Construct: ORF ccsbBroadEn_08640

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF006358.1_s300c1, BRDN0000396845
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: EIF4ENIF1 (56478)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	56478	EIF4ENIF1	eukaryotic translation init...	NM_001164502.2	99.9%	100%	1704C>T
2	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_005261686.3	82.2%	82.2%	297_785del;1477_1512del;2229C>T
3	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_005261687.3	82.2%	82.2%	297_785del;1477_1512del;2229C>T
4	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_005261688.3	82.2%	82.2%	297_785del;1477_1512del;2229C>T
5	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_011530281.3	82.2%	82.2%	297_785del;1477_1512del;2229C>T
6	human	56478	EIF4ENIF1	eukaryotic translation init...	NM_001164501.2	82.1%	82.1%	(many diffs)
7	human	56478	EIF4ENIF1	eukaryotic translation init...	NM_019843.4	82.1%	82.1%	(many diffs)
8	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_011530280.2	82.1%	82.1%	(many diffs)
9	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_017028864.2	82.1%	82.1%	(many diffs)
10	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_006724281.4	78.9%	78.9%	(many diffs)
11	human	56478	EIF4ENIF1	eukaryotic translation init...	XM_017028863.2	78.8%	78.8%	(many diffs)
12	human	56478	EIF4ENIF1	eukaryotic translation init...	XR_001755264.2	38.2%		(many diffs)
13	mouse	74203	Eif4enif1	eukaryotic translation init...	NM_001166547.1	72.2%	72.2%	(many diffs)
14	mouse	74203	Eif4enif1	eukaryotic translation init...	NM_023743.2	72.2%	72.2%	(many diffs)
15	mouse	74203	Eif4enif1	eukaryotic translation init...	XM_006514841.2	72.2%	72.2%	(many diffs)
16	mouse	74203	Eif4enif1	eukaryotic translation init...	XM_006514842.3	72.2%	72.2%	(many diffs)
17	mouse	74203	Eif4enif1	eukaryotic translation init...	XM_006514843.3	72.2%	72.2%	(many diffs)
18	mouse	74203	Eif4enif1	eukaryotic translation init...	NM_001166548.1	71.8%	71.3%	(many diffs)
19	mouse	74203	Eif4enif1	eukaryotic translation init...	NM_001166549.1	71.8%	71.3%	(many diffs)
20	mouse	74203	Eif4enif1	eukaryotic translation init...	XM_006514844.2	71.8%	71.3%	(many diffs)
21	mouse	74203	Eif4enif1	eukaryotic translation init...	XM_017314806.1	71.8%	71.3%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2499

ORF length:

