Construct: ORF ccsbBroadEn_09245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014581.1_s300c1, BRDN0000390468
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERAC1 (84947)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84947 | SERAC1 | serine active site containi... | NM_032861.4 | 99.8% | 99.8% | 249C>T;1627T>A |
| 2 | human | 84947 | SERAC1 | serine active site containi... | XM_024446573.1 | 99.8% | 99.8% | 249C>T;1627T>A |
| 3 | human | 84947 | SERAC1 | serine active site containi... | XM_011536196.3 | 97.2% | 90.6% | (many diffs) |
| 4 | human | 84947 | SERAC1 | serine active site containi... | XM_006715586.3 | 89.1% | 89.1% | 0_1ins210;39C>T;1417T>A |
| 5 | human | 84947 | SERAC1 | serine active site containi... | XM_011536198.3 | 89.1% | 89.1% | 0_1ins210;39C>T;1417T>A |
| 6 | human | 84947 | SERAC1 | serine active site containi... | NR_073096.2 | 74.2% | (many diffs) | |
| 7 | human | 84947 | SERAC1 | serine active site containi... | XR_942606.2 | 48.3% | (many diffs) | |
| 8 | human | 84947 | SERAC1 | serine active site containi... | XR_001743697.2 | 47.6% | (many diffs) | |
| 9 | mouse | 321007 | Serac1 | serine active site containi... | NM_001111017.1 | 84.2% | 86.6% | (many diffs) |
| 10 | mouse | 321007 | Serac1 | serine active site containi... | NM_177311.4 | 80.5% | 83.1% | (many diffs) |
| 11 | mouse | 321007 | Serac1 | serine active site containi... | XM_006523234.3 | 79.2% | 81.1% | (many diffs) |
| 12 | mouse | 321007 | Serac1 | serine active site containi... | XM_006523235.3 | 75.2% | 76.7% | (many diffs) |
| 13 | mouse | 321007 | Serac1 | serine active site containi... | XM_017317508.1 | 71.6% | 73.2% | (many diffs) |
| 14 | mouse | 321007 | Serac1 | serine active site containi... | XM_006523236.3 | 65.3% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2028
- ORF length:
- 1962
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC CCTGGCTGCT TATTGCGTCA TCTGTTGCAG AAGAATAGGA ACCTCTACTT 121 CCCCACCAAA AAGTGGCACA CACTGGAGAG ATATCAGAAA TATAATAAAG TTTACTGGAT 181 CACTTATTTT AGGAGGTTCT TTATTTCTTA CATATGAAGT TCTGGCCCTG AAGAAGGCTG 241 TGACATTAGA TACTCAAGTG GTAGAACGAG AAAAAATGAA GTCATATATA TATGTGCACA 301 CAGTTTCTTT AGATAAAGGA GAAAATCATG GTATTGCCTG GCAGGCAAGA AAAGAACTTC 361 ACAAAGCAGT AAGAAAAGTA TTGGCAACAT CAGCCAAGAT ACTGCGGAAT CCATTTGCTG 421 ATCCTTTTAG TACAGTTGAT ATAGAAGATC ATGAGTGTGC TGTGTGGCTG CTCCTACGGA 481 AGAGCAAGTC AGATGACAAA ACCACGCGAC TCGAGGCTGT GCGGGAAATG TCGGAGACCC 541 ATCACTGGCA TGATTACCAG TATAGGATAA TTGCTCAAGC CTGTGATCCG AAAACTCTTA 601 TTGGTTTGGC ACGAAGCGAA GAGAGTGATC TTCGCTTTTT TCTCCTACCA CCTCCTTTGC 661 CATCTTTAAA AGAAGATTCT TCCACTGAAG AAGAGCTCAG ACAGTTGCTG GCTTCCTTAC 721 CTCAAACAGA GCTAGATGAG TGTATCCAGT ATTTTACATC TTTGGCTCTT AGTGAAAGCA 781 GTCAGAGTCT AGCTGCTCAG AAGGGTGGTT TATGGTGTTT TGGAGGAAAT GGACTTCCTT 841 ATGCTGAAAG TTTTGGAGAA GTTCCTTCAG CAACAGTGGA AATGTTCTGT TTAGAAGCTA 901 TAGTAAAACA TTCTGAGATA TCCACACATT GTGATAAAAT CGAAGCAAAT GGAGGCCTGC 961 AGCTACTTCA GAGGCTGTAC CGACTTCACA AGGACTGCCC TAAAGTACAG AGAAATATAA 1021 TGCGTGTCAT TGGAAATATG GCTTTGAATG AACATCTTCA TTCTTCTATA GTTCGCTCAG 1081 GCTGGGTTTC CATCATGGCA GAAGCAATGA AATCTCCCCA CATTATGGAG TCCTCACACG 1141 CTGCCAGAAT CCTGGCAAAT CTAGACCGAG AAACTGTGCA AGAAAAATAT CAGGATGGCG 1201 TATATGTGCT GCATCCCCAA TATCGAACAA GTCAGCCCAT TAAAGCAGAT GTCCTTTTTA 1261 TTCATGGCCT TATGGGAGCA GCATTCAAAA CATGGCGCCA GCAGGACAGT GAGCAGGCTG 1321 TAATTGAAAA ACCTATGGAG GATGAAGACA GATATACGAC GTGCTGGCCC AAGACATGGT 1381 TAGCAAAAGA CTGTCCTGCT CTCCGAATTA TATCTGTGGA GTATGACACC AGCCTCAGCG 1441 ACTGGAGAGC AAGGTGCCCT ATGGAAAGAA AGTCCATTGC ATTCAGAAGC AACGAACTTC 1501 TTAGGAAGCT CAGAGCTGCT GGTGTTGGGG ATAGGCCAGT GGTTTGGATA TCACATAGCA 1561 TGGGAGGTCT TCTTGTCAAA AAGATGCTGT TGGAAGCCTC TACGAAGCCA GAAATGAGTA 1621 CTGTTATCAA CAATACCAGA GGAATAATTT TTTATAGTGT CCCTCATCAT GGATCACGTT 1681 TGGCTGAATA CACTGTTAAT ATTCGCTATC TTCTCTTCCC CTCGTTGGAA GTCAAAGAAC 1741 TCAGCAAGGA TTCTCCTGCA CTTAAAACAC TACAAGATGA CTTTCTGGAG TTTGCTAAAG 1801 ACAAAAACTT CCAGGTGCTG AATTTTGTGG AAACACTACC AACCTACATT GGCAGCATGA 1861 TTAAGCTCCA TGTGGTACCT GTGGAATCAG CAGATTTAGG CATTGGAGAT CTAATTCCTG 1921 TGGATGTTAA CCATTTGAAC ATTTGTAAGC CAAAGAAAAA GGATGCTTTT TTGTACCAGC 1981 GTACTTTACA ATTCATTCGT GAAGCTTTAG CCAAAGACCT TGAAAACTAC CCAACTTTCT 2041 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac