Construct: ORF ccsbBroadEn_08785

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003452.1_s300c1, BRDN0000392640
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNB4 (59345)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 59345 GNB4 G protein subunit beta 4 NM_021629.4 99.8% 99.7% 11T>C;117T>C
2 human 59345 GNB4 G protein subunit beta 4 XM_005247692.2 99.8% 99.7% 11T>C;117T>C
3 human 59345 GNB4 G protein subunit beta 4 XM_006713721.2 99.8% 99.7% 11T>C;117T>C
4 human 2782 GNB1 G protein subunit beta 1 NM_002074.5 75.8% 90.5% (many diffs)
5 human 2782 GNB1 G protein subunit beta 1 XM_017001059.2 75.8% 90.5% (many diffs)
6 human 2782 GNB1 G protein subunit beta 1 XM_017001060.2 75.8% 90.5% (many diffs)
7 human 2782 GNB1 G protein subunit beta 1 XM_017001061.2 75.8% 90.5% (many diffs)
8 human 2782 GNB1 G protein subunit beta 1 XM_024446495.1 75.8% 90.5% (many diffs)
9 mouse 14696 Gnb4 guanine nucleotide binding ... NM_001348062.1 85.1% 96.4% (many diffs)
10 mouse 14696 Gnb4 guanine nucleotide binding ... NM_001348104.1 85.1% 96.4% (many diffs)
11 mouse 14696 Gnb4 guanine nucleotide binding ... NM_013531.5 85.1% 96.4% (many diffs)
12 mouse 14696 Gnb4 guanine nucleotide binding ... XM_006535403.3 85.1% 96.4% (many diffs)
13 mouse 14688 Gnb1 guanine nucleotide binding ... XM_017319977.2 77.1% 90.5% (many diffs)
14 mouse 14696 Gnb4 guanine nucleotide binding ... NM_001348105.1 76.3% 86.1% (many diffs)
15 mouse 14696 Gnb4 guanine nucleotide binding ... XM_006535404.3 62.2% 70.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1086
ORF length:
1020
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CGAACCGGAA CAGTTGAGGC AAGAAGCAGA ACAACTGCGG AATCAGATTC
121AGGATGCTCG GAAAGCATGT AATGATGCAA CGCTTGTTCA GATTACATCA AATATGGACT
181CCGTGGGTCG AATACAAATG CGAACAAGAC GTACACTGAG GGGCCACCTA GCTAAAATCT
241ATGCTATGCA TTGGGGATAC GATTCCAGGC TGCTAGTCAG TGCTTCTCAA GATGGAAAAT
301TAATTATTTG GGATAGCTAT ACAACAAATA AGATGCATGC TATTCCTTTG AGGTCCTCCT
361GGGTGATGAC CTGTGCTTAT GCTCCCTCTG GTAATTATGT TGCCTGTGGA GGCTTGGACA
421ACATCTGCTC TATATATAAC TTAAAGACCA GAGAGGGAAA TGTGAGAGTA AGCCGAGAGT
481TGCCAGGTCA CACAGGGTAC TTGTCCTGCT GTCGTTTTTT AGATGACAGC CAAATTGTTA
541CAAGTTCAGG AGATACAACT TGTGCTTTAT GGGACATCGA AACTGCCCAG CAGACCACCA
601CATTCACTGG GCATTCTGGA GATGTGATGA GTCTTTCTTT GAGTCCTGAC ATGAGGACTT
661TTGTTTCTGG TGCTTGTGAT GCCTCTTCCA AATTATGGGA TATTCGAGAT GGAATGTGTA
721GACAGTCTTT CACGGGACAT GTCTCAGATA TCAATGCTGT CAGTTTTTTC CCAAATGGAT
781ATGCCTTCGC CACTGGCTCT GATGATGCCA CTTGCCGGCT CTTTGACCTT CGTGCAGATC
841AAGAGTTATT ATTGTATTCT CATGACAATA TCATCTGTGG AATCACTTCT GTAGCCTTCT
901CAAAAAGTGG GCGTCTCTTG TTGGCTGGTT ACGATGACTT TAATTGTAAT GTATGGGACA
961CGCTAAAAGG AGATCGTGCA GGTGTCCTTG CTGGTCATGA CAACCGTGTG AGCTGCTTAG
1021GTGTAACTGA TGATGGCATG GCTGTGGCAA CAGGCTCTTG GGACAGTTTT CTTAGAATCT
1081GGAATTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1141gttgcaacga ac

Download FASTA (ORF) (Full)