2433

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGGA TAGGAGAAGT ATGGGTGAAA CAGAAAGTGG AGATGCTTTC CTTGACCTGA
121	AGAAGCCTCC TGCCTCCAAA TGCCCCCATC GCTATACAAA AGAAGAACTC TTGGATATAA
181	AAGAACTCCC CCATTCCAAA CAGAGGCCTT CATGCCTTTC TGAAAAATAT GACAGTGATG
241	GTGTCTGGGA CCCTGAGAAG TGGCATGCCT CTCTCTACCC AGCTTCAGGG CGGAGCTCAC
301	CAGTGGAAAG TCTGAAGAAA GAGTTGGATA CAGACCGGCC TTCCCTGGTG CGCAGGATAG
361	TAGGTATAGT AGAGTGCAAT GGAGGAGTGG CCGAAGAGGA TGAAGTGGAG GTCATCCTTG
421	CACAGGAGCC TGCGGCTGAT CAGGAAGTGC CAAGGGATGC TGTCTTGCCT GAGCAGTCCC
481	CAGGAGACTT TGACTTTAAT GAGTTCTTTA ACCTTGATAA GGTGCCATGC TTGGCTTCGA
541	TGATAGAAGA TGTTTTGGGA GAAGGGTCAG TCTCTGCCAG TCGGTTCAGT AGGTGGTTCT
601	CTAACCCGAG CAGATCAGGA AGCCGATCCA GCAGTCTTGG GTCAACACCA CATGAAGAGC
661	TAGAGAGACT TGCAGGTCTG GAGCAAGCCA TCCTCTCTCC TGGACAGAAC TCGGGGAATT
721	ACTTTGCTCC TATACCATTG GAAGACCATG CTGAAAATAA AGTGGATATT TTAGAAATGC
781	TACAGAAAGC CAAAGTGGAT TTGAAACCTC TTCTTTCCAG CCTTTCTGCA AATAAAGAAA
841	AACTTAAAGA AAGCTCACAT TCAGGGGTTG TGCTTTCAGT GGAGGAGGTA GAAGCAGGTC
901	TGAAGGGCTT GAAGGTTGAC CAGCAAGTGA AGAATTCAAC TCCCTTCATG GCAGAACACC
961	TAGAAGAGAC CTTGAGTGCC GTAACCAACA ATCGACAACT GAAGAAAGAC GGAGACATGA
1021	CTGCGTTCAA CAAGCTAGTG AGCACAATGA AGCGAAACCT TGAAAGCCAT TTGATGTCCC
1081	CTGCTGAGAT TCCAGGCCAG CCTGTCCCTA AGAACATCCT GCAGGAACTT CTGGGTCAAC
1141	CAGTTCAGAG ACCTGCTTCT TCCAATCTTC TGAGTGGCCT TATGGGGAGC TTGGAGCCTA
1201	CAACATCTTT ACTGGGCCAA AGAGCACCCT CTCCTCCCTT GTCACAGGTG TTTCAAACTC
1261	GAGCAGCCTC AGCTGACTAC CTTCGCCCAA GAATACCATC ACCAATTGGT TTCACACCAG
1321	GACCACAGCA GCTACTCGGA GATCCATTCC AAGGCATGCG CAAACCCATG AGCCCCATCA
1381	CAGCCCAGCA GATGAGCCAG CTGGAGTTGC AACAGGCAGC TTTAGAAGGG CTGGCCTTGC
1441	CACATGACCT TGCTGTACAG GCAGCAAACT TCTACCAGCC TGGTTTTGGC AAACCACAGG
1501	TGGACAGAAC CAGAGATGGA TTCAGAAACA GGCAACAGCG AGTGACCAAG TCACCAGCAC
1561	CCGTGCATCG AGGGAATTCC TCTTCCCCTG CCCCTGCTGC CTCCATCACA AGCATGCTTT
1621	CTCCTTCCTT TACCCCTACC TCAGTGATTC GTAAGATGTA CGAGAGCAAA GAGAAAAGCA
1681	AGGAGGAGCC AGCATCTGGA AAAGCAGCTC TTGGTGACAG TAAAGAGGAT ACTCAGAAGG
1741	CCAGTGAAGA AAACCTCCTG TCATCCAGTT CTGTACCCAG TGCCGATCGA GACTCTTCTC
1801	CCACTACAAA TTCCAAACTG TCAGCATTAC AGAGGTCTTC GTGTTCCACC CCACTGTCCC
1861	AGGCCAACCG TTACACCAAA GAACAAGATT ATCGACCTAA AGCAACTGGG AGAAAAACAC
1921	CCACCTTGGC ATCCCCAGTT CCTACAACAC CTTTTCTCCG CCCTGTCCAC CAAGTTCCCC
1981	TTGTCCCCCA TGTCCCTATG GTTAGGCCTG CTCACCAGCT TCACCCAGGG TTGGTACAGA
2041	GGATGCTGGC CCAGGGAGTA CATCCACAGC ATCTTCCAAG TTTGCTCCAA ACTGGTGTGC
2101	TTCCTCCTGG GATGGACTTG AGTCATTTAC AGGGAATATC TGGCCCCATC CTGGGTCAGC
2161	CCTTTTACCC TTTACCTGCT GCTAGTCACC CTCTCTTAAA CCCTCGTCCT GGAACACCTC
2221	TGCATCTGGC AATGGTGCAA CAGCAGCTAC AGCGCTCAGT TCTGCATCCT CCAGGCTCTG
2281	GTTCCCATGC AGCAGCTGTC AGCGTTCAGA CAACCCCTCA GAACGTGCCC AGCCGGTCAG
2341	GCCTGCCCCA CATGCACTCC CAGCTGGAGC ATCGCCCCAG CCAGAGGAGC AGCTCCCCTG
2401	TGGGCCTTGC CAAATGGTTT GGCTCAGATG TGCTACAGCA ACCCCTGCCC TCCATGCCCG
2461	CCAAAGTTAT CAGTGTAGAT GAATTGGAAT ACCGACAGTG CCCAACTTTC GTGTACAAAG
2521	Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

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