017028852.1 | 59.6% | 59.5% | (many diffs) |
| 13 | human | 55007 | FAM118A | family with sequence simila... | XM_017028846.1 | 59.5% | 59.4% | (many diffs) |
| 14 | human | 55007 | FAM118A | family with sequence simila... | XM_017028847.2 | 59.5% | 59.4% | (many diffs) |
| 15 | human | 55007 | FAM118A | family with sequence simila... | XM_017028848.1 | 59.5% | 59.4% | (many diffs) |
| 16 | human | 55007 | FAM118A | family with sequence simila... | XM_017028849.1 | 59.5% | 59.4% | (many diffs) |
| 17 | human | 55007 | FAM118A | family with sequence simila... | XM_017028858.1 | 58.1% | 50.5% | (many diffs) |
| 18 | human | 55007 | FAM118A | family with sequence simila... | XM_017028857.1 | 58% | 50.4% | (many diffs) |
| 19 | human | 55007 | FAM118A | family with sequence simila... | XM_017028841.1 | 57.8% | 57.6% | (many diffs) |
| 20 | human | 55007 | FAM118A | family with sequence simila... | XM_011530247.3 | 57.6% | 57.5% | (many diffs) |
| 21 | human | 55007 | FAM118A | family with sequence simila... | XM_017028845.1 | 57.6% | 57.5% | (many diffs) |
| 22 | human | 55007 | FAM118A | family with sequence simila... | XM_024452254.1 | 56% | 48.6% | (many diffs) |
| 23 | human | 55007 | FAM118A | family with sequence simila... | XM_011530256.1 | 55.8% | 48.5% | (many diffs) |
| 24 | human | 55007 | FAM118A | family with sequence simila... | XM_024452255.1 | 54.6% | 47.9% | (many diffs) |
| 25 | human | 55007 | FAM118A | family with sequence simila... | XM_017028861.1 | 54.2% | 54% | 0_1ins489;227G>A |
| 26 | human | 55007 | FAM118A | family with sequence simila... | XR_001755254.1 | 42.5% | (many diffs) | |
| 27 | human | 55007 | FAM118A | family with sequence simila... | XM_011530254.3 | 40.9% | 40.2% | (many diffs) |
| 28 | human | 55007 | FAM118A | family with sequence simila... | XM_024452256.1 | 39.2% | 38.2% | (many diffs) |
| 29 | human | 55007 | FAM118A | family with sequence simila... | XM_024452257.1 | 39.2% | 38.2% | (many diffs) |
| 30 | human | 55007 | FAM118A | family with sequence simila... | XR_001755255.1 | 28.7% | (many diffs) | |
| 31 | human | 55007 | FAM118A | family with sequence simila... | XM_017028856.2 | 28.6% | 23.9% | (many diffs) |
| 32 | human | 55007 | FAM118A | family with sequence simila... | XR_002958707.1 | 28.6% | (many diffs) | |
| 33 | human | 55007 | FAM118A | family with sequence simila... | XR_001755256.1 | 27.6% | (many diffs) | |
| 34 | human | 55007 | FAM118A | family with sequence simila... | NR_146323.1 | 27.5% | (many diffs) | |
| 35 | human | 55007 | FAM118A | family with sequence simila... | XR_002958708.1 | 23.1% | (many diffs) | |
| 36 | human | 55007 | FAM118A | family with sequence simila... | XR_002958705.1 | 19.8% | (many diffs) | |
| 37 | human | 55007 | FAM118A | family with sequence simila... | XR_002958706.1 | 19.2% | (many diffs) | |
| 38 | human | 55007 | FAM118A | family with sequence simila... | XR_002958709.1 | 19.1% | (many diffs) | |
