Transcript: Human XM_017028850.1

PREDICTED: Homo sapiens family with sequence similarity 118 member A (FAM118A), transcript variant X15, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM118A (55007)
Length:
2901
CDS:
264..1283

Additional Resources:

NCBI RefSeq record:
XM_017028850.1
NBCI Gene record:
FAM118A (55007)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017028850.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194380 CCATGATCTGATCCGGAAGAT pLKO.1 536 CDS 100% 4.950 6.930 N Fam118a n/a
2 TRCN0000434843 GACCCTTCGTGATCAGATATT pLKO_005 749 CDS 100% 13.200 10.560 N FAM118A n/a
3 TRCN0000129352 CGAAACCGTCACATACACCAA pLKO.1 1453 3UTR 100% 2.640 2.112 N FAM118A n/a
4 TRCN0000415448 CTTTACTCCGTGCCGAATAAG pLKO_005 783 CDS 100% 13.200 9.240 N FAM118A n/a
5 TRCN0000129542 GCCCAAACGAAGAGGAATGTA pLKO.1 1404 3UTR 100% 5.625 3.938 N FAM118A n/a
6 TRCN0000130178 CGCACACAATCAGATACTGAT pLKO.1 1313 3UTR 100% 4.950 3.465 N FAM118A n/a
7 TRCN0000130418 GACACATTTCAGGTGGACTTT pLKO.1 1554 3UTR 100% 4.950 3.465 N FAM118A n/a
8 TRCN0000129205 CGAAGAGGAATGTATGGAGAA pLKO.1 1411 3UTR 100% 4.050 2.835 N FAM118A n/a
9 TRCN0000174961 GAAGACCATTTCTTTAAGCAT pLKO.1 843 CDS 100% 3.000 2.100 N Fam118a n/a
10 TRCN0000131030 CCAGAACTTATACCGCACCAA pLKO.1 701 CDS 100% 2.640 1.848 N FAM118A n/a
11 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 2003 3UTR 100% 10.800 5.400 Y MRPS16 n/a
12 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 2003 3UTR 100% 10.800 5.400 Y CD3EAP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017028850.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08451 pDONR223 100% 59.6% 59.5% None (many diffs) n/a
2 ccsbBroad304_08451 pLX_304 0% 59.6% 59.5% V5 (many diffs) n/a
3 TRCN0000480532 TAGCATGTCAGCTTCCGAGAGTTT pLX_317 39.1% 59.6% 59.5% V5 (many diffs) n/a
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