| 39 | mouse | 73225 | Fam118a | family with sequence simila... | NM_133750.4 | 86.7% | 91.8% | (many diffs) |
| 40 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521483.2 | 86.7% | 91.8% | (many diffs) |
| 41 | mouse | 73225 | Fam118a | family with sequence simila... | XM_017316757.1 | 86.5% | 91.6% | (many diffs) |
| 42 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521482.3 | 85% | 90.1% | (many diffs) |
| 43 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521481.3 | 84.8% | 89.8% | (many diffs) |
| 44 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521478.3 | 83.4% | 88% | (many diffs) |
| 45 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521479.3 | 83.4% | 88% | (many diffs) |
| 46 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521480.3 | 83.4% | 88% | (many diffs) |
| 47 | mouse | 73225 | Fam118a | family with sequence simila... | XM_011245743.2 | 82.1% | 86.5% | (many diffs) |
| 48 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521474.3 | 81.9% | 86.4% | (many diffs) |
| 49 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521475.3 | 81.9% | 86.4% | (many diffs) |
| 50 | mouse | 73225 | Fam118a | family with sequence simila... | XM_006521476.3 | 81.9% | 86.4% | (many diffs) |
| 51 | mouse | 73225 | Fam118a | family with sequence simila... | XR_001781571.1 | 33.2% | (many diffs) | |
| 52 | mouse | 73225 | Fam118a | family with sequence simila... | XR_384052.3 | 32% | (many diffs) | |
| 53 | mouse | 73225 | Fam118a | family with sequence simila... | XR_001781570.1 | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA TTCAGTGGAA AAGACAACAA ATAGAAGTGA ACAAAAATCC AGAAAGTTTT 121 TAAAAAGCCT CATCCGGAAA CAGCCCCAGG AACTGCTCCT GGTTATCGGG ACTGGCGTCA 181 GCGCAGCAGT GGCCCCCGGA ATCCCTGCCC TTTGCTCGTG GAGAAGCTGC ATCGAGGCCG 241 TCATCGAGGC TGCAGAGCAG CTGGAGGTGC TGCACCCCGG AGACGTCGCC GAGTTCCGGA 301 GGAAAGTGAC AAAGGACCGG GACCTGTTGG TTGTCGCCCA TGATCTGATC CGGAAGATGT 361 CACCTCGCAC AGGCGATGCC AAGCCCAGCT TCTTCCAGGA CTGCCTGATG GAGGTGTTTG 421 ACGACCTGGA GCAGCACATC CGGAGTCCTC TGGTGCTGCA GTCGATCCTC AGCCTGATGG 481 ACAGAGGCGC CATGGTCCTG ACCACCAACT ATGACAACCT GCTGGAGGCC TTTGGCCGGC 541 GGCAGAACAA GCCCATGGAG TCCCTGGACT TGAAGGACAA GACCAAGGTC CTTGAATGGG 601 CAAGAGGGCA CATGAAGTAC GGCGTCCTCC ACATTCACGG CCTCTACACG GACCCCTGCG 661 GGGTGGTGCT GGACCCATCG GGGTATAAAG ACGTCACTCA AGACGCAGAA GTCATGGAAG 721 TCCTCCAGAA CTTATACCGC ACCAAGTCCT TTCTGTTTGT GGGCTGTGGG GAGACCCTTC 781 ATGATCAGAT ATTCCAGGCC CTCTTTCTTT ACTCCGTGCC GAATAAGGTG GATTTGGAGC 841 ACTACATGCT TGTGCTGAAG GAGAATGAAG ACCATTTCTT TAAGCATCAG GCAGATATGC 901 TTCTGCACGG AATCAAAGTT GTATCCTACG GGGACTGTTT TGACCACTTT CCAGGATATG 961 TGCAAGACCT TGCCACTCAG ATCTGCAAAC AGCAAAGCCC AGATGCTGAT CGCGTGGACA 1021 GCACCACATT ATTGGGTAAT GCATGCCAGG ACTGTGCAAA GAGGAAGTTA GAAGAGAATG 1081 GAATTGAAGT TTCAAAAAAA CGCACACAAT CAGATACTGA TGATGCTGGA GGGTCTTGCC 1141 CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 1201 